[fr] Le but de cette revue est de décrire les techniques de congélation de la semence équine. Un rappel résumera la formation du spermatozoïde dans le testicule, son anatomie et sa physiologie en insistant sur les structures lésées pendant le processus de congélation et décongélation. Les techniques de congélation du sperme seront ensuite résumées dans différentes espèces et comparées avec celles utilisées pour la semence équine. Les critères actuels de qualité seront définis pour le sperme avant et après congélation en insistant sur la mobilité totale, progressive et les différentes définitions de ces concepts dans la littérature. Les autres méthodes d’analyse comme la cytométrie de flux seront présentées ainsi que leurs intérêts cliniques. Les prochains développements et progrès dans le domaine de la congélation seront enfin envisagés : la prédiction de la qualité du sperme congelé à partir du sperme frais, l’amélioration des dilueurs et la lutte contre les formes activées de l’oxygène.
Disciplines :
Animal production & animal husbandry Veterinary medicine & animal health Biotechnology
Author, co-author :
Ponthier, Jérôme ; Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Anesthésiologie gén. et pathologie chirurg. des grds animaux
van den Berghe, Femke
Parrilla Hernandez, Sonia ; Université de Liège > Département des sciences fonctionnelles (DSF) > Physiologie
Hanzen, Christian ; Université de Liège > Département clinique des animaux de production (DCP) > Thériogenologie des animaux de production
Deleuze, Stefan ; Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Obstét. et path. de la reprod. des anim. de comp. et équidés
Language :
French
Title :
Congélation de sperme dans l'espèce équine: état des lieux et perspectives
Alternative titles :
[en] Equine semen freezing: state of the art and future
AMIRAT L., TAINTURIER D., JEANNEAU L., THORIN C., GERARD O., COURTENS J.L., ANTON M. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl, a commercial egg yolk extender. Theriogenology, 2004, 61, 895-907.
ARIBARG A., SUKCHAROEN N., CHANPRASIT Y., NGEAMVIJAWAT J. Prediction of post-thaw sperm motility and sperm cryosurvival rate using the pre-freeze sperm parameters. J. Med. Assoc. Thai., 1995, 78, 474-480.
BACHTELL N.E., CONAGHAN J., TUREK P.J. The relative viability of human spermatozoa from the vas deferens, epididymis and testis before and after cryopreservation. Hum. Reprod., 1999, 14, 3048-3051.
BAGCHI A., WOODS E.J., CRISTER J.K. Cryopreservation and vitrification: recent advances in fertility preservation technologies. Expert Rev. Med. Devices, 2008, 5, 359-370.
BALL B.A., VO A. Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential. J. Androl., 2001, 22, 1061-1069.
BALL B.A., VO A.T., BAUMBER J. Generation of reactive oxygen species by equine spermatozoa. Am. J. Vet. Res., 2001, 62, 508-515.
BARACALDO M.I. Steps for freezing bovine semen: from semen collection to the liquid nitrogen tank. [en ligne] (2007) Adresse URL: http://www.uesc.br/cursos/pos_graduacao/mestrado/animal/bibliografia2007/baracaldo_et_at_2007.pdf, consulté le 02/04/2014.
BARTHELEMY C., ROYERE D., HAMMAHAH S., LEBOS C., THARANNE M.J., LANSAC J. Ultrastructural changes in membranes and acrosome of human sperm during cryopreservation. Arch. Androl., 1990, 25, 29-40.
BAUMBER J., BALL B.A., GRAVANCE C.G., MEDINA V., DAVIES-MOREL M.C. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. J. Androl., 2000, 21, 895-902.
BAUMBER J., BALL B.A., LINFOR J.J. Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. Am. J. Vet. Res., 2005, 66, 772-779.
BAUMBER J., BALL B.A., LINFOR J.J., MEYERS S.A. Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa. J. Androl., 2003, 24, 621-628.
BECCAGLIA M., ANASTASI P., CHIGIONI S., LUVONI G.C. Tris-lecithin extender supplemented with antioxidant catalase for chilling of canine semen. Reprod. Domest. Anim., 2009, 44, 345-349.
