beef cattle; carcass composition; growth; mapping; quantitative trait loci
Abstract :
[en] This study was conducted to detect quantitative trait loci (QTL) affecting growth and beef carcass fatness traits in an experimental population of Angus and Brahman crossbreds. The three-generation mapping population was generated with 602 progeny from 29 reciprocal backcross and three F-2 full-sib families, and 417 genetic markers were used to produce a sex-averaged map of the 29 autosomes spanning 2,642.5 Kosambi cM. Alternative interval-mapping approaches were applied under line-cross (LC) and random infinite alleles (RA) models to detect QTL segregating between and within breeds. A total of 35 QTL (five with genomewide significant and 30 with suggestive evidence for linkage) were found on 19 chromosomes. One QTL affecting yearling weight was found with genomewide significant evidence for linkage in the interstitial region of bovine autosome (BTA) 1, and an additional 19 QTL were detected with suggestive evidence for linkage under the LC model. Many of these QTL had a dominant (complete or overdominant) mode of gene action, and only a few of the QTL were primarily additive, which reflects the fact that heterosis for growth is known to be appreciable in crosses among Brahman and British breeds. Four QTL affecting growth were detected with genomewide significant evidence for linkage under the RA model on BTA 2 and BTA 6 for birth weight, BTA 5 for yearling weight, and BTA 23 for hot carcass weight. An additional 11 QTL were detected with suggestive evidence for linkage under the RA model. None of the QTL (except for yearling weight on BTA 5) detected under the RA model were found by the LC analyses, suggesting the segregation of alternate alleles within one or both of the parental breeds. Our results reveal the utility of implementing both the LC and RA models to detect dominant QTL and also QTL with similar allele frequency distributions within parental breeds.
Disciplines :
Zoology Agriculture & agronomy
Author, co-author :
Kim, J. J.
Farnir, Frédéric ; Université de Liège - ULiège > Département de productions animales > Biostatistique, économie, sélection animale
Savell, J.
Taylor, J. F.
Language :
English
Title :
Detection of quantitative trait loci for growth and beef carcass fatness traits in a cross between Bos taurus (Angus) and Bos indicus (Brahman) cattle
Alfonso, L., and C. S. Haley. 1998. Power of different F2 schemes for QTL detection in livestock. Anim. Sci. 66:1-8.
Andersson, L. 2001. Genetic dissection of phenotypic diversity in farm animals. Nat. Rev. Genet. 2:130-138.
Andersson, L., C. S. Haley, H. Ellegren, S. A. Knott, M. Johansson, K. Andersson, L. Andersson-Eklund, I. Edfors-Lilja, M. Fredholm, I. Hansson, J. Håkansson, and K. Lundström. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263:1771-1774.
Baker, J. F., C. R. Long, and T. C. Cartwright. 1984. Characterization of cattle of a five breed diallel. V. Breed and heterosis effects on carcass merit. J. Anim. Sci. 59:922-933.
Beever, J. E., H. A. Lewin, W. Barendse, L. Andersson, S. M. Armitage, C. W. Beattie, B. M. Burns, S. K. Davis, S. M. Kappes, B. W. Kirkpatrick, R. Z. Ma, R. A. McGraw, R. T. Stone, and J. F. Taylor. 1996. Report of the first workshop on the genetic map of bovine chromosome 23. Anim. Genet. 27:69-75.
Beever, J. E., Y. Da, and H. A. Lewin. 1997. A genome scan for QTL affecting growth traits in beef cattle. Plant & Animal Genome V Conference, San Diego, CA, p 295.
Blott, S., J.-J. Kim, S. Moisio, A. Schmidt-Kntzel, A. Cornet, P. Berzi, N. Cambisano, C. Ford, B. Grisart, D. Johnson, L. Karim, P. Simon, R. Snell, R. Spelman, J. Wong, J. Vilkki, M. Georges, F. Farnir, and W. Coppieters. 2003. Molecular dissection of a QTL: A phenylalanine to tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition. Genetics 163:253-266.
