Characterization of brown streak virus-resistant cassava.

Anjanappa, Ravi B.; Mehta, Devang; Maruthi, M. N.; Kanju, Edward; Gruissem, Wilhelm; Vanderschuren, Hervé

doi:10.1094/MPMI-01-16-0027-R

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Characterization of brown streak virus-resistant cassava.

Anjanappa, Ravi B.; Mehta, Devang; Maruthi, M. N. et al.

2016 • In Molecular Plant-Microbe Interactions

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https://hdl.handle.net/2268/195837

DOI
10.1094/MPMI-01-16-0027-R

PubMed
27070326

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Keywords :

virus; cassava; crop; resistance

Abstract :

[en] Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies as the disease is progressing westwards to new geographical areas. A stringent infection method based on top cleft grafting combined with precise virus titer quantitation was utilized to screen fourteen cassava cultivars and elite breeding lines. When inoculated with mixed infections of Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the scions of elite breeding lines KBH 2006/18 and KBH 2006/26 remained symptom-free during a 16-week period of virus graft inoculation, while susceptible varieties displayed typical CBSD infection symptoms at 4 weeks after grafting. The identified CBSD resistance was stable under the co-inoculation of CBSV, UCBSV with cassava geminiviruses (CGMs). Double grafting experiments revealed that transmission of CBSV and UCBSV to CBSD susceptible top scions was delayed when using intermediate scions of elite breeding lines KBH 2006/18 and KBH 2006/26. Nonetheless, comparison of virus systemic movement using scions from KBH2006/18 and a transgenic CBSD resistant 60444 line (60444-Hp9 line) showed that both CBSV and UCBSV move at undetectable levels through the stems. Further, protoplast-based assays of virus titers showed that the replication of CBSVs is inhibited in the resistant line KBH2006/18, suggesting that the identified CBSD resistance is at least partially based on inhibition of virus replication. Our molecular characterization of CBSD resistance in cassava offers a robust virus-host system to further investigate the molecular determinants of CBSD resistance.

Disciplines :

Genetics & genetic processes

Author, co-author :

Anjanappa, Ravi B.

Mehta, Devang

Maruthi, M. N.

Kanju, Edward

Gruissem, Wilhelm

Vanderschuren, Hervé ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biologie végétale

Language :

English

Title :

Characterization of brown streak virus-resistant cassava.

Publication date :

2016

Journal title :

Molecular Plant-Microbe Interactions

ISSN :

0894-0282

eISSN :

1943-7706

Publisher :

American Phytopathological Society, United States - Minnesota

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 April 2016

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