[en] Calcium carbonate particles are promising candidates as proteins carriers for their controlled delivery in the body. The present paper aims at investigating the protein encapsulation by in situ precipitation of calcium carbonate particles prepared by a process based on supercritical CO2 and using a new type of degradable well-defined double hydrophilic block copolymers composed of poly(ethylene oxide) and polyphosphoester blocks acting as templating agent for the calcium carbonate. For this study, lysozyme was chosen as a model for therapeutic protein for its availability and ease of detection. It was found that by this green process, loading into the CaCO3 microparticles with a diameter about 2 mm can be obtained as determined by scanning electron microscopy. A protein loading up to 6.5% active lysozyme was measured by a specific bioassay (Micrococcus lysodeikticus). By encapsulating fluorescent-labelled lysozyme (lysozyme-FITC), the confocal microscopy images confirmed its encapsulation and suggested a core–shell distribution of lysozyme into CaCO3, leading to a release profile reaching a steady state at 59% of release after 90 min.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Ergül, Zeynep ; University of Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Cordonnier, Thomas; University of Angers France, INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS
Debuigne, Antoine ; University of Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Clavignac, Brice; University of Angers France, INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS
Jérôme, Christine ; University of Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Boury, Frank; University of Angers France, INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS
Language :
English
Title :
Protein encapsulation and release from PEO-b-polyphosphoester templated calcium carbonate particles
Publication date :
20 November 2016
Journal title :
International Journal of Pharmaceutics
ISSN :
0378-5173
eISSN :
1873-3476
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
513
Pages :
130-1
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique The European Community in the frame of the Erasmus Mundus Joint Doctorate Program Nanofar
Barry, J.J.A., Silva, M.M.C.G., Popov, V.K., Shakesheff, K.M., Howdle, S.M., Supercritical carbon dioxide: putting the fizz into biomaterials (2006) Philos. Trans. A: Math. Phys. Eng. Sci., 364, pp. 249-261
Beuvier, T., Calvignac, B., Delcroix, G.J.-R., Tran, M.K., Kodjikian, S., Delorme, N., Bardeau, J.-F., Boury, F., Synthesis of hollow vaterite CaCO3 microspheres in supercritical carbon dioxide medium (2011) J. Mater. Chem., 21, pp. 9757-9761
Bezemer, J.M., Radersma, R., Grijpma, D.W., Dijkstra, P.J., Blitterswijk, C.A., Van Feijen, J., Microspheres for protein delivery prepared from amphiphilic multiblock copolymers 1: influence of preparation techniques on particle characteristics and protein delivery (2000) J. Control Release, 67, pp. 233-248
Bhamidipati, M., Scurto, A.M., Detamore, M.S., The future of carbon dioxide for polymer processing in tissue engineering (2013) Tissue Eng. Part B Rev., 19, pp. 221-232
Bouchard, A., Jovanović, N., Jiskoot, W., Mendes, E., Witkamp, G.-J., Crommelin a, D.J., Hofland, G.W., Lysozyme particle formation during supercritical fluid drying: particle morphology and molecular integrity (2007) J. Supercrit. Fluids, 40, pp. 293-307
Cölfen, H., Antonietti, M., Crystal design of calcium carbonate microparticles using double-hydrophilic block copolymers (1998) Langmuir, 14, pp. 582-589
Cölfen, H., Qi, L., A systematic examination of the morphogenesis of calcium carbonate in the presence of a double-hydrophilic block copolymer (2001) Chem. A: Eur. J., 7, pp. 106-116
Chavez Panduro, E.A., Beuvier, T., Fernández Martínez, M., Hassani, L., Calvignac, B., Boury, F., Gibaud, A., Small-angle X-ray scattering analysis of porous powders of CaCO3 (2012) J. Appl. Crystallogr., 45, pp. 881-889
