Article (Scientific journals)
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells.
Otten, Auke BC; Theunissen, Tom EJ; Derhaag, Josien G et al.
2016In Cell Reports, p. 10.1016/j.celrep.2016.06.023
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Abstract :
[en] We studied the mtDNA bottleneck in zebrafish to elucidate size, timing, and variation in germline and non-germline cells. Mature zebrafish oocytes contain, on average, 19.0 3 106 mtDNA molecules with high variation between oocytes. During embryo- genesis, the mtDNA copy number decreases to $170 mtDNA molecules per primordial germ cell (PGC), a number similar to that in mammals, and to $50 per non-PGC. These occur at the same developmental stage, implying considerable variation in mtDNA copy number in (non-)PGCs of the same female, dictated by variation in the mature oocyte. The pres- ence of oocytes with low mtDNA numbers, if similar in humans, could explain how (de novo) mutations can reach high mutation loads within a single gener- ation. High mtDNA copy numbers in mature oocytes are established by mtDNA replication during oocyte development. Bottleneck differences between germ- line and non-germline cells, due to early differentia- tion of PGCs, may account for different distribution patterns of familial mutations.
Research Center/Unit :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Otten, Auke BC
Theunissen, Tom EJ
Derhaag, Josien G
Lambrichs, Ellen H
Boesten, Iris BW
Winandy, Marie  ;  Université de Liège - ULiège > GIGA-Management : Plate-forme zebrafish
van Monfoort, Aafke PA
Tarbashevich, Katsiaryna
Raz, Erez
Gerards, Mike
Vanoevelen, Jo M
van den Bosch, Bianca JC
Muller, Marc  ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Smeets, Hubert JM
More authors (4 more) Less
Language :
English
Title :
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells.
Publication date :
2016
Journal title :
Cell Reports
eISSN :
2211-1247
Publisher :
Elsevier
Pages :
http://dx.doi.org/10.1016/j.celrep.2016.06.023
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 July 2016

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