Article (Scientific journals)
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro
Das, Krishna; Dupont, Aurélie; De Pauw-Gillet, Marie-Claire et al.
2016In Marine Pollution Bulletin, 108, p. 70-76
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Keywords :
marine mammals; pollution; mercury; selenium; immune system
Abstract :
[en] Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 µM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 µM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential. To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposure with no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea.
Research Center/Unit :
MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
Disciplines :
Aquatic sciences & oceanology
Environmental sciences & ecology
Author, co-author :
Das, Krishna  ;  Université de Liège > Département de Biologie, Ecologie et Evolution > Océanographie biologique
Dupont, Aurélie
De Pauw-Gillet, Marie-Claire ;  Université de Liège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Debier, Cathy
Siebert, Ursula
Language :
English
Title :
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro
Publication date :
2016
Journal title :
Marine Pollution Bulletin
ISSN :
0025-326X
eISSN :
1879-3363
Publisher :
Pergamon, Oxford, United Kingdom
Volume :
108
Pages :
70-76
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
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