BITTMAR A., KOSINIAK K. The role of selected biochemical components of equine seminal plasma in determining suitability for deep-freezing. Arch. Vet. Pol., 1992, 32, 17-29.
BJORNDAHL L., SODERLUND I., KVIST U. Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment. Hum. Reprod., 2003, 18, 813-816.
BLOM E., BIRCH-ANDERSEN A. The ultrastructure of a characteristic spermhead-defect in the boar: the SME-defect. Andrologia, 1975, 7, 199-209.
BLOTTNER S., WARNKE C., TUCHSCHERER A., HEINEN V., TORNER H. Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season. Anim. Reprod. Sci., 2001, 65, 75-88.
BOERKE A., TSAI P.S., GARCLAGIL N., BREWIS I.A., GADELLA B.M. Capacitation-dependent reorganization of microdomains in the apical sperm head plasma membrane: Functional relationship with zona binding and the zona-induced acrosome reaction. Theriogenology, 2008, 70, 1188-1196.
BOLLWEIN H., FUCHS I., KOESS C. Interrelationship between plasma membrane integrity, mitochondrial membrane potential and DNA fragmentation in cryopreserved bovine spermatozoa. Reprod. Domest. Anim., 2008, 43, 189-195.
BRANCO C.S., GARCEZ M.E., PASQUALOTTO F.F., ERDTMAN B., SALVADOR M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology, 2010, 60, 235-237.
BRAUN J., SAKAI M., HOCHI S., OGURI N. Preservation of ejaculated and epididymal stallion spermatozoa by cooling and freezing. Theriogenology, 1994, 41, 809-818.
BRINSKO S.P., BLANCHARD T.L., RIGBY S.L., LOVE C.C., VARNER D.D. Effects of dead spermatozoa on motion characteristics and membrane integrity of live spermatozoa in fresh and cooled-stored equine semen. Theriogenology, 2003, 59, 735-742.
BREININGER E., BEORLEGUI N.B., O'FLAHERTY C.M., BECONI M.T. Alpha-tocopherol improves bichemical and dynamic parameter in cryopreserved boar semen. Theriogenology, 2005, 63, 2126-2135.
BROTHERTON J. Cryopreservation of human semen. Arch. Androl., 1990, 25, 181-195.
BUCAK M.N., SARIOZKAN S., TUNCER P.B., SAKIN F., ATESSAHIN A., KULAKSIZ R., CEVIK M. The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Res., 2010, 89, 24-30.
CHAN S.Y., CRAFT I.L., CHAN Y.M., LEONG M.K., LEUNG C.K. The hypoosmotic swelling test and cryosurvival of human spermatozoa. Hum. Reprod., 1990, 5, 715-718.
CLULOW J.R., MAXWELL W.M., EVANS G., MORRIS L.H. A comparison of duck and chicken egg yolk for cryopreservation of stallion sperm. Aust. Vet. J., 2007, 85, 232-235.
CORMIER N., SIRARD M.A., BAILEY J.L. Premature capacitation of bovine spermatozoa is initiated by cryopreservation. J. Androl., 1997, 18, 461-468.
CROCKETT E.C., GRAHAM J.K., BRUEMMER J.E., SQUIRES E.L. Effect of cooling of equine spermatozoa before freezing on post-thaw motility: preliminary results. Theriogenology, 2001, 55, 793-803.
CURRY M.R., MILLAR J.D., WATSON P.F. Calculated optimal cooling rates for ram and human sperm cryopreservation fail to conform with empirical observations. Biol. Reprod., 1994, 51, 1014-1021.
DAELS P.F. New technics of artificial insemination in the mare. In: Belgian Equine Practitioners Society (Ed.), Proceedings de la 20e journée d'étude de la Belgian Equine Practitioners Society, Bruxelles, 5 novembre 2003, 2003.
DEFOIN L., GRANADOS A., DONNAY I. Analysing motility parameters on fresh bull semen could help to predict resistance to freezing: a preliminary study. Reprod. Domest. Anim., 2008, 43, 606-611.
