New serum biochemical markers (Coll 2-1 and Coll 2-1 NO2) for studying oxidative-related type II collagen network degradation in patients with osteoarthritis and rheumatoid arthritis
New serum biochemical markers (Coll 2-1 and Coll 2-1 NO2) for studying oxidative-related type II collagen network degradation in patients with osteoarthritis and rheumatoid arthritis.pdf
[en] Objective: Protein nitration is a prominent feature of inflammatory processes in the joint. We have developed immunoassays specific for a peptide of the alpha-helical region of type II collagen (108)HRGYPGLDG(116) (Coll 2-1) and its nitrated form (108)HRGY(NO2)PGLDG(116) (Coll 2-1 NO2) in biological fluids. Design: Coll 2-1 and Coll 2-1 NO2 peptides were injected into rabbits. Two antisera (D3 and D37) were selected for their specificity and affinity and used to develop specific immunoassays. Coll 2-1 and Coll 2-1 NO2 were measured in sera of 242 healthy subjects (N), 67 patients with primary knee osteoarthritis (OA) and 19 patients with rheumatoid arthritis (RA). Results: In healthy subjects, Coll 2-1 and Coll 2-1 NO2 concentrations were 125.13 +/- 3.71 nM and 0.16 +/- 0.08 nM, respectively. In OA and RA, Coll 2-1 and Coll 2-1 NO2 serum levels were found to be significantly increased compared to controls of the same range of age (Coll 2-1: OA: 200.80 +/- 8.98 nM, RA: 172.30 +/- 19.05 nM, normal: 126.60 +/- 6.70 nM and Coll 2-1 NO2: OA: 0.26 +/- 0.02, RA: 0.38 +/- 0.05, normal: 0.12 +/- 0.01 nM). Coll 2-1 NO2 levels were significantly more elevated in RA than in OA patients (P < 0.05). As a consequence, the ratio Coll 2-1 NO2/Coll 2-1 was 1.6 times higher in RA than in OA subjects. No relationship was found between the radiological OA severity and the levels of Coll 2-1 and Coll 2-1 NO2 in serum. Coll 2-1 NO2, but not Coll 2-1, was correlated with C-reactive protein in the sera of OA and RA patients. Conclusions: The determination of both Coll 2-1 and Coll 2-1 NO2 in serum of arthritic patients seems to be a promising useful tool for the detection of oxidative-related cartilage degradation episode. Further, these markers could be helpful for monitoring the effects of anti-inflammatory or antioxidant drugs on cartilage degradation. (c) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Disciplines :
Orthopedics, rehabilitation & sports medicine Rheumatology
Author, co-author :
Deberg, Michelle ; Université de Liège - ULiège > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Labasse, Alain ; Centre Hospitalier Universitaire de Liège - CHU > Médecine de l'appareil locomoteur
Christgau, S.
Cloos, P.
Bang Henriksen, D.
Chapelle, Jean-Paul ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Zegels, Brigitte ; Université de Liège - ULiège > Département des sciences de la santé publique > Epidémiologie et santé publique
Reginster, Jean-Yves ; Université de Liège - ULiège > Département des sciences de la santé publique > Epidémiologie et santé publique
Henrotin, Yves ; Université de Liège - ULiège > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Language :
English
Title :
New serum biochemical markers (Coll 2-1 and Coll 2-1 NO2) for studying oxidative-related type II collagen network degradation in patients with osteoarthritis and rheumatoid arthritis
A. Hinek A. Reiner A.R. Poole The calcification of cartilage matrix in chondrocyte culture: Studies of the C-propeptide of type II collagen (chondrocalcin) J Cell Biol 104 1987 1435-1441
B. Mansson D. Carey M. Alini M. Ionescu L.C. Rosenberg A.R. Poole et al. Cartilage and bone metabolism in rheumatoid arthritis. Differences between rapid and slow progression of disease identified by serum markers of cartilage metabolism J Clin Invest 95 1995 1071-1077
F. Nelson L. Dahlberg S. Laverty A. Reiner I. Pidoux M. Ionescu et al. Evidence for altered synthesis of type II collagen in patients with osteoarthritis J Clin Invest 102 1998 2115-2125
P. Garnero X. Ayral J.C. Rousseau S. Christgau L.J. Sandell M. Dougados et al. Uncoupling of type II collagen synthesis and degradation predicts progression of joint damage in patients with knee osteoarthritis Arthritis Rheum 46 2002 2613-2624
A.P. Hollander T.F. Heathfiled C. Webber Y. Iwata R. Bourne C. Rorabeck et al. Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay J Clin Invest 93 1994 1722-1732
R.C. Billinghurst L. Dahlberg M. Ionescu A. Reiner R. Bourne C. Rorabeck et al. Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage J Clin Invest 99 1997 1534-1545
L.J. Croucher A.P. Hollander Differential detection of type II collagen N-terminal and C-terminal denaturation epitopes in degrading cartilage J Clin Pathol: Mol Pathol 52 1999 323-331
J.T. Downs C.L. Lane N.B. Nestor T.J. McLellan M.A. Kelly G.A. Karam et al. Analysis of collagenase-cleavage of type II collagen using a neoepitope ELISA J Immunol Methods 247 2001 25-34
