Transforming growth factor alpha treatment alters intracellular calcium levels in hair cells and protects them from ototoxic damage in vitro.

Staecker, H.; Dazert, S.; Malgrange, Brigitte; Lefèbvre, Philippe; Ryan, A. F.; Van de Water, T. R.

doi:10.1016/S0736-5748(96)00110-4

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Transforming growth factor alpha treatment alters intracellular calcium levels in hair cells and protects them from ototoxic damage in vitro.

Staecker, H.; Dazert, S.; Malgrange, Brigitte et al.

1997 • In International Journal of Developmental Neuroscience, 15 (4-5), p. 553-62

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/200743

DOI
10.1016/S0736-5748(96)00110-4

PubMed
9263032

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Keywords :

Animals; Anti-Bacterial Agents/antagonists & inhibitors/toxicity; Calcium/metabolism; Cells, Cultured; Cilia/metabolism; Fluorescein-5-isothiocyanate; Hair Cells, Auditory/drug effects/metabolism; Hearing Disorders/chemically induced/prevention & control; Histocytochemistry; Image Processing, Computer-Assisted; Immunochemistry; Neomycin/antagonists & inhibitors/toxicity; Organ of Corti/cytology/metabolism; Phalloidine; Rats; Receptor, Epidermal Growth Factor/drug effects/metabolism; Transforming Growth Factor alpha/pharmacology

Abstract :

[en] To determine if transforming growth factor alpha (TGF alpha) pretreatment protects hair cells from aminoglycoside induced injury by modifying their intracellular calcium concentration, we assayed hair cell calcium levels in organ of Corti explants both before and after aminoglycoside (i.e. neomycin, 10(-3) M) exposure either with or without growth factor pretreatment. After TGF alpha (500 ng/ml) treatment, the intracellular calcium level of hair cells showed a five-fold increase as compared to the levels observed in the hair cells of control cultures. After ototoxin exposure, calcium levels in hair cells of control explants showed an increase relative to their baseline levels, while in the presence of growth factors pretreatment, hair cells showed a relative reduction in calcium levels. Pretreatment of organ of Corti explants afforded significant protection of hair cell stereocilia bundle morphology from ototoxic damage when compared to explants exposed to ototoxin alone. This study correlates a rise in hair cell calcium levels with the otoprotection of hair cells by TGF alpha in organ of Corti explants.

Disciplines :

Otolaryngology

Author, co-author :

Staecker, H.

Dazert, S.

Malgrange, Brigitte ; Université de Liège > GIGA - Neurosciences

Lefèbvre, Philippe ; Université de Liège > Département des sciences cliniques > Oto-rhino-laryngologie et audiophonologie

Ryan, A. F.

Van de Water, T. R.

Language :

English

Title :

Transforming growth factor alpha treatment alters intracellular calcium levels in hair cells and protects them from ototoxic damage in vitro.

Publication date :

1997

Journal title :

International Journal of Developmental Neuroscience

ISSN :

0736-5748

eISSN :

1873-474X

Publisher :

Elsevier, Oxford, United Kingdom

Volume :

Issue :

4-5

Pages :

553-62

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 02 August 2016

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