DEVIREDDY R.V., SWANLUND D.J., OLIN T., VINCENTE W., TROEDSSON M.H., BISCHOF J.C., ROBERTS K.P. Cryopreservation of equine sperm: optimal cooling rates in the presence and absence of cryoprotective agents determined using differential scanning calorimetry. Biol. Reprod., 2002, 66, 222-231.
EDMOND A.J., TEAGUE S.R., BRINSKO S.P., COMERFORD K.L., WAITE J.A., MANCILL S.S., LOVE C.C., VARNER D.D. Effect of density-gradient centrifugation on quality and recovery rate of equine spermatozoa. Anim. Reprod. Sci., 2008, 107, 318-318.
EGGERT-KRUSEW., ZIMMERMANN K., GEISSLER W., EHRMANN A., BOIT R., STROWITZKI T. Clinical relevance of polymorphonuclear (PMN-) elastase determination in semen and serum during infertility investigation. Int. J. Androl., 2008, 32, 317-329.
EROKHIN A.S., EVTUKH V.P. Effects of Dry Soya-Lecithin (Sternpur PC) on survival of boar spermatozoa after cooling and freezing. Reprod. Domest. Anim., 2007, 42: Suppl 2, 88.
ESTEVES S.C., SPAINE D.M., CEDENHO A.P., SROUGI M. Effects of the technique of cryopreservation and dilution/centrifugation after thawing on the motility and vitality of spermatozoa of oligoasthenozoospermic men. Int. Braz. J. Urol., 2003, 29, 133-140.
EVENSON D., JOST L. Sperm chromatin structure assay is useful for fertility assessment. Methods Cell Sci., 2000, 22, 169-189.
FARSTAD W. Mating and Artificial Insemination in the dog. In: Simpson G., England G., Harvey M. (Eds), Manual of small animal reproduction and neonatology. British Small Animal Veterinary Association: Cheltenham, 1998, 95-104.
GADELLA B.M. Gamete membrane technology: What is required for mammalian fertilization and how is this challenged during gamete processing? Reprod. Domest. Anim., 2007, 42: Suppl 2, 62-63.
GADELLA B.M. The assembly of a zona pellucida binding protein complex in sperm. Reprod. Domest. Anim., 2008a, 43, 12-19.
GADELLA B.M. Sperm membrane physiology and relevance for fertilization. Anim. Reprod. Sci., 2008b, 107, 229-236.
GADELLAB.M., TSAI P.S., BOERKE A., BREWIS I.A. Sperm head membrane reorganisation during capacitation. Int. J. Dev. Biol., 2008, 52, 473-480.
GILMORE J.A., LIU J., WOODS E.J., PETER A.T., CRITSER J.K. Cryoprotective agent and temperature effects on human sperm membrane permeabilities: convergence of theoretical and empirical approaches for optimal cryopreservation methods. Hum. Reprod., 2000, 15, 335-343.
GIRAUD M.N., MOTTA C., BOUCHER D., GRIZARD G. Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Hum. Reprod., 2000, 15, 2160-2164.
GLANDER H.J., SCHALLER J., LADUSCH M. Eine einfache Methode zur Separierung von frischen und kryokonservierten Humanspermien mittels einer Dextran-Visotrast-Dichtegradientenzentrifugation. Zentralbl. Gynakol., 1990, 112, 91-97.
GRAVANCE C.G., CHAMPION Z., LIU I.K., CASEY P.J. Sperm head morphometry analysis of ejaculate and dismount stallion semen samples. Anim. Reprod. Sci., 1997, 47, 149-155.
HENKEL R. The impact of oxidants on sperm function. Andrologia, 2005, 37, 205-206.
HENKEL R., KIERSPEL E., STALF T., MEHNERT C., MENKVELD R., TINNEBERG H.R., SCHILL W.B., KRUGER T.F. Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in nonleukocytospermic patients. Fertil. Steril., 2005, 83, 635-642.
HOLT W.V. Alternative strategies for the long-term preservation of spermatozoa. Reprod. Fertil. Dev., 1997, 9, 309-319.
