bacteria; eukaryotes; diversity; Antarctica; cyanobacteria; green algae
Abstract :
[en] The bacterial and microeukaryotic biodiversity were studied using pyrosequencing analysis on a 454 GS FLX+ platform
of partial SSU rRNA genes in terrestrial and aquatic habitats of the Sør Rondane Mountains, including soils, on mosses,
endolithic communities, cryoconite holes and supraglacial and subglacial meltwater lenses. This inventory was
complemented with Denaturing Gradient Gel Electrophoresis targeting Chlorophyta and Cyanobacteria. OTUs belonging to
the Rotifera, Chlorophyta, Tardigrada, Ciliophora, Cercozoa, Fungi, Bryophyta, Bacillariophyta, Collembola and Nematodawere present with a relative abundance of at least 0.1% in the eukaryotic communities. Cyanobacteria, Proteobacteria,
Bacteroidetes, Acidobacteria, FBP and Actinobacteria were the most abundant bacterial phyla. Multivariate analyses of the
pyrosequencing data revealed a general lack of differentiation of both eukaryotes and prokaryotes according to habitat type.
However, the bacterial community structure in the aquatic habitats was dominated by the filamentous cyanobacteria
Leptolyngbya and appeared to be significantly different compared with those in dry soils, on mosses, and in endolithic
habitats. A striking feature in all datasets was the detection of a relatively large amount of sequences new to science,
which underscores the need for additional biodiversity assessments in Antarctic inland locations.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Environmental sciences & ecology Microbiology
Author, co-author :
Obbels, Dagmar
Verleyen, Elie
Mano, Marie-José
Namsaraev, Zorigto
Sweetlove, Maxime
Tytgat, Bjorn
FERNANDEZ CARAZO, Rafael ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire génétique moléculaire
De Wever, Aaike
D'hondt, Sofie
Ertz, Damien
Elster, Josef
Sabbe, Koen
Willems, Anne
Wilmotte, Annick ; Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes
Bacterial and eukaryotic biodiversity patterns in terrestrial and aquatic habitats in the 1 Sør Rondane Mountains, Dronning Maud Land, East Antarctica
Publication date :
2016
Journal title :
FEMS Microbiology Ecology
ISSN :
0168-6496
eISSN :
1574-6941
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
92
Pages :
fiw041
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
BELDIVA
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] UGent - Universiteit Gent [BE] Fonds Inbev Baillet Latour
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