Article (Scientific journals)
Reduced sputum expression of interferon-stimulated genes in severe COPD.
Hilzendeger, Clarissa; da Silva, Jane; HENKET, Monique et al.
2016In International Journal of Chronic Obstructive Pulmonary Disease, 11, p. 1485-94
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Keywords :
COPD; innate immunity; interferon-stimulated genes; interferon-beta; interferon-lambda1
Abstract :
[en] BACKGROUND: Exacerbations of COPD are frequent and commonly triggered by respiratory tract infections. The purpose of our study was to investigate innate immunity in stable COPD patients. METHODS: Induced sputum was collected from 51 stable consecutive COPD patients recruited from the COPD Clinic of CHU Liege and 35 healthy subjects. Expression of interferons beta (IFN-beta) and lambda1 (IL-29), IFN-stimulated genes (ISGs) MxA, OAS, and viperin were measured in total sputum cells by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The presence of Picornaviruses was assessed by RT-PCR, while potential pathogenic microorganisms (PPM) were identified by sputum bacteriology. RESULTS: Expression of IL-29 was found in 16 of 51 COPD patients (31%) and in nine of 35 healthy subjects (26%), while IFN-beta was detected in six of 51 COPD patients (12%) and in two of 35 healthy subjects (6%). ISGs were easily detectable in both groups. In the whole group of COPD patients, OAS expression was decreased (P<0.05), while that of viperin was increased (P<0.01) compared to healthy subjects. No difference was found with respect to MxA. COPD patients from group D of Global Initiative for Chronic Obstructive Lung Disease (GOLD) had reduced expression of all three ISGs (P<0.01 for MxA, P<0.05 for OAS, and P<0.01 for viperin) as compared to those of group B patients. Picornaviruses were detected in eight of 51 (16%) COPD patients vs four of 33 (12%) healthy subjects, while PPM were detected in seven of 39 (18%) COPD patients and associated with raised sputum neutrophil counts. IFN-beta expression was raised when either picornavirus or PPM were detected (P=0.06), but no difference was seen regarding IL-29 or ISGs. CONCLUSION: ISGs expression was reduced in severe COPD that may favor exacerbation and contribute to disease progress by altering response to infection.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Hilzendeger, Clarissa ;  Université de Liège > Département des sciences cliniques > Pneumologie - Allergologie
da Silva, Jane
HENKET, Monique ;  Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
SCHLEICH, FLorence ;  Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
CORHAY, Jean-Louis ;  Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
Kebadze, Tatiana
Edwards, Michael R.
Mallia, Patrick
Johnston, Sebastian Lennox
Louis, Renaud ;  Université de Liège > Département des sciences cliniques > Pneumologie - Allergologie
Language :
English
Title :
Reduced sputum expression of interferon-stimulated genes in severe COPD.
Publication date :
30 June 2016
Journal title :
International Journal of Chronic Obstructive Pulmonary Disease
ISSN :
1176-9106
eISSN :
1178-2005
Publisher :
Dove Medical Press Ltd, New Zealand
Volume :
11
Pages :
1485-94
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 September 2016

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