Triticum aestivum; field sprouting; falling number; amylases; peptidases; endoxylanases
Abstract :
[en] To date, research on preharvest sprouted (PHS) wheat has mostly been conducted on kernels germinated under laboratory conditions, which differ widely from conditions in the field. To obtain detailed knowledge of the evolution of hydrolytic enzyme activities in PHS wheat Triticum aestivum), a broad collection of samples from three varieties was obtained by harvesting before, at, and after maturity. Delaying harvest time coupled with periods of heavy rainfall caused sprouting in the kernels, observed as a drop in Falling Number and an increase in α-amylase activity. The appearance of α- and β-amylase, peptidase, and endoxylanase activity during field sprouting was independent from each other. Consequently, Falling Number could not be used to predict activity of other hydrolytic enzymes. When differentiating endogenous from kernel-associated microbial enzymes, results showed that α- and β-amylase and peptidase activity of PHS kernels were predominantly of endogenous origin, whereas endoxylanase activity was largely from microbial origin.
Disciplines :
Food science Biotechnology Agriculture & agronomy
Author, co-author :
Olaerts, Heleen; Katholieke Universiteit Leuven - KUL > Laboratory of food chemistry and biochemistry
Roye, Chiara; Katholieke Universiteit Leuven - KUL > Laboratory of food chemistry and biochemistry
Derde, Liesbeth; Katholieke Universiteit Leuven - KUL > Laboratory of food chemistry and biochemistry
Sinnaeve, Georges; Centre Wallon de recherche agronomique - CRA-W > Valorisation des productions
Meza Morales, Walter ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Phytotechnie des régions tempérées
Bodson, Bernard ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Phytotechnie des régions tempérées
Courtin, Christophe; Katholieke Universiteit Leuven - KUL > Laboratory of food chemistry and biochemistry
Language :
English
Title :
Evolution and Distribution of Hydrolytic Enzyme Activities during Preharvest Sprouting of Wheat (Triticum aestivum) in the Field
Publication date :
24 June 2016
Journal title :
Journal of Agricultural and Food Chemistry
ISSN :
0021-8561
eISSN :
1520-5118
Publisher :
American Chemical Society, Washington, United States - District of Columbia
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