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Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli
Rentier-Delrue, Françoise; Swennen, D.; Philippart, Jean-Claude et al.
1989In DNA, 8 (4), p. 271-8
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Keywords :
Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; *Cloning, Molecular/methods; DNA/biosynthesis/genetics; Escherichia coli/*genetics; Fishes/genetics; Growth Hormone/biosynthesis/*genetics; Molecular Sequence Data; Plasmids; RNA, Messenger/biosynthesis; Restriction Mapping; Sequence Homology, Nucleic Acid; Species Specificity; Trout/*genetics
Abstract :
[en] A cDNA library was prepared from poly(A)+RNA extracted from tilapia Oreochromis niloticus anterior pituitaries. The recombinant clones carrying the cDNA sequence of tilapia growth hormone (tiGH) were selected using a fragment of the trout growth hormone (tGH) cDNA as hybridization probe. The nucleotide sequence of the full-length tiGH cDNA was determined. This cDNA encodes a protein of 204 amino acids, including the putative signal peptide of 17 amino acids. Mature tiGH cDNA was inserted in an Escherichia coli expression vector which led to the production of tiGH protein with a yield estimated to be 20% of the total bacterial proteins.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rentier-Delrue, Françoise ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique
Swennen, D.
Philippart, Jean-Claude ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Biologie du comportement - Ethologie et psychologie animale
L'Hoir, C.
Lion, M.
Benrubi, O.;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Martial, Joseph 
Language :
English
Title :
Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli
Publication date :
1989
Journal title :
DNA
ISSN :
0198-0238
Publisher :
Mary Ann Liebert, Inc., New York, United States - New York
Volume :
8
Issue :
4
Pages :
271-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 August 2009

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