Cheang, T., Wang, S., Hu, Z., Xing, Z.-H., Chang, G., Yao, C., Liu, Y., Xu, A.-W., Calcium carbonate/CaIP6 nanocomposite particles as gene delivery vehicles for human vascular smooth muscle cells (2010) J. Mater. Chem., 20, pp. 8050-8055
Cocero, M.J., Martín, Á., Mattea, F., Varona, S., Encapsulation and co-precipitation processes with supercritical fluids: fundamentals and applications (2009) J. Supercrit. Fluids, 47, pp. 546-555
Davies, O.R., Lewis, A.L., Whitaker, M.J., Tai, H., Shakesheff, K.M., Howdle, S.M., Applications of supercritical CO2 in the fabrication of polymer systems for drug delivery and tissue engineering (2008) Adv. Drug Deliv. Rev., 60, pp. 373-387
Deliberador, T.M., Giovanini, A.F., Ricardo, T., Zielak, J.C., Moro, A., Filho, F.B., Rychuv, F., Storrer, C.L.M., Immunoexpression of PPAR- g and osteocalcin proteins for bone repair of critical-Size defects treated with fragmented autogenous abdominal adipose tissue graft (2014) Braz. Dent. J., 25, pp. 179-185
Fujiwara, M., Shiokawa, K., Morigaki, K., Zhu, Y., Nakahara, Y., Calcium carbonate microcapsules encapsulating biomacromolecules (2008) Chem. Eng. J., 137, pp. 14-22
Fujiwara, M., Shiokawa, K., Araki, M., Ashitaka, N., Morigaki, K., Kubota, T., Nakahara, Y., Encapsulation of proteins into CaCO3 by phase transition from vaterite to calcite (2010) Cryst. Growth Des., 10, pp. 4030-4037
Fujiwara, M., Shiokawa, K., Kubota, T., Morigaki, K., Preparation of calcium carbonate microparticles containing organic fluorescent molecules from vaterite (2014) Adv. Powder Technol., 25, pp. 1147-1154
Gao, Y.-X., Yu, S.-H., Guo, X.-H., Double hydrophilic block copolymer controlled growth and self-assembly of CaCO3 multilayered structures at the air/water interface (2006) Langmuir, 22, pp. 6125-6129
Hassani, L.N., Hindré, F., Beuvier, T., Calvignac, B., Lautram, N., Gibaud, A., Boury, F., Lysozyme encapsulation into nanostructured CaCO3 microparticles using a supercritical CO2 process and comparison with the normal route (2013) J. Mater. Chem. B, 1, pp. 4011-4019
He, X., Liu, T., Chen, Y., Cheng, D., Li, X., Xiao, Y., Feng, Y., Calcium carbonate nanoparticle delivering vascular endothelial growth factor-C siRNA effectively inhibits lymphangiogenesis and growth of gastric cancer in vivo (2008) Cancer Gene Ther., 15, pp. 193-202
Ibraheem, D., Agusti, G., Elaissari, A., Fessi, H., Preparation and characterization of albumin-loaded polycaprolactone nanoparticles for in vivo applications (2014) J. Colloid Sci. Biotechnol., 3, pp. 160-166
Ibraheem, D., Elaissari, A., Fessi, H., Administration strategies for proteins and peptides (2014) Int. J. Pharm., 477 (1-2), pp. 578-589
Kašparová, P., Antonietti, M., Cölfen, H., Double hydrophilic block copolymers with switchable secondary structure as additives for crystallization control (2004) Colloids Surfaces A: Physicochem. Eng. Asp., 250, pp. 153-162
Limin, Q., Jie, L., Jiming, M., Biomimetic morphogenesis of calcium carbonate in mixed solutions of surfactants and double-hydrophilic block copolymers (2002) Adv. Mater., 14, pp. 300-303
Meldrum, F.C., Cölfen, H., Controlling mineral morphologies and structures in biological and synthetic systems (2008) Chem. Rev., 108, pp. 4332-4432
Oláh, L., Tuba, F., Investigation of calcium carbonates enhanced poly(ε-caprolactone) materials for biomedical applications (2010) Macromol. Symp., 296, pp. 371-377
Penczek, S., Pretula, J., Kaluzynski, K., Poly (alkylene phosphates): from synthetic models of biomacromolecules and biomembranes toward polymer – inorganic hybrids (Mimicking biomineralization) (2005) Biomacromolecules, 6, pp. 547-551
Peng, C., Zhao, Q., Gao, C., Sustained delivery of doxorubicin by porous CaCO3 and chitosan/alginate multilayers-coated CaCO3 microparticles (2010) Colloids Surfaces A: Physicochem. Eng. Asp., 353, pp. 132-139
Petrov, A.I., Volodkin, D.V., Sukhorukov, G.B., Protein-calcium carbonate coprecipitation: a tool for protein encapsulation (2005) Biotechnol. Prog., 21, pp. 918-925
Ramsay, S.D., Pilliar, R.M., Santerre, J.P., Fabrication of a biodegradable calcium polyphosphate/polyvinyl-urethane carbonate composite for high load bearing osteosynthesis applications (2010) J. Biomed. Mater. Res. B: Appl. Biomater., 94, pp. 178-186
