Article (Scientific journals)
Hydrocarbon biostimulation and bioaugmentation in organic carbon and clay-rich soils
Masy, Thibaut; Demanèche, Sandrine; Tromme, Olivier et al.
2016In Soil Biology and Biochemistry, 99, p. 66-74
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Keywords :
Bioremediation; Rhodococcus erythropolis; Hydrocarbons; Oxitop; qPCR; 16S-rRNA gene sequencing
Abstract :
[en] Hydrocarbon-contaminated organic carbon-rich clayey soils are challenging for bioremediation stakeholders since the pollutant is heterogeneously distributed and poorly bioavailable due to its strong adsorption on clay and organic particles. In addition, biodegradation rates are restricted by limited diffusion of oxygen and nutrients to hydrocarbon-degrading aerobes. This study assessed the benefits of bioaugmentation with the strain Rhodococcus erythropolis T902.1 versus those from biostimulation and anaerobic natural attenuation in terms of hydrocarbon (HC) degradation efficiency and changes in the bacterial community structure in a diesel-polluted clay-rich soil. Three soil samples with a similar total organic content but with a different HC concentration (0.2, 1.0 and 6.5 g/kg) were compared in a microcosm experiment. Despite a limitation in oxygen transfer, R. erythropolis T902.1 enhanced a greater HC degradation compared to the biostimulation treatment. However, this advantage decreased with time as the proportion of Rhodococci declined from 25% initially to 1% of the global community after 80 days of treatment. Similarly, the alkB gene proportion in bioaugmented soils decreased to levels close to those of biostimulated soils. Consequently, further engineering was suggested to improve the resilience of the inoculum to ensure its long-term presence and activity in such polluted environments.
Disciplines :
Microbiology
Environmental sciences & ecology
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Masy, Thibaut ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Demanèche, Sandrine;  Université de Lyon - Ecole Centrale de Lyon > Laboratoire Ampère - UMR CNRS 5005 > Groupe de Génomique Microbienne Environnementale
Tromme, Olivier;  Sanifox SPRL
Thonart, Philippe ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Jacques, Philippe ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Hiligsmann, Serge ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Vogel, Timothy M.;  Université de Lyon - Ecole Centrale de Lyon > Laboratoire Ampère - UMR CNRS 5005 > Groupe de Génomique Microbienne Environnementale
Language :
English
Title :
Hydrocarbon biostimulation and bioaugmentation in organic carbon and clay-rich soils
Publication date :
August 2016
Journal title :
Soil Biology and Biochemistry
ISSN :
0038-0717
eISSN :
1879-3428
Publisher :
Elsevier
Volume :
99
Pages :
66-74
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 May 2016

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