HOOGEWIJS M., DE VLIEGHER S., DE SCHAUWER C., GOVAERE J., SMITS K., HOFLACK G., DE KRUIF A., VAN SOOM A. Validation and usefulness of the sperm quality analyzer V equine for equine semen analysis. Theriogenology, 2011, 75, 189-194.
HOOGEWIJS M., RIJSSELAERE T., DE VLIEGHER S., VANHAESEBROUCK E., DE SCHAUWER C., GOVAERE J., THYS M., HOFLACK G., VAN SOOM A., DE KRUIF A. Influence of different centrifugation protocols on equine semen preservation. Theriogenology, 2010, 74, 118-126.
HOSSAIN A.M., OSUAMKPE C.O. Sole use of sucrose in human sperm cryopreservation. Arch. Androl., 2007, 53, 99-103.
HUTCHON S., THORNTON S., HALL J., BISHOP M. Frozen-thawed epididymal sperm is effective for intracytoplasmic sperm injection: implications for the urologist. Br. J. Urol., 1998, 81, 607-611.
IRVINE D.S., TWIGG J.P., GORDON E.L., FULTON N., MILNE P.A., AITKEN R.J. DNA integrity in human spermatozoa: relationships with semen quality. J. Androl., 2000, 21, 33-44.
ISACHENKO E., ISACHENKO V., KATKOV, II, DESSOLE S., NAWROTH F. Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success. Reprod. Biomed. Online, 2003, 6, 191-200.
ISACHENKO E., ISACHENKO V., KATKOV, II, RAHIMI G., SCHONDORF T., MALLMANN P., DESSOLE S., NAWROTH F. DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification. Hum. Reprod., 2004, 19, 932-939.
ISACHENKO V., ISACHENKO E., MONTAG M., ZAEVA V., KRIVOKHARCHENKO I., NAWROTH F., DESSOLE S., KATKOV, II, VAN DER VEN H. Clean technique for cryoprotectant-free vitrification of human spermatozoa. Reprod. Biomed. Online, 2005, 10, 350-354.
JANG H.Y., KIM Y.H., CHEONG H.T., KIM J.T., PARK I.C., PARK C.K., YANG B.K. Curcumin attenuates hydrogen peroxide induced oxidative stress on semen characteristics during in vitro storage of boar semen. Reprod. Dev. Biol., 2009, 33, 99-105.
JOHANSSON C.S., MATSSON F.C., LEHN-JENSEN H., NIELSEN J.M., PETERSEN M.M. Equine spermatozoa viability comparing the NucleoCounter SP-100 and the eosin-nigrosin stain. Anim. Reprod. Sci., 2008, 107, 325-326.
KARESKOSKI A.M., REILAS T., SANKARI S., ANDERSSON M., KATILA T. Composition of fractionated stallion ejaculates. Anim. Reprod. Sci., 2005, 89, 228-230.
KHLIFAOUI M., BATTUT I., BRUYAS J.F., CHATAGNON G., TRIMECHE A., TAINTURIER D. Effects of glutamine on post-thaw motility of stallion spermatozoa: an approach of the mechanism of action at spermatozoa level. Theriogenology, 2005, 63, 138-149.
KNOP K., HOFFMANN N., RATH D., SIEME H. Evaluation of slow cooling after centrifugation and glycerol addition at 22 degrees C versus direct freezing of semen in stallions with good and poor sperm longevity. Anim. Reprod. Sci., 2005, 89, 299-302.
KOBAYASHI T., KANEKO S., HARA I., PARK Y.J., SATO H., OHNO T., NOZAWA S. Concentrating human sperm before cryopreservation. Andrologia, 1991, 23, 25-28.
KÖSS C., JANETT F., BOLLWEIN H. Effects of different extenders on production of reactive oxygen species and lipid peroxidation of cryopreserved bovine sperm. Reprod. Domest. Anim., 2007, 42: Suppl 2, 80.
KUTZLER M.A. Semen collection in the dog. Theriogenology, 2005, 64, 747-754.