Rudloff, J., Antonietti, M., Cölfen, H., Pretula, J., Kaluzynski, K., Penczek, S., Double-hydrophilic block copolymers with monophosphate ester moieties as crystal growth modifiers of CaCO3 (2002) Macromol. Chem. Phys., 203, pp. 627-635
Schmidt, S., Volodkin, D., Microparticulate biomolecules by mild CaCO3 templating (2013) J. Mater. Chem. B, 1, pp. 1210-1218
Sedlak, M., Antonietti, M., Colfen, H., Synthesis of a new class of double-hydrophilic block copolymers with calcium binding capacity as builders and for biomimetic structure control of minerals (1998) Macromol. Chem. Phys., 199, pp. 247-254
Svenskaya, Y., Parakhonskiy, B., Haase, A., Atkin, V., Lukyanets, E., Gorin, D., Antolini, R., Anticancer drug delivery system based on calcium carbonate particles loaded with a photosensitizer (2013) Biophys. Chem., 182, pp. 11-15
Tevenson, C.Y.L.S., Restrelski, S.T.J.P., Alto, P., Precipitation of proteins in supercritical carbon dioxide (1996) J. Pharm. Sci., 85, pp. 586-594
Tran, M.-K., Swed, A., Boury, F., Preparation of polymeric particles in CO(2) medium using non-toxic solvents: formulation and comparisons with a phase separation method (2012) Eur. J. Pharm. Biopharm., 82, pp. 498-507
Tran, M.-K., Hassani, L.N., Calvignac, B., Beuvier, T., Hindré, F., Boury, F., Lysozyme encapsulation within PLGA and CaCO3 microparticles using supercritical CO2 medium (2013) J. Supercrit. Fluids, 79, pp. 159-169
Trushina, D.B., Bukreeva, T.V., Kovalchuk, M.V., Antipina, M.N., CaCO3 vaterite microparticles for biomedical and personal care applications (2014) Mater. Sci. Eng. C, 45, pp. 644-658
Ueno, Y., Futagawa, H., Takagi, Y., Ueno, A., Mizushima, Y., Drug-incorporating calcium carbonate nanoparticles for a new delivery system (2005) J. Control. Release, 103, pp. 93-98
Verhamme, I.A.M., Van Dedem, G.W.K., LAUWERS, A., Ionic-strength-dependent substrate inhibition of the lysis of Micrococcus luteus by hen egg-white lysozyme (1988) Eur. J. Biochem., 172, pp. 615-620
Volodkin, D.V., Larionova, N.I., Sukhorukov, G.B., Protein encapsulation via porous CaCO3 microparticles templating (2004) Biomacromolecules, 5, pp. 1962-1972
Volodkin, D.V., Petrov, A.I., Prevot, M., Sukhorukov, G.B., Matrix polyelectrolyte microcapsules: new system for macromolecule encapsulation (2004) Langmuir, 20, pp. 3398-3406
Volodkin, D., CaCO3 templated micro-beads and -capsules for bioapplications (2014) Adv. Colloid Interface Sci., 207, pp. 306-324
Wang, T.Y., Bruggeman, K.A., Sheean, R.K., Turner, B.J., Nisbet, D.R., Parish, C.L., Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair (2014) J. Biol. Chem., 289, pp. 15044-15051
Wei, W., Hu, G., Yu, D., Mcleish, T., Su, Z., Shen, Z., Preparation of hierarchical hollow CaCO3 particles and the application as anticancer drug carrier (2008) J. Am. Chem. Soc., 130, pp. 15808-15810
Whitaker, M.J., Hao, J., Davies, O.R., Serhatkulu, G., Stolnik-Trenkic, S., Howdle, S.M., Shakesheff, K.M., The production of protein-loaded microparticles by supercritical fluid enhanced mixing and spraying (2005) J. Control. Release, 101, pp. 85-92
Zeynep, E.Y., Antoine, D., Brice, C., Frank, B., Christine, J., Double hydrophilic polyphosphoester containing copolymers as efficient templating agents for calcium carbonate microparticles (2015) J. Mater. Chem. B, 3, pp. 7227-7236
Zhang, Y., Ma, P., Wang, Y., Du, J., Zhou, Q., Zhu, Z., Yang, X., Yuan, J., Biocompatibility of porous spherical calcium carbonate microparticles on hela cells (2012) World J. Nano Sci. Eng., 2, pp. 25-31
Zhang, Y., Biocompatibility of porous spherical calcium carbonate microparticles on hela cells (2012) World J. Nano Sci. Eng., 2, pp. 25-31
Zhao, D., Wang, C.-Q., Zhuo, R.-X., Cheng, S.-X., Modification of nanostructured calcium carbonate for efficient gene delivery (2014) Colloids Surf. B: Biointerfaces, 118, pp. 111-116
Zhu, S., Segura, T., Hydrogel-Based nanocomposites of therapeutic proteins for tissue repair (2014) Curr. Opin. Chem. Eng., 4, pp. 128-136
