Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli

Rentier-Delrue, Françoise; Swennen, D.; Philippart, Jean-Claude; L'Hoir, C.; Lion, M.; Benrubi, O.; Martial, Joseph

doi:10.1089/dna.1.1989.8.271

No full text

Article (Scientific journals)

Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli

Rentier-Delrue, Françoise; Swennen, D.; Philippart, Jean-Claude et al.

1989 • In DNA, 8 (4), p. 271-8

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/20108

DOI
10.1089/dna.1.1989.8.271

PubMed
2670496

Files (0)Send to Details Statistics Bibliography Similar publications

Files

Full Text

No document available.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; *Cloning, Molecular/methods; DNA/biosynthesis/genetics; Escherichia coli/*genetics; Fishes/genetics; Growth Hormone/biosynthesis/*genetics; Molecular Sequence Data; Plasmids; RNA, Messenger/biosynthesis; Restriction Mapping; Sequence Homology, Nucleic Acid; Species Specificity; Trout/*genetics

Abstract :

[en] A cDNA library was prepared from poly(A)+RNA extracted from tilapia Oreochromis niloticus anterior pituitaries. The recombinant clones carrying the cDNA sequence of tilapia growth hormone (tiGH) were selected using a fragment of the trout growth hormone (tGH) cDNA as hybridization probe. The nucleotide sequence of the full-length tiGH cDNA was determined. This cDNA encodes a protein of 204 amino acids, including the putative signal peptide of 17 amino acids. Mature tiGH cDNA was inserted in an Escherichia coli expression vector which led to the production of tiGH protein with a yield estimated to be 20% of the total bacterial proteins.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Rentier-Delrue, Françoise ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique

Swennen, D.

Philippart, Jean-Claude ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Biologie du comportement - Ethologie et psychologie animale

L'Hoir, C.

Lion, M.

Benrubi, O.; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Martial, Joseph

Language :

English

Title :

Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli

Publication date :

1989

Journal title :

DNA

ISSN :

0198-0238

Publisher :

Mary Ann Liebert, Inc., New York, United States - New York

Volume :

Issue :

Pages :

271-8

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 August 2009

Statistics

Number of views

101 (9 by ULiège)

