[en] Aflatoxin M1 (AFM1), one of the most toxic mycotoxins, imposes serious health hazards. AFM1 had previously been classified as a group 2B carcinogen1 and has been classified as a group 1 carcinogen by the International Agency for Research on Cancer (IARC) of the World Health Organization (WHO).2 Determination of AFM1 thus plays an important role for quality control of food safety. In this work, a sensitive and reliable aptasensor was developed for the detection of AFM1. The immobilization of aptamer through a strong interaction with biotin–streptavidin was used as a molecular recognition element, and its complementary ssDNA was employed as the template for a real-time quantitative polymerase chain reaction (RT-qPCR) amplification. Under optimized assay conditions, a linear relationship (ranging from 1.0×10-4 to 1.0 µg L-1) was achieved with a limit of detection (LOD) down to 0.03 ng L-1. In addition, the aptasensor developed here exhibits high selectivity for AFM1 over other mycotoxins and small effects from cross-reaction with structural analogs. The method proposed here has been successfully applied to quantitative determination of AFM1 in infant rice cereal and infant milk powder samples. Results demonstrated that the current approach is potentially useful for food safety analysis, and it could be extended to a large number of targets.
Disciplines :
Chemistry
Author, co-author :
Guo, Xiaodong
Wen, Fang
Zheng, Nan
Li, Songli
Fauconnier, Marie-Laure ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie générale et organique
Wang, Jiaqi
Language :
English
Title :
A qPCR aptasensor for sensitive detection of aflatoxin M1
Publication date :
2016
Journal title :
Analytical and Bioanalytical Chemistry
ISSN :
1618-2642
eISSN :
1618-2650
Publisher :
Springer Science & Business Media B.V., Berlin, Germany
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
International Agency for Research on Cancer (IARC). Some naturally occurring substances: Food items and constituents heterocyclic aromatic amines and mycotoxins. IARC monographs on the evaluation of carcinogenic risk to humans. 1993;56:489–51.
International Agency for Research on Cancer (IARC). Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. 2002;82:171–6.
Pei SC, Zhang YY, Eremin SA, Lee WJ. Detection of aflatoxin M1 in milk products from China by ELISA using monoclonal antibodies. Food Control. 2009;20(12):1080–5. doi:10.1016/j.foodcont.2009.02.004.
Anfossi L, Baggiani C, Giovannoli C, Biagioli F, D’Arco G, Giraudi G. Optimization of a lateral flow immunoassay for the ultrasensitive detection of aflatoxin M1 in milk. Anal Chim Acta. 2013;772:75–80. doi:10.1016/j.aca.2013.02.020.
Liu D, Huang Y, Wang S, Liu K, Chen M, Xiong Y, et al. A modified lateral flow immunoassay for the detection of trace aflatoxin M1 based on immunomagnetic nanobeads with different antibody concentrations. Food Control. 2015;51:218–24. doi:10.1016/j.foodcont.2014.11.036.
Mao J, Lei S, Liu Y, Xiao D, Fu C, Zhong L, et al. Quantification of aflatoxin M1 in raw milk by a core-shell column on a conventional HPLC with large volume injection and step gradient elution. Food Control. 2015;51:156–62. doi:10.1016/j.foodcont.2014.11.022.
Food and Agriculture Organization of the United Nations. Worldwide regulations for mycotoxins in food and feed in 2003. Food and Nutrition Paper. 2004;81: Rome: FAO.
Hoyos Ossa DE, Hincapié DA, Peñuela GA. Determination of aflatoxin M1 in ice cream samples using immunoaffinity columns and ultra-high performance liquid chromatography coupled to tandem mass spectrometry. Food Control. 2015;56:34–40. doi:10.1016/j.foodcont.2015.03.011.
Commission Recommendation (EC). Setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union. 2006;364:5–24.
Busman M, Bobell JR, Maragos CM. Determination of the aflatoxin M1 (AFM1) from milk by direct analysis in real time–mass spectrometry (DART-MS). Food Control. 2015;47:592–8. doi:10.1016/j.foodcont.2014.08.003.
Wang Y, Liu X, Xiao C, Wang Z, Wang J, Xiao H, et al. HPLC determination of aflatoxin M1 in liquid milk and milk powder using solid phase extraction on OASIS HLB. Food Control. 2012;28(1):131–4. doi:10.1016/j.foodcont.2012.04.037.
Lee D, Lee K-G. Analysis of aflatoxin M1 and M2 in commercial dairy products using high-performance liquid chromatography with a fluorescence detector. Food Control. 2015;50:467–71. doi:10.1016/j.foodcont.2014.09.020.
Pietri A, Fortunati P, Mulazzi A, Bertuzzi T. Enzyme-assisted extraction for the HPLC determination of aflatoxin M1 in cheese. Food Chem. 2016;192:235–41. doi:10.1016/j.foodchem.2015.07.006.
Beltrán E, Ibáñez M, Sancho JV, Cortés MÁ, Yusà V, Hernández F. UHPLC–MS/MS highly sensitive determination of aflatoxins, the aflatoxin metabolite M1 and ochratoxin a in baby food and milk. Food Chem. 2011;126(2):737–44. doi:10.1016/j.foodchem.2010.11.056.
Wang X, Li P. Rapid screening of mycotoxins in liquid milk and milk powder by automated size-exclusion SPE-UPLC-MS/MS and quantification of matrix effects over the whole chromatographic run. Food Chem. 2015;173:897–904. doi:10.1016/j.foodchem.2014.10.056.
Li P, Zhang Q, Zhang W, Zhang J, Chen X, Jiang J, et al. Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut. Food Chem. 2009;115(1):313–7. doi:10.1016/j.foodchem.2008.11.052.
