Polyphasic characterization of 10 selected ecologically relevant filamentous cyanobacterial strains from the South Shetland Islands, Maritime Antarctica
Jancusova, Miroslava; Kovacik, Lubomir; Pereira, Antonio Batistaet al.
2016 • In FEMS Microbiology Ecology, 92 (7), p. 100
[en] The evolutionary relationships of 10 Antarctic cyanobacterial strains of the order Oscillatoriales isolated from King George
and Deception Islands, South Shetland Islands were studied by a polyphasic approach (morphology, 16S rRNA and internal
transcribed spacer sequences). The studied taxa are characteristic of coastal Antarctic biotopes, where they form distinct
populations and ecologically delimited communities. They were isolated from terrestrial habitats: microbial mats in
seepages; crusts on soil, rocks, bones and mosses; mud, sometimes close to bird colonies; and from guano. Based on major
phenotypic features, the strains were divided into four distinct morphotypes: Leptolyngbya borchgrevinkii (A), Leptolyngbya
frigida (B), Microcoleus sp. (C) and Wilmottia murrayi (D). This morphological identification was in agreement with the
phylogenetic relationships. For the first time, the 16S rRNA gene sequence of a strain corresponding to the L. borchgrevinkii
morphotype was determined. Morphotype B is most related to sequences assigned to L. frigida isolated from microbial mats
of coastal lakes in East Antarctica. Morphotype C belongs to a cluster including strains with morphotypes corresponding to
Microcoleus attenuatus, Microcoleus favosus and Microcoleus sp., which are from Antarctica and other continents. Morphotype
D is grouped with sequences assigned to W. murrayi mostly isolated from Antarctica.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology Environmental sciences & ecology
Author, co-author :
Jancusova, Miroslava
Kovacik, Lubomir
Pereira, Antonio Batista
Dusinsky, Roman
Wilmotte, Annick ; Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
Polyphasic characterization of 10 selected ecologically relevant filamentous cyanobacterial strains from the South Shetland Islands, Maritime Antarctica
F.R.S.-FNRS - Fonds de la Recherche Scientifique BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique ERASMUS
Anagnostidis K, Komárek J. Modern approach to the classification system of cyanophytes. 1. Introduction. Arch Hydrobiol 1985;38/39(Suppl. 71):291-302.
Anagnostidis K, Komárek J. Modern approach to the classification system of cyanophytes. 3. Oscillatoriales. Arch Hydrobiol 1988;50/53(Suppl. 80):327-472.
Bartrons M, Catalan J, Casamayor EO. High bacterial diversity in epilithic biofilms of oligotrophic mountain lakes. Microb Ecol 2012;64:860-69.
Bohunická M, Johansen JR, Fučíková K. Tapinothrix clintonii sp. nov. (Pseudanabaenaceae, Cyanobacteria) a new species at the nexus of five genera. Fottea 2011;11:127-40.
Broady PA. A preliminary survey of the terrestrial algae of the Antarctic Peninsula and South Georgia. Br Antarct Surv Bull 1979;48:47-70.
Broady PA. Ecology and taxonomy of the terrestrial algae of the Vestfold Hills. In: Pickard J (ed). Antarctic Oasis. Terrestrial Environments and History of the Vestfold Hills. Sydney: Academic Press, 1986, 165-202.
Broady PA, Garrick R, Anderson G. Culture studies on the morphology of ten strains of Antarctic Oscillatoriaceae (Cyanobacteria). Polar Biol 1984;2:233-44.
Broady PA, Kibblewhite AL. Morphological characterization of Oscillatoriales (Cyanobacteria) from Ross Island and Southern Victoria Land, Antarctica. Ant Sci 1991;3:35-45.
Casamatta DA, Johansen JR, Vis ML et al. Molecular and morphological characterization of ten polar and near-polar strains within the Oscillatoriales (Cyanobacteria). J Phycol 2005;41:421-38.
Comte K, Šabacká M, Carré-Mlouka A et al. Relationships between the Arctic and the Antarctic cyanobacteria; three Phormidium-like strains evaluated by a polyphasic approach. FEMS Microbiol Ecol 2007;59:366-76.
Cuzman OA, Ventura S, Sili C et al. Biodiversity of phototrophic biofilms dwelling on monumental fountains. Microb Ecol 2010;60:81-95.
Elster J. Ecological classification of terrestrial algal communities in Polar environments. In: Beyer L, Bölter M (eds). Geoecology of Antarctic Ice-Free Coastal Landscapes. Ecological Studies 154. Berlin, Heidelberg: Springer, 2002, 303-26.
Feder M, Phoenix V, Haig S et al. Influence of biofilms on heavy metal immobilization in Sustainable urban Drainage Systems (SuDS). Environm Technol 2015;36:1-23.
Fernández-Carazo R, Hodgson DA, Convey P et al. Low cyanobacterial diversity in biotopes of the Transantarctic Mountains and Shackleton Range (80-82°S), Antarctica. FEMS Microbiol Ecol 2011;77:503-17.
Gevers D, Cohan FM, Lawrence JG et al., Opinion: Re-evaluating prokaryotic species. Nat Rev Microbiol 2005;3:733-39.
Hašler P, Dvořák P, Johansen JR et al. Morphological and molecular study of epipelic filamentous genera Phormidium, Microcoleus and Geitlerinema (Oscillatoriales, Cyanophyta/Cyanobacteria). Fottea 2012;12:341-56.
Iteman I, Rippka R, Tandeau de Marsac N et al. Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria. Microbiology 2000;146:1275-86.
