Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells.

[en] We studied the mtDNA bottleneck in zebrafish to elucidate size, timing, and variation in germline and non-germline cells. Mature zebrafish oocytes contain, on average, 19.0 3 106 mtDNA molecules with high variation between oocytes. During embryo- genesis, the mtDNA copy number decreases to $170 mtDNA molecules per primordial germ cell (PGC), a number similar to that in mammals, and to $50 per non-PGC. These occur at the same developmental stage, implying considerable variation in mtDNA copy number in (non-)PGCs of the same female, dictated by variation in the mature oocyte. The pres- ence of oocytes with low mtDNA numbers, if similar in humans, could explain how (de novo) mutations can reach high mutation loads within a single gener- ation. High mtDNA copy numbers in mature oocytes are established by mtDNA replication during oocyte development. Bottleneck differences between germ- line and non-germline cells, due to early differentia- tion of PGCs, may account for different distribution patterns of familial mutations.

Research Center/Unit :

GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Otten, Auke BC

Theunissen, Tom EJ

Derhaag, Josien G

Lambrichs, Ellen H

Boesten, Iris BW

Winandy, Marie ; Université de Liège - ULiège > GIGA-Management : Plate-forme zebrafish

van Monfoort, Aafke PA

Tarbashevich, Katsiaryna

Raz, Erez

Gerards, Mike

More authors (4 more)

Language :

English

Title :

Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells.

Publication date :

2016

Journal title :

Cell Reports

eISSN :

2211-1247

Publisher :

Elsevier

Pages :

http://dx.doi.org/10.1016/j.celrep.2016.06.023

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 July 2016

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