LEEB H., RENNHOFER G. Ueber die Beurteilung der Vitalitat menschlicher Spermien mit der Eosin-Nigrosin-Farbung nach Blom. Wien. Med. Wochenschr., 1954, 104, 877-878.
LINDE-FORSBERG C. Achieving canine pregnancy by using frozen or chilled extended semen. Vet. Clin. North Am. Small Anim. Pract., 1991, 21, 467-485.
LOOMIS P.R. Advanced methods for handling and preparation of stallion semen. Vet. Clin. North Am. Small Anim. Pract., 2006, 22, 663-676.
LOPEZ M.L., OLEA N., RETAMAL C.A. Post-testicular changes in the density and distribution of intramembrane particles of stallion sperm surface domains. Anim. Reprod. Sci., 2007, 100, 204-210.
MAGISTRINI M., CHANTELOUBE P., PALMERE. Influenceofseason and frequency of ejaculation on production of stallion semen for freezing. J. Reprod. Fertil. Suppl., 1987, 35, 127-133.
MANIA-PRAMANIK J., POTDAR S.S., VADIGOPPULA A., SAWANT S. Elastase: a predictive marker of inflammation and/or infection. J. Clin. Lab. Anal., 2004, 18, 153-158.
MESEGUER M., GARRIDO N., SIMON C., PELLICER A., REMOHI J. Concentration of glutathione and expression of glutathione peroxidases 1 and 4 in fresh sperm provide a forecast of the outcome of cryopreservation of human spermatozoa. J. Androl., 2004, 25, 773-780.
MICHAEL A., ALEXOPOULOS C., PONTIKI E., HADJIPAVLOU-LITINA D., SARATSIS P., BOSCOS C. Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology, 2007, 68, 204-212.
MILLER D. Spermatozoal RNA as reservoir, marker and carrier of epigenetic information: implications for cloning. Reprod. Domest. Anim., 2007, 42: Suppl 2, 2-9.
MOORE A.I., SQUIRES E.L., GRAHAM J.K. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology, 2005a, 51, 241-249.
MOORE A.I., SQUIRES E.L., GRAHAM J.K. Effect of seminal plasma on the cryopreservation of equine spermatozoa. Theriogenology, 2005b, 63, 2372-2381.
MORRELL J.M. Update on semen technologies for animal breeding. Reprod. Domest. Anim., 2006, 41, 63-67.
NAWROTH F., ISACHENKO V., DESSOLE S., RAHIMI G., FARINA M., VARGIU N., MALLMANN P., DATTENA M., CAPOBIANCO G., PETERS D., ORTH I., ISACHENKO E. Vitrification of human spermatozoa without cryoprotectants. Cryo Letters, 2002, 23, 93-102.
NEILD D. M., BROUWERS J.F., COLENBRANDER B., AGUERO A., GADELLA B.M. Lipid peroxide formation in relation to membrane stability of fresh and frozen thawed stallion spermatozoa. Mol. Reprod. Dev., 2005, 72, 230-238.
NIJS M., OMBELET W. Cryopreservation of human sperm. Hum. Fertil. (Camb.), 2001, 4, 158-163.
NUNEZ-MARTINEZ I., MORAN J.M., PENA F.J. Sperm indexes obtained using computer-assisted morphometry provide a forecast of the freezability of canine sperm. Int. J. Androl., 2007, 30, 182-189.
OLIVEIRA C.H., VASCONCELOS A.B., SOUZA F.A., MARTINSFILHO O.A., SILVA M.X., VARAGO F.C., LAGARES M.A. Cholesterol addition protects membrane intactness during cryopreservation of stallion sperm. Anim. Reprod. Sci., 2010, 118, 194-200.
ORTEGA FERRUSOLA C., GONZALEZ FERNANDEZ L., MORRELL J.M., SALAZAR SANDOVAL C., MACIAS GARCIA B., RODRIGUEZMARTINEZ H., TAPIA J.A., PENA F.J. Lipid peroxidation, assessed with BODIPY-C11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic-like changes. Reproduction, 2009a, 138, 55-63.