Kav K, Col R, Kaan TK. Detection of aflatoxin M1 levels by ELISA in white-brined Urfa cheese consumed in Turkey. Food Control. 2011;22(12):1883–6. doi:10.1016/j.foodcont.2011.04.030.
Anfossi L, Di Nardo F, Giovannoli C, Passini C, Baggiani C. Enzyme immunoassay for monitoring aflatoxins in eggs. Food Control. 2015;57:115–21. doi:10.1016/j.foodcont.2015.04.013.
Parker CO, Tothill IE. Development of an electrochemical immunosensor for aflatoxin M1 in milk with focus on matrix interference. Biosens Bioelectron. 2009;24(8):2452–7. doi:10.1016/j.bios.2008.12.021.
Bacher G, Pal S, Kanungo L, Bhand S. A label-free silver wire based impedimetric immunosensor for detection of aflatoxin M1 in milk. Sensors Actuators B. 2012;168:223–30. doi:10.1016/j.snb.2012.04.012.
Vdovenko MM, Lu CC, Yu FY, Sakharov IY. Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin M1 in milk. Food Chem. 2014;158:310–4. doi:10.1016/j.foodchem.2014.02.128.
Cruz-Aguado JA, Penner G. Determination of ochratoxin a with a DNA aptamer. J Agric Food Chem. 2008;56(22):10456–61. doi:10.1021/jf801957h.
Barthelmebs L, Hayat A, Limiadi AW, Marty J-L, Noguer T. Electrochemical DNA aptamer-based biosensor for OTA detection, using superparamagnetic nanoparticles. Sensors Actuators B. 2011;156(2):932–7. doi:10.1016/j.snb.2011.03.008.
Bonel L, Vidal JC, Duato P, Castillo JR. An electrochemical competitive biosensor for ochratoxin a based on a DNA biotinylated aptamer. Biosens Bioelectron. 2011;26(7):3254–9. doi:10.1016/j.bios.2010.12.036.
Guo X, Wen F, Zheng N, Luo Q, Wang H, Wang H, et al. Development of an ultrasensitive aptasensor for the detection of aflatoxin B1. Biosens Bioelectron. 2014;56:340–4. doi:10.1016/j.bios.2014.01.045.
Shim WB, Kim MJ, Mun H, Kim MG. An aptamer-based dipstick assay for the rapid and simple detection of aflatoxin B1. Biosens Bioelectron. 2014;62:288–94. doi:10.1016/j.bios.2014.06.059.
Wang R, Xiang Y, Zhou X, Liu L-H, Shi H. A reusable aptamer-based evanescent wave all-fiber biosensor for highly sensitive detection of ochratoxin A. Biosens Bioelectron. 2015;66:11–8. doi:10.1016/j.bios.2014.10.079.
Zhao Q, Geng X, Wang H. Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer. Anal Bioanal Chem. 2013;405(19):6281–6. doi:10.1007/s00216-013-7047-2.
De Girolamo A, Le L, Penner G, Schena R, Visconti A. Analytical performances of a DNA-ligand system using time-resolved fluorescence for the determination of ochratoxin A in wheat. Anal Bioanal Chem. 2012;403(9):2627–34. doi:10.1007/s00216-012-6076-6.
Nguyen BH, Tran LD, Do QP, Nguyen HL, Tran NH, Nguyen PX. Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor. Mater Sci Eng C Mater Biol Appl. 2013;33(4):2229–34. doi:10.1016/j.msec.2013.01.044.
Istamboulie G, Paniel N, Zara L, Granados LR, Barthelmebs L, Noguer T. Development of an impedimetric aptasensor for the determination of aflatoxin M1 in milk. Talanta. 2016;146:464–9. doi:10.1016/j.talanta.2015.09.012.
Malhotra S, Pandey AK, Rajput YS, Sharma R. Selection of aptamers for aflatoxin M1 and their characterization. J Mol Recognit. 2014;27(8):493–500. doi:10.1002/jmr.2370.
Babu D, Muriana PM. Immunomagnetic bead-based recovery and real time quantitative PCR (RT iq-PCR) for sensitive quantification of aflatoxin B(1). J Microbiol Methods. 2011;86(2):188–94. doi:10.1016/j.mimet.2011.05.002.
Cavaliere C, Foglia P, Pastorini E, Samperi R, Lagana A. Liquid chromatography/tandem mass spectrometric confirmatory method for determining aflatoxin M1 in cow milk: comparison between electrospray and atmospheric pressure photoionization sources. J Chromatogr A. 2006;1101(1–2):69–78. doi:10.1016/j.chroma.2005.09.060.
Cucci C, Mignani AG, Dall’Asta C, Pela R, Dossena A. A portable fluorometer for the rapid screening of M1 aflatoxin. Sensors Actuators B. 2007;126(2):467–72. doi:10.1016/j.snb.2007.03.036.
Neagu D, Perrino S, Micheli L, Palleschi G, Moscone D. Aflatoxin M1 determination and stability study in milk samples using a screen-printed 96-well electrochemical microplate. Int Dairy J. 2009;19(12):753–8. doi:10.1016/j.idairyj.2009.06.004.
Dinckaya E, Kinik O, Sezginturk MK, Altug C, Akkoca A. Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles. Biosens Bioelectron. 2011;26(9):3806–11. doi:10.1016/j.bios.2011.02.038.
Larou E, Yiakoumettis I, Kaltsas G, Petropoulos A, Skandamis P, Kintzios S. High throughput cellular biosensor for the ultra-sensitive, ultra-rapid detection of aflatoxin M1. Food Control. 2013;29(1):208–12. doi:10.1016/j.foodcont.2012.06.012.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
