No full text
Article (Scientific journals)
High Concentrations of Histamine Stimulate Equine Polymorphonuclear Neutrophils to Produce Reactive Oxygen Species
Benbarek, H.; Mouithys-Mickalad, Ange; Deby-Dupont, G. et al.
1999In Inflammation Research, 48 (11), p. 594-601
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Abstract :
[en] OBJECTIVE AND DESIGN: Because high concentrations of histamine are locally released in inflammation, we investigated the effects of supraphysiological doses of histamine on the production of reactive oxygen species (ROS) by neutrophils. MATERIALS AND METHODS: Isolated equine neutrophils were activated by 10(-4) to 5 x 10(-3) M histamine. The production of ROS and free radicals was estimated by luminol-enhanced chemiluminescence (CL) and electron spin resonance (ESR) with spin trapping technique. In this model of histamine-stimulated neutrophils, we tested the antagonists of H1 and H2 histamine receptors, the role of Ca2+ and Mg2+, the role of staurosporine and pertussis toxin (inhibitors of protein kinase C and proteins G) and the effects of superoxide dismutase, catalase, hydroxyl radical scavengers (phenylalanine and mannitol) and N(G)-monomethyl-L-arginine (L-NMMA), inhibitor of NO-synthase. RESULTS: Histamine (from 10(-5) to 10(-3) M) stimulated neutrophils to produce CL and ESR signals characterized by spin adducts of superoxide anion and/or hydroxyl radicals. The CL response was inhibited by 10(-4) and 10(-3) M H1 receptor antagonists (promethazine, pyrilamine, and diphenhydramine), by Ca2+ and Mg2+ depletion and by 10 nmoles staurosporine. CL was partially inhibited by pertussis toxin (4 microg/ mL). The ESR signals were practically suppressed by pyrilamine (an H1 receptor antagonist) and superoxide dismutase, and partially inhibited by catalase, hydroxyl radical scavengers and L-NMMA (respectively 59, +/- 30% and 68% inhibition). CONCLUSIONS: High concentrations of histamine stimulated the neutrophils to product ROS and free radicals via H1 receptors and the NADPH-oxidase pathway.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Benbarek, H.
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Deby-Dupont, G.
Deby, Christiane ;  Centre Hospitalier Universitaire de Liège - CHU > Administration des patients - Admission des hospitalisés
Grulke, Sigrid  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Nemmar, A.
Lamy, Maurice ;  Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
High Concentrations of Histamine Stimulate Equine Polymorphonuclear Neutrophils to Produce Reactive Oxygen Species
Publication date :
November 1999
Journal title :
Inflammation Research
ISSN :
1023-3830
eISSN :
1420-908X
Publisher :
Birkhauser Verlag, Switzerland
Volume :
48
Issue :
11
Pages :
594-601
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 May 2016

Statistics


Number of views
48 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
31
Scopus citations®
without self-citations
27
OpenCitations
 
23

Bibliography


Similar publications



Contact ORBi