ORTEGA FERRUSOLA C., GARCIA B.M., GALLARDO BOLANOS J.M., GONZALEZ FERNANDEZ L., RODRIGUEZ MARTINEZ H., TAPIA J.A., PENA F.J. Apoptotic markers can be used to forecast the freezeability of stallion spermatozoa. Anim. Reprod. Sci., 2009b, 114, 393-403.
PASQUALOTTO F.F., SHARMA R.K., NELSON D.R., THOMAS A.J., AGARWAL A. Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil. Steril., 2000, 73, 459-464.
PAZ G., YOGEV L., GOTTREICH A., ROTEM R., YAVETZ H., HOMONNAI Z.T. The use of an electric freezer in human semen banking. Eur. J. Obstet. Gynecol. Reprod. Biol., 1991, 38, 141-144.
PEGG D.E. The role of vitrification techniques of cryopreservation in reproductive medicine. Hum. Fertil. (Camb.), 2005, 8, 231-239.
PENA A., LINDE-FORSBERG C. Effects of Equex, one-or two-step dilution, and two freezing and thawing rates on post-thaw survival of dog spermatozoa. Theriogenology, 2000, 54, 859-875.
PICKETT B.W. Reproductive evaluation of the stallion. In: McKinnon A.O., Voss J.L. (Eds), Equine reproduction. Williams & Wilkins: Philadelphia, 1993, 755-768.
PILLET E., BATELLIER F., DUCHAMP G., FURSTOSS V., LE VERN Y., KERBOEUF D., DESHERCES S., SCHMITT E., MAGISTRINI M. High fertility rates with stallion sperm cryopreserved in INRA96 based extender are not predicted by in vitro parameters. Anim. Reprod. Sci., 2008, 107, 339-340.
PILLET E., DUCHAMP G., BATELLIER F., BEAUMAL V., ANTON M., DESHERCES S., SCHMITT E., MAGISTRINI M. Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology, 2011, 75, 105-114.
PONTHIER J., DE LA REBIERE DE POUYADE G., DESVALS M., SPALART M., FRANCK T., SERTEYN D., DELEUZE S. Is neutrophil elastase associated with myeloperoxidase concentration and post-thawing parameters in equine frozen semen? Anim. Reprod. Sci., 2010a, 121S, S200-S202.
PONTHIER J., FRANCK T., DETILLEUX J., MOTTART E., SERTEYN D., DELEUZE S. Association between myeloperoxidase concentration in equine frozen semen and post-thawing parameters. Reprod. Domest. Anim., 2010b, 45, 811-816.
PONTHIER J., DESVALS M., FRANCK T., DE LA REBIERE DE POUYADE G., SPALART M., PALMER E., SERTEYN D., DELEUZE S. Myeloperoxidase in equine semen: concentration and localization during freezing processing. J. Equine Vet. Sci., 2012, 32, 32-37.
PONTHIER J., FRANCK T., MOTTART E., SERTEYN D., DELEUZE S. Equine frozen semen parameters in relation with total myeloperoxidase concentration. Anim. Reprod. Sci., 2008, 107, 40-41.
PONTHIER J., TEAGUE S.R., FRANCK T., DE LA REBIERE DE POUYADE G., SERTEYN D., BRINSKO S.P., LOVE C.C., BLANCHARD T.L., VARNER D.D., DELEUZE S. Effect of non-sperm cells removal with density gradient centrifugations on Myeloperoxidase concentration in post-thaw equine semen. Theriogenology, 2013, 80, 1082-1087.
PRATHALINGAM N.S., HOLT W.V., REVELL S.G., MIRCZUK S., FLECK R.A., WATSON P.F. Impact of antifreeze proteins and antifreeze glycoproteins on bovine sperm during freeze-thaw. Theriogenology, 2006, 66, 1894-1900.
ROYERE D., BARTHELEMY C., HAMAMAH S., LANSAC J. Cryopreservation of spermatozoa: a 1996 review. Hum. Reprod. Update, 1996, 2, 553-559.
SAID T.M., AZIZ N., SHARMA R.K., LEWIS-JONES I., THOMAS A.J., JR., AGARWAL A. Novel association between sperm deformity index and oxidative stress-induced DNA damage in infertile male patients. Asian J. Androl., 2005, 7, 121-126.