S. Christgau P. Garnero C. Fledelius C. Moniz M. Ensig E. Gineyts et al. Collagen type II C-telopeptide fragments as an index of cartilage degradation Bone 29 2001 209-215
A. Fraser U. Fearon R.C. Billinghurst M. Ionescu R. Reece T. Barwick et al. Turnover of type II collagen and Aggrecan in cartilage matrix at the onset of inflammatory arthritis in human Arthritis Rheum 48 2003 3085-3095
P. Garnero M. Piperno E. Gineyts S. Christgau P.D. Delmas E. Vignon Cross sectional evaluation of biochemical markers of bone, cartilage, and synovial tissue metabolism in patients with knee osteoarthritis: relations with disease activity and joint damage Ann Rheum Dis 60 2001 619-626
P. Garnero E. Gineyts S. Christgau B. Finck P.D. Delmas Association of baseline levels of urinary glucosyl-galactosyl-pyridinoline and type II collagen C-telopeptide with progression of joint destruction in patients with early rheumatoid arthritis Arthritis Rheum 46 2002 21-30
A. Van der vliet D. Smith C.A. O'Neill H. Kaur V. Darley-Usmar C.E. Cross et al. Interactions of peroxynitrite with human plasma and its constituents: Oxidative damage and antioxidant depletion Biochem J 303 1994 295-301
D.C. Paik J. Dillon E. Galicia M.D. Tilson The nitrite/collagen reaction: Non-enzymatic nitration as a model system for age-related damage Connect Tissue Res 42 2001 111-122
A.J. Farrell D.R. Blake R.M. Palmer S. Moncada Increased concentrations of nitrite in synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic diseases Ann Rheum Dis 51 1992 1219-1222
T.S. Hiran P.J. Moulton J.T. Hancock Detection of superoxide and NADPH oxidase in porcine articular chondrocytes Free Radic Biol Med 23 1997 736-743
Y.E. Henrotin G.P. Deby-Dupont C. Deby M. De Bruyn M. Lamy P. Franchimont Production of active oxygen species by isolated human chondrocytes Br J Rheumatol 32 1993 562-567
Y.E. Henrotin S.X. Zheng G.P. Deby A.H. Labasse J.M. Crielaard J.Y. Reginster Nitric oxide downregulates interleukin 1 beta (IL-1 beta) stimulated IL-6, IL-8 and prostaglandin E2 production by human chondrocytes J Rheumatol 25 1998 1595-1601
R.F. Loeser C.S. Carlson M. Del Carlo A. Cole Detection of nitrotyrosine in aging and osteoarthritic cartilage: Correlation of oxidative damage with the presence of interleukin-1beta and with chondrocyte resistance to insulin-like growth factor 1 Arthritis Rheum 46 2002 2349-2357
J.P. Pelletier V. Lascau-Coman D. Jovanovic J.C. Fernandes P. Manning J.R. Connor et al. Selective inhibition of inducible nitric oxide synthase in experimental osteoarthritis is associated with reduction in tissue levels of catabolic factors J Rheumatol 26 1999 2002-2014
W.C. Chan P.D. White Fmoc Solid-Phase Peptide Synthesis: A Practical Approach 2000 Oxford University Press Oxford
S. Soinila G.J. Mpitsos J. Soinila Immunohistochemistry of enkephalins: model studies on hapten-carrier conjugates and fixation methods J Histochem Cytochem 40 1992 231-239
S.L. Kohnen A.A. Mouithys-Mickalad G.P. Deby-Dupont C.M. Deby M.L. Lamy A.F. Noels Oxidation of tetrahydrobiopterin by peroxynitrite or oxoferryl species occurs by a radical pathway Free Radic Res 35 2001 709-721
Y. Henrotin C. Bassleer J. Collette B. Nusgens P. Franchimont Radioimmunoassay for human type II collagen J Immunoassay 11 1990 555-578
C. Rosenquist C. Fledelius S. Christgau B.J. Pedersen M. Bonde P. Qvist et al. Serum CrossLaps one step ELISA. First application of monoclonal antibodies for measurement in serum of bone-related degradation products from C-terminal telopeptides of type I collagen Clin Chem 44 1998 2281-2289
M. Mathy-Hartert G.P. Deby-Dupont J.Y. Reginster N. Ayache J.P. Pujol Y.E. Henrotin Regulation by reactive oxygen species of interleukin-1 beta, nitric oxide and prostaglandin E2 production by human chondrocytes Osteoarthritis Cartilage 10 2002 547-555
J.P. Pelletier D.V. Jovanovic V. Lascau-Coman J.C. Fernandes P. Manning J.R. Connors et al. Selective inhibition of inducible nitric oxide synthase reduces progression of experimental osteoarthritis in vivo: possible link with the reduction in chondrocyte apoptosis and caspase-3 level Arthritis Rheum 43 2000 1290-1299
D.R. Eyre J.J. Wu S. Apone A growing family of collagens in articular cartilage: Identification of 5 genetically distinct types J Rheumatol 14 Suppl 1 1987 25-27
M. Rosselli B. Imthurn P.J. Keller E.K. Jackson R.K. Dubey Circulating nitric oxide (nitrite/nitrate) levels in postmenopausal women substituted with 17 beta-estradiol and norethisterone acetate. A two-year follow-up study Hypertension 25 1995 848-853
L.S. Lohmander L.M. Atley T.A. Pietka D.R. Eyre The release of crosslinked peptides from type II collagen into human synovial fluid is increased soon after joint injury and in osteoarthritis Arthritis Rheum 48 2003 3130-3139