Boldman, K. G., L. A. Kriese, L. D. Van Vleck, C. P. Van Tassel, and S. D. Kachman. 1995. A manual for use of MTDFREML. A set of programs to obtain estimates of variances and covariances. ARS, USDA, Washington, DC.
Buchanan, D. S., M. K. Nielsen, R. M. Koch, and L. V. Cundiff. 1982. Selection for growth and muscling score in beef cattle. I. Selection applied. J. Anim. Sci. 55:516-525.
Casas, E., S. D. Shackelford, J. W. Keele, R. T. Stone, S. M. Kappes, and M. Koohmaraie. 2000. Quantitative trait loci affecting growth and carcass composition of cattle segregating alternate forms of myostatin. J. Anim. Sci. 78:560-569.
Churchill, G. A., and R. W. Doerge. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138:963-971.
Cockerham, C. C., and Z. Zeng. 1996. Design III with marker loci. Genetics 143:1437-1456.
Comerford, J. W., L. L. Benyshek, J. K. Bertrand, and M. H. Johnson. 1988. Evaluation of performance characteristics in a diallel among Simmental, Limousin, Polled Hereford and Brahman beef cattle. II. Carcass traits. J. Anim. Sci. 66:306-316.
De Koning, D. J., L. L. G. Janss, A. P. Rattink, P. A. M. van Oers, B. J. de Vries, M. A. M. Groenen, J. J. van der Poel, P. N. de Groot, E. W. Brascamp, and J. A. M. van Arendonk. 1999. Detection of quantitative trait loci for backfat thickness and intramuscular fat content in pigs (Sus scrofa). Genetics 152:1679-1690.
De Koning, D. J., B. Harlizius, A. P. Rattink, M. A. M. Groenen, E. W. Brascamp, and J. A. M. van Arendonk. 2001. Detection and characterization of quantitative trait loci for meat quality traits in pigs. J. Anim. Sci. 79:2812-2819.
Elo, K. T., J. Vilkki, D. J. de Koning, R. J. Velmala, and A. V. Mäki-Tanila. 1999. A quantitative trait locus for live weight maps to bovine chromosome 23. Mamm. Genome 10:831-835.
Eshed, Y., and D. Zamir D. 1995. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics 141:1147-1162.
Falconer, D. S., and T. F. C. Mackay. 1996. An Introduction to Quantitative Genetics, Ed. 4. Longman Group, Essex, UK.
Franke, D. E. 1980. Breed and heterosis effects of American Zebu cattle. J. Anim. Sci. 50:1206-1214.
Georges, M., D. Nielsen, M. MacKinnon, A. Mishra, R. Okimoto, A. Pasquino, L. Sargeant, A. Sorensen, M. Steele, X. Zhao, J. E. Womack, and I. Hoeschele. 1995. Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing. Genetics 139:907-920.
Gerbens, F., D. J. Koning, F. L. Harders, T. H. E. Meuwissen, L. L. G. Janss, M. A. M. Groenen, J. H. Veerkamp, J. A. M. Van Arendonk, and M. F. W. te Pas. 2000. The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs. J. Anim. Sci. 78:552-559.
Green, P., K. Falls, and S. Crooks. 1990. CRI-MAP documentation: Version 2.4. Washington University School, St. Louis, MO, USA.
Grignola, F. E., I. Hoeschele, and B. Tier. 1996a. Mapping quantitative trait loci in outcross population via residual maximum likelihood: I. Methodology. Genet. Sel. Evol. 28:479-490.
Grignola, F. E., I. Hoeschele, and B. Tier. 1996b. Mapping quantitative trait loci in outcross population via residual maximum likelihood: II. A simulation study. Genet. Sel. Evol. 28:491-504.
Grosz, M. D., and M. D. MacNeil. 2001. Putative quantitative trait locus affecting birth weight on bovine chromosome 2. J. Anim. Sci. 79:68-72.
Haley, C. S., and S. A. Knott. 1992. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Genetics 132:1211-1222.
Haley, C. S., S. A. Knott, and J.-M. Elsen. 1994. Mapping quantitative trait loci in crosses between outbred lines using least squares. Genetics 136:1195-1207.
