Reference : Polyphasic study of Antarctic cyanobacterial strains
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Aquatic sciences & oceanology
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Polyphasic study of Antarctic cyanobacterial strains
Taton, A. [> > > >]
Grubisic, Stana mailto [Université de Liège - ULiège > Services administratifs généraux > Protection et hygiène du travail (SUPHT) >]
Ertz, D. [> > > >]
Hodgson, D. A. [> > > >]
Piccardi, R. [> > > >]
Biondi, N. [> > > >]
Tredici, M. R. [> > > >]
Mainini, M. [> > > >]
Losi, D. [> > > >]
Marinelli, F. [> > > >]
Wilmotte, Annick mailto [Université de Liège - ULiège > Département des sciences de la vie > Enzymologie >]
Journal of Phycology
Blackwell Publishing
Yes (verified by ORBi)
United Kingdom
[en] Antarctic lakes ; bioactive compounds ; cyanobacteria ; microbial mats ; polyphasic characterization ; rRNA operon
[en] We isolated 59 strains of cyanobacteria from the benthic microbial mats of 23 Antarctic lakes, from five locations in two regions, in order to characterize their morphological and genotypic diversity. On the basis of their morphology, the cyanobacteria were assigned to 12 species that included four Antarctic endemic taxa. Sequences of the ribosomal RNA gene were determined for 56 strains. In general, the strains closely related at the 16S rRNA gene level belonged to the same morphospecies. Nevertheless, divergences were observed concerning the diversity in terms of species richness, novelty, and geographical distribution. For the 56 strains, 21 operational taxonomic units (OTUs, defined as groups of partial 16S rRNA gene sequences with more than 97.5% similarity) were found, including nine novel and three exclusively Antarctic OTUs. Sequences of Petalonema cf. involvens and Chondrocystis sp. were determined for the first time. The internally transcribed spacer (ITS) between the 16S and the 23S rRNA genes was sequenced for 33 strains, and similar groupings were observed with the 16S rRNA gene and the ITS, even when the strains were derived from different lakes and regions. In addition, 48 strains were screened for antimicrobial and cytotoxic activities, and 17 strains were bioactive against the gram-positive Staphylococcus aureus, or the fungi Aspergillus fumigatus and Cryptococcus neoformans. The bioactivities were not in coincidence with the phylogenetic relationships, but rather were specific to certain strains.
UE - Union Européenne

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