Number of downloads

0 (0 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

AGELLON, L.B., and CHEN, T.T. (1986). Rainbow trout growth hormone: Molecular cloning of cDNA and expression in Escherichia coli. DNA 5, 463–477.
AGELLON, L.B., DAVIES, S.L., CHEN, T.T., and POWERS, D.A. (1988). Structure of a fish (rainbow trout) growth hormone gene and its evolutionary implications. Proc. Natl. Acad. Sci. USA 85, 5136–5140.
BOLTON, J.P., COLLIE, N.L., KAWAUCHI, H., and HIRANO, T. (1987). Osmoregulatory actions of growth hormone in rainbow trout (Salmo gairdneri). J. Endocrinol. 112, 63–68.
CLARKE, W.C., FARMER, S.W., and HARTWELL, K.M. (1977). Effect of teleost pituitary growth hormone on growth of Tilapia mossambica and on growth and seawater adaptation of sockeye salmon (Oncorhynchus nerka). Gen. Comp. Endocrinol. 33, 174–178.
GILL, J.A., SUMPTER, J.P., DONALDSON, E.M., DYE, H.M., SOUZA, L., BERG, T., WYPICH, J., and LANGLEY, K. (1985). Recombinant chicken and bovine growth hormones accelerate growth in aquacultured juvenile pacific salmon oncorhynchus kisutch. Bio/Technology 3, 643–646.
GONZALES-VILLASENOR, L.I., ZHANG, P., CHEN, T.T., and POWERS, D.A. (1988). Molecular cloning and sequencing of coho salmon growth hormone cDNA. Gene 65, 239–246.
GRUNSTEIN, M., and HOGNESS, D.S. (1975). Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA 72, 3961–3965.
KORNFIELD, I.L., RITTE, U., RICHTER, C., and WAHR-MAN, J. (1979). Biochemical and cytological differentiation among Cichild fishes of the sea of Galilee. Evolution 33, 1–14.
KOZAK, M., (1986). Point mutation define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44, 283–292.
KYTE, J., and DOOLITTLE, R.F. (1982). A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105–132.
LAEMMLI, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
LODEMICO, P.T., and McANDREW, S.J. (1982). Eukaryotic ribosome can recognize preproinsulin initiation codons irrespective to their position relative to the 5′ end of mRNA. Nature 299, 221–226.
MILLER, W.L., and EBERHARDT, N.L. (1983). Structure and evolution of the growth hormone gene family. Endocr. Rev. 4, 97–129.
MOFFATT, B.A., and STUDIER, F.W. (1987). T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell 49, 221–227.
MOORE, D.D., WALKER, M.D., DIAMOND, D.J., CONK-LING, M.A., and GOODMAN, H.M. (1982). Structure expression and evolution of growth hormone genes. Rec. Progr. Horm. Res. 38, 197–225.
NICOLL, C.S., MAYER, G.L., and RUSSELL, S.M. (1986). Structural features of prolactins and growth hormones that can be related to their biological properties. Endocr. Rev. 7, 169–203.
NICOLL, C.S., STEINY, S.S., KING, D., NISHIOKA, R.S., MAYER, G.L., EBERHARDT, N.L., BAXTER, J.D., YAMANAKA, M.K., MILLER, J.A., SEILHAMER, J.J., SCHILLING, J.W., and JOHNSON, L.K. (1987). The primary structure of coho salmon growth hormone and its cDNA. Gen. Comp. Endocrinol. 68, 387–399.
OHNO, S., WOLF, U., and ATKIN, N.B. (1968). Evolution from fish to mammals by gene duplication. Hereditas 59, 169–187.
PHILIPPART, J.C., and RUWET, J.C. (1982). Ecology and distribution of tilapias. In The Biology and Culture of Tilapias, R.S.V. Pullin and R.H. Lowe-McConnell, Eds. (ICLARM Conference Proceedings 7, International Center for Living Resources Management, Manilla, The Philipines) pp. 15–59.
RENTIER-DELRUE, F., SWENNEN, D., MERCIER, L., LION, M., BENRUBI, O., and MARTIAL, J.A. (1989a). Molecular cloning and characterization of two forms of trout growth hormone cDNA: Expression and secretion of tGH-II by E. coli. DNA 8, 109–117.
RENTIER-DELRUE, F., SWENNEN, D., PRUNET, P., LION, M., and MARTIAL, J.A. (1989b). Tilapia prolactin: Molecular cloning of two cDNAs and expression in Escherichia coli. DNA 8, 261–270.
RIGBY, P.W.J., DIECKMANN, M., RHODES, C., and BERG, P. (1977). Labeling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J. Mol. Biol. 113, 237–251.
ROSENBERG, A.H., LADE, B.N., CHUI, D.S., LIN, S.W., DUNN, J.J., and STUDIER, F.W. (1987). Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56, 125–135.
SANGER, F., NICKLEN, S., and COULSON, A.R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.
SATO, N., WATANABE, K., MURATA, K., SABAGUCHI, M., KARIYA, Y., KIMURA, S., NONAKA, M., and KIMURA, A. (1988). Molecular cloning and nucleotide sequence of tuna growth hormone cDNA. Biochem. Biophys. Acta 949, 35–42.
SEKINE, S., MIZUKAMI, T., NISHI, T., KUWANA, Y., SAITO, A., SATO, M., ITOH, S., and KAWAUCHI, H. (1985). Cloning and expression of cDNA for salmon growth hormone in Escherichia coli. Proc. Natl. Acad. Sci. USA 82, 4306–4310.
SPECKER, J.L., KING, D.S., RIVAS, R.J., and YOUNG, B.K. (1985). Partial characterization of two prolactins from a cichlid fish. In Prolactin. Basic and Clinical Correlates, vol. 1, R.M. MacLeod, M.O. Thorner, and U. Scapagnigni, Eds. (Fidia Research Series, Liviana Press, Padova, Italy) pp. 427–436.
STUDIER, F.W., and MOFFATT, B.A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 186, 113–130.
THOMAS, P. (1980). Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77, 5201–5205.
TOBWIN, H., STAEHELIN, T., and GORDON, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350–4354.
TSAO, S.G.S., BRUNKA, C.F., and PEARLMAN, R.E. (1983). Hybridization of nucleic acids directly in agarose gels. Anal. Biochem. 131, 365–372.
WATABIKI, N., TANAKA, M., MASUDA, N., YAMAKAWA, M., YONEDA, Y., and NAKAIJMA, K. (1988). cDNA cloning and primary structure of yellow tail (Seriola quinqueradiata) pregrowth hormone. Gen. Comp. Endocrinol. 70, 401–406.
YAMAGUSHI, K., SPECKER, J.L., KING, D.S., YOKOO, Y., NISHIOKA, R.S., HIRANO, T., and BERN, H.A. (1988). Complete amino acid sequence of a pair of fish (tilapia) prolactins tPRLl77 and tPRL188. J. Biol. Chem. 263, 9113–9121.