Reference : Absence of selenium protection against methylmercury toxicity in harbour seal leucocy...
Scientific journals : Article
Life sciences : Aquatic sciences & oceanology
Life sciences : Environmental sciences & ecology
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro
Das, Krishna mailto [Université de Liège > Département de Biologie, Ecologie et Evolution > Océanographie biologique >]
Dupont, Aurélie []
De Pauw-Gillet, Marie-Claire mailto [Université de Liège > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Debier, Cathy []
Siebert, Ursula []
Marine Pollution Bulletin
Yes (verified by ORBi)
United Kingdom
[en] marine mammals ; pollution ; mercury ; selenium ; immune system
[en] Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 µM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 µM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential.
To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposure with no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea.
Centre Interfacultaire de Recherches en Océanologie - MARE
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA

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