Hoeschele, I., F. E. Grignola, Y. I. Li, G. Thaller, P. Uimari, and Q. Zhange. 1998. MQREML, MQAREML, MPLGIB and NQTLGIB: Software for QTL mapping in outcross or complex pedigrees. Proc. 6th World Cong. Genet. Appl. Livest. Prod., Armidale, Australia XXVII:441-442.
Hollick, J. B., and V. L. Chandler. 1998. Epigenetic allelic states of a maize transcriptional regulatory locus exhibit overdominant gene action. Genetics 150:891-897.
Kim, J. J., and J. F. Taylor. 2001. Evaluation of beef carcass and palatability traits and prediction of tenderness in a cross of Bos indicus x Bos taurus cattle. Asian-Aust. J. Anim. Sci. 14:1621-1627.
Knott, S. A., L. Marklund, C. S. Haley, K. Andersson, W. Davies, H. Ellegren, M. Fredholm, I. Hansson, B. Hoyheim, K. Lundström, M. Moller, and L. Andersson. 1998. Multiple marker mapping of quantitative trait loci in a cross between outbred wild boar and Large White pigs. Genetics 149:1069-1080.
Koch, R. M., K. E. Gregory, and L. V. Cundiff. 1974. Selection in beef cattle. I. Selection applied and generation interval. J. Anim. Sci. 39:449-458.
Koots, K. R., J. P. Gibson, C. Smith, and J. W. Wilton. 1994a. Analyses of published genetic parameter estimates for beef production traits. 1. Heritability. Anim. Breed. Abstr. 62:309-338.
Koots, K. R., J. P. Gibson, C. Smith, and J. W. Wilton. 1994b. Analyses of published genetic parameter estimates for beef production traits. 2. Phenotypic and genetic correlations. Anim. Breed. Abstr. 62:825-853.
Lander, E., and L. Kruglyak. 1995. Genetic dissection of complex traits: Guidelines for interpreting and reporting linkage results. Nat. Genet. 11:241-247.
Li, C., J. Basarab, W. M. Snelling, B. Benkel, B. Murdoch, and S. S. Moore. 2002. The identification of common haplotypes on bovine chromosome 5 within commercial lines of Bos taurus and their associations with growth traits. J. Anim. Sci. 80:1187-1194.
Marklund L., P. Nyström, S. Stern, L. Andersson-Eklund, and L. Andersson. 1999. Confirmed quantitative trait loci for fatness and growth on pig chromosome 4. Heredity 82:134-141.
Meuwissen, T. H. E., A. Karlsen, S. Lien, L. Olsaker, and M. E. Goddard. 2002. Fine mapping of a quantitative trait locus for twining rate using combined linkage and linkage disequilibrium mapping. Genetics 161:373-379.
Spelman, R. J., W. Coppieters, L. Karim, J. A. M. van Arendonk, and H. Bovenhuis. 1996. Quantitative trait loci analysis for five milk production traits on chromosome six in the Dutch Holstein-Friesian population. Genetics 144:1799-1808.
Stone, R. T., J. W. Keele, S. D. Shackelford, S. M. Kappes, and M. Koohmaraie. 1999. A primary screen of the bovine genome for quantitative trait loci affecting carcass and growth traits. J. Anim. Sci. 77:1379-1384.
USDA. 1989. Official United States standards for grades of carcass beef. U.S. Department of Agriculture Marketing Service, Washington, DC.
Vargas, C. A., M. A. Elzo, C. C. Chase, Jr., and T. A. Olson. 2000. Genetic parameters and relationships between hip height and weight in Brahman cattle. J. Anim. Sci. 78:3045-3052.
Zhang, Q., D. Boichard, I. Hoeschele, C. Ernst, A. Eggem, B. Murkve, M. Pfister-Genskow, L. A. Witte, F. E. Grignola, P. Uimari, G. Thaller, and M. D. Bishop. 1998. Mapping quantitative trait loci for milk production and health of dairy cattle in a large outbred pedigree. Genetics 149:1959-1973.
