The complementary strand of the human T-cell leukemia virus type 1 RNA genome encodes a bZIP transcription factor that down-regulates viral transcription.

Gaudray, Gilles; Gachon, Frederic; Basbous, Jihane; Biard-Piechaczyk, Martine; Devaux, Christian; Mesnard, Jean-Michel

doi:10.1128/JVI.76.24.12813-12822.2002

Article (Scientific journals)

The complementary strand of the human T-cell leukemia virus type 1 RNA genome encodes a bZIP transcription factor that down-regulates viral transcription.

Gaudray, Gilles; Gachon, Frederic; Basbous, Jihane et al.

2002 • In Journal of Virology, 76 (24), p. 12813-22

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https://hdl.handle.net/2268/19579

DOI
10.1128/JVI.76.24.12813-12822.2002

PubMed
12438606

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Keywords :

Activating Transcription Factor 4; Amino Acid Sequence; Animals; Basic-Leucine Zipper Transcription Factors; COS Cells; DNA-Binding Proteins/chemistry/genetics; Down-Regulation; G-Box Binding Factors; Gene Expression Regulation, Viral; Human T-lymphotropic virus 1/genetics; Molecular Sequence Data; RNA, Complementary/genetics; RNA, Viral/genetics; Transcription Factors/chemistry/genetics/metabolism; Transcription, Genetic

Abstract :

[en] The RNA genome of the human T-cell leukemia virus type 1 (HTLV-1) codes for proteins involved in infectivity, replication, and transformation. We report in this study the characterization of a novel viral protein encoded by the complementary strand of the HTLV-1 RNA genome. This protein, designated HBZ (for HTLV-1 bZIP factor), contains a N-terminal transcriptional activation domain and a leucine zipper motif in its C terminus. We show here that HBZ is able to interact with the bZIP transcription factor CREB-2 (also called ATF-4), known to activate the HTLV-1 transcription by recruiting the viral trans-activator Tax on the Tax-responsive elements (TxREs). However, we demonstrate that the HBZ/CREB-2 heterodimers are no more able to bind to the TxRE and cyclic AMP response element sites. Taking these findings together, the functional inactivation of CREB-2 by HBZ is suggested to contribute to regulation of the HTLV-1 transcription. Moreover, the characterization of a minus-strand gene protein encoded by HTLV-1 has never been reported until now.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Gaudray, Gilles ; Université de Liège - ULiège > GIGA-Management : Plate-forme SPF

Gachon, Frederic

Basbous, Jihane

Biard-Piechaczyk, Martine

Devaux, Christian

Mesnard, Jean-Michel

Language :

English

Title :

The complementary strand of the human T-cell leukemia virus type 1 RNA genome encodes a bZIP transcription factor that down-regulates viral transcription.

Publication date :

2002

Journal title :

Journal of Virology

ISSN :

0022-538X

eISSN :

1098-5514

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

12813-22

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 24 August 2009

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373

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349

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