Jungblut AD, Hawes I, Mountfort D et al. Diversity within cyanobacterial matcommunities in variable salinity meltwater ponds of McMurdo Ice Shelf, Antarctica. Environm Microbiol 2005;7:519-29.
Kearse M, Moir R, Wilson A et al. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 2012; 28:1647-49.
Komárek J. About endemism of cyanobacteria in freshwater habitats of maritime Antarctica. Algol Stud 2015; 148:15-32.
Komárek J. Cyanobacterial taxonomy: current problems and prospects for the integration of traditional and molecular approaches. Algae 2006;21:349-75.
Komárek J. Phenotype diversity of the cyanobacterial genus Leptolyngbya in the maritime Antarctic. Pol Polar Res 2007;28:211- 31.
Komárek J, Anagnostidis K. Süsswasserflora von Mitteleuropa, Bd 19/2, Cyanoprokaryota, Part 2, Oscillatoriales. Heidelberg: Spektrum Akademische, 2005.
Komárek J, Elster J, Komárek O. Diversity of the cyanobacterial microflora of the northern part of James Ross Island, NW Weddell Sea, Antarctica. Polar Biol 2008;31:853-65.
Komárek J, Kaštovský J, Mareš J et al. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014 using a polyphasic approach. Preslia 2014;86:295-335.
Komárek J, Komárek O. Diversity and ecology of cyanobacterial microflora of Antarctic seepages habitats. Comparison of King George Island, Shetland Islands, and James Ross Island, NW Weddell Sea, Antarctica. In: Seckback J, Oren A (eds). Microbial Mats. Modern and Ancient Microorganisms in Stratified Systems. Dordrecht: Springer, 2010, 517-39.
Komárek J, Komárek O. Diversity of cyanobacteria in seepages of King George Island, maritime Antarctica. In: Huiskes AHL, Gieskes WWC, Rozema J et al. (eds). Antarctic Biology in a Global Context. Leiden: Backhuys Publishers, 2003, 244-50.
Konstantinidis KT, Tiedje JM. Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci U S A 2005;102:2567-72.
Ling HU, Seppelt RD. Non-marine algae and cyanobacteria of the Windmill Islands region, Antarctica with descriptions of two new species. Algol Stud 1998;89:49-62.
Marquardt J, Palinska KA. Genotypic and phenotypic diversity of cyanobacteria assigned to the genus Phormidium (Oscillatoriales) from different habitats and geographical sites. Arch Microbiol 2007;187:397-413.
Palinska KA, Marquardt J. Genotypic and phenotypic analysis of strains assigned to the widespread cyanobacterial morphospecies Phormidium autumnale (Oscillatoriales). Arch Microbiol 2008;189:325-35.
Priscu JC, Fritsen CH, Adams EE et al. Perennial Antarctic lake ice: an oasis for life in a polar desert. Science 1998;280:2095-98.
Rippka R, Deruelles J, Waterbury JB et al. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 1979;111:1-61.
Schloss PD, Westcott SL, Ryabin T et al. Introducing mothur: open-Source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 2009;75:7537-41.
Schmidt SK, Lynch RC, King AJ et al. Phylogeography of microbial phototrophs in the dry valleys of the high Himalayas and Antarctica. Proc Biol Sci 2011;278:702-08.
Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152-55.
Stackebrandt E, Goebel BM. Taxonomic note: a place for DNADNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 1994;44:846-49.
Strunecký O, Elster J, Komárek J. Phylogenetic relationships between geographically separate Phormidium cyanobacteria: is there a link between north and south polar regions? Polar Biol 2010;33:1419-28.
Strunecký O, Elster J, Komárek J. Taxonomic revision of the freshwater cyanobacterium "Phormidium"murrayi =Wilmottiamurrayi. Fottea 2011;11:57-71.
Strunecký O, Komárek J, Johansen J et al. Molecular and morphological criteria for revision of the genus Microcoleus (Oscillatoriales, Cyanobacteria). J Phycol 2013; 49:1167-80.
Tamura K, Stecher G, Peterson D et al. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013; 30:2725-29.
Taton A, Grubisic S, Balthasart P et al. Biogeographical distribution and ecological ranges of benthic cyanobacteria in East Antarctic lakes. FEMS Microbiol Ecol 2006b;57:272-89.
Taton A, Grubisic S, Brambilla E et al. Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): A morphological and molecular approach. Appl Environ Microb 2003;69:5157-69.
Taton A, Grubisic S, Ertz D et al. Polyphasic study of Antarctic cyanobacterial strains. J Phycol 2006a;42:1257-70.
Taton A, Hoffmann L, Wilmotte A. Cyanobacteria in microbial mats of Antarctic lakes (East Antarctica)-a microscopical approoach. Algol Stud 2008;126:173-208.
Van de Peer Y, De Wachter R. Construction of evolutionary distance trees with TREECON forWindows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 1997;13:227-30.
Vincent WF. Cyanobacterial dominance in the polar regions. In: Whitton BA, Potts M (eds). The Ecology of Cyanobacteria. Dordrecht: Kluwer Academic Publishers, 2000, 321-40.
West W, West GS. Freshwater algae. In: British Antarctic Expedition 1907-9. Reports on the Scientific Investigation, Vol. I, Part VII. London: William Heinemann, 1911, 263-98.
Wilmotte A. Molecular evolution and taxonomy of the cyanobacteria. In: Bryant DA (ed.). TheMolecular Biology of Cyanobacteria. Dordrecht: Kluwer Academic Publishers, 1994, 1-25.
