Comparison of ozone-induced effects on lung mechanics and hemodynamics in the rabbit.

hemodynamics; lung mechanics; rabbit lungs; ozone; Acetylcholine/pharmacology; Air Pollutants/toxicity; Airway Resistance/drug effects; Animals; Dose-Response Relationship, Drug; Female; Hemodynamics/drug effects; Histamine/pharmacology; Lung/drug effects; Lung Compliance/drug effects; Male; Ozone/toxicity; Pulmonary Circulation/drug effects; Rabbits; Respiratory Mechanics/drug effects; Substance P/pharmacology

Abstract :

[en] The effects of rabbit exposure to ozone (O3)(0.4 ppm for 4 h) on pulmonary mechanical properties and hemodynamics have been investigated on the isolated perfused lung model. Tracheal pressure, airflow, and tidal volume were measured in order to calculate lung resistance (RL) and dynamic compliance (Cdyn). Using the arterial/venous/double occlusion method, the total pressure gradient (deltaPT) was partitioned into four components (arterial, pre-, postcapillary and venous). Dose-response curves to acetylcholine (ACh), substance P (SP), and histamine were constructed in lungs isolated from rabbits immediately or 48 h after air or O3 exposure O3 induced a significant increase in the baseline value of deltaPt, more markedly 48 h after the exposure. Immediately after the exposure, O3 partly inhibited the ACh-, SP-, and histamine-induced decreases in Cdyn and increases in RL. This inhibitory effect was still in part present 48 h after O3 treatment. In the groups studied immediately after exposure, O3 did not significantly modify the ACh-, SP-, and histamine-induced vasoconstriction. Forty-eight hours after exposure, O3 induced a contractile response to ACh and SP in the arterial segment but decreased the response to histamine. We conclude that O3 can induce direct vascular constriction. Directly, but also 48 h after exposure, O3 can inhibit the ACh-, SP-, and histamine-induced changes in lung mechanical properties. Ozone can also induce some changes in the intensity and in the location of the vascular responses to ACh, SP, and histamine.

Disciplines :

Veterinary medicine & animal health
Pharmacy, pharmacology & toxicology

Author, co-author :

Delaunois, Annie ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Segura, P.

Montano, L. M.

Vargas, M. H.

Ansay, Michel ; Université de Liège - ULiège > Services généraux (Faculté de médecine vétérinaire) > Relations académiques et scientifiques (Méd. vétérinaire)

Gustin, Pascal ; Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie

Language :

English

Title :

Comparison of ozone-induced effects on lung mechanics and hemodynamics in the rabbit.

Publication date :

1998

Journal title :

Toxicology and Applied Pharmacology

ISSN :

0041-008X

eISSN :

1096-0333

Publisher :

Academic Press, New York, United States - New York

Volume :

150

Issue :

Pages :

58-67

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

Fonds de la Recherche Scientifique (Communauté française de Belgique) - FNRS; the European Communities (Grant C11*CT93-0032)

Available on ORBi :

since 25 August 2009

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