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Abstract :
[en] Cerebellar macroneurones survive and differentiate for at least 10 days in Eagle's minimum essential medium with insulin as the only supplement when cultured either as microexplants or in high-density dissociated cultures, while they do not survive if cultured in low density. The survival is related to the extracellular release of neuronotrophic factor(s). Using a quantitative bioassay of the neuronotrophic effect, it is possible to demonstrate that the effect is concentration dependent. The analysis of the dose-response curves suggests that the neuronotrophic activity is associated with a neuronotoxic activity. The two activities can be segregated using a simple physical method, allowing direct demonstration of the neuronotoxic activity.
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