Reference : Unraveling the complexity of the interactions of DNA nucleotides with gold by single ...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/196959
Unraveling the complexity of the interactions of DNA nucleotides with gold by single molecule force spectroscopy
English
Bano, Fouzia [Université de Liège > > > >]
Sluysmans, Damien mailto [Université de Liège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires >]
Wislez, Arnaud mailto [Université de Liège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires >]
Duwez, Anne-Sophie mailto [Université de Liège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires >]
2015
Nanoscale
RSC Publishing
7
19528–19533
Yes (verified by ORBi)
International
2040-3364
2040-3372
Cambridge
United Kingdom
[en] AFM ; DNA
[en] Addressing the effect of different environmental factors on the adsorption of DNA to solid supports is critical for the development of robust miniaturized devices for applications ranging from biosensors to next generation molecular technology. Most of the time, thiol-based chemistry is used to anchor DNA on gold – a substrate commonly used in nanotechnology – and little is known about the direct interaction between DNA and gold. So far there have been no systematic studies on the direct adsorption behavior of the deoxyribonucleotides (i.e., a nitrogenous base, a deoxyribose sugar, and a phosphate group) and on the factors that govern the DNA–gold bond strength. Here, using single molecule force spectroscopy, we investigated the interaction of the four individual nucleotides, adenine, guanine, cytosine, and thymine, with gold. Experiments were performed in three salinity conditions and two surface dwell times to reveal the factors that influence nucleotide–Au bond strength. Force data show that, at physiological ionic strength, adenine–Au interactions are stronger, asymmetrical and independent of surface dwell time as compared to cytosine–Au and guanine–Au interactions. We suggest that in these conditions only adenine is able to chemisorb on gold. A decrease of the ionic strength significantly increases the bond strength for all nucleotides. We show that moderate ionic strength along with longer surface dwell period suggest weak chemisorption also for cytosine and guanine.
http://hdl.handle.net/2268/196959
10.1039/C5NR05695K
http://pubs.rsc.org/en/content/articlelanding/2015/nr/c5nr05695k/unauth#!divAbstract

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