SALAZAR J.L., HAYDEN S.S., WAITE J.A., COMERFORD K.L., EDMOND A.J., TEAGUE S.R., LOVE C.C., VARNER D.D. Effect of cryopreservation protocol on post-thaw characteristics of stallion spermatozoa. Anim. Reprod. Sci., 2008, 107, 347-348.
SAMPER J.C. Management and fertility of mares bred with frozen semen. Anim. Reprod. Sci., 2001, 68, 219-228.
SCHAFER-SOMI S., KLUGER S., KNAPP E., KLEIN D., AURICH C. Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa. Theriogenology, 2006, 66, 173-182.
SCHEMBRI M.A., MAJOR D.A., SUTTIE J.J., MAXWELL W.M., EVANS G. Capacitation-like changes in equine spermatozoa throughout the cryopreservation process. Reprod. Fertil. Dev., 2002, 14, 225-233.
SCHOBER D., AURICH C., NOHL H., GILLE L. Influence of cryopreservation on mitochondrial functions in equine spermatozoa. Theriogenology, 2007, 68, 745-754.
SENGERP.L. Pathways to pregnancy and parturition. Current Conceptions: Pullman, 2005, 373 p.
SIEME H., KATILA T., KLUG E. Effect of semen collection practices on sperm characteristics before and after storage and on fertility of stallions. Theriogenology, 2004, 61, 769-784.
SION B., JANNY L., BOUCHER D., GRIZARD G. Annexin V binding to plasma membrane predicts the quality of human cryopreserved spermatozoa. Int. J. Androl., 2004, 27, 108-114.
SOSTARIC E., AALBERTS M., GADELLA B.M., STOUT T.A.E. The roles of the epididymis and prostasomes in the attainment of fertilizing capacity by stallion sperm. Anim. Reprod. Sci., 2008a, 107, 237-248.
SOSTARIC E., DIELEMAN S.J., VAN DE LEST C.H.A., COLENBRANDER B., VOS P.L.A.M., GARCIA-GIL N., GADELLA B.M. Sperm binding properties and secretory activity of the bovine oviduct immediately before and after ovulation. Mol. Reprod. Dev., 2008b, 75, 60-74.
SQUIRES E.L. Integration of future biotechnologies into the equine industry. Anim. Reprod. Sci., 2005, 89, 187-198.
SQUIRES E.L., KEITH S.L., GRAHAM J.K. Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology, 2004, 62, 1056-1065.
THIRUMALA S., FERRER M.S., AL-JARRAH A., EILTS B.E., PACCAMONTI D.L., DEVIREDDY R. V. Cryopreservation of canine spermatozoa: theoretical prediction of optimal cooling rates in the presence and absence of cryoprotective agents. Cryobiology, 2003, 47, 109-124.
THOMAS A.D., MEYERS S.A., BALL B.A. Capacitation-like changes in equine spermatozoa following cryopreservation. Theriogenology, 2006, 65, 1531-1550.
TORRES-BOGGINO F., SATO K., OKA A., KANNO Y., HOCHI S., OGURI N., BRAUN J. Relationship among seminal characteristics, fertility and suitability for semen preservation in draft stallions. J. Vet. Med. Sci., 1995, 57, 225-229.
VARELA A.S., CORCINI C.D., ULGUIM R.R., ALVARENGA M.V.F., BIANCHI I., CORREA M.N., LUCIA T., DESCHAMPS J.C. Effect of low density lipoprotein on the quality of cryopreserved dog semen. Anim. Reprod. Sci., 2009, 115, 323-327.
VIDAMENT M. French field results (1985-2005) on factors affecting fertility of frozen stallion semen. Anim. Reprod. Sci., 2005, 89, 115-136.
VIDAMENT M., DUPERE A.M., JULIENNE P., EVAIN A., NOUE P., PALMER E. Equine frozen semen: freezability and fertility field results. Theriogenology, 1997, 48, 907-917.
VIDAMENT M., ECOT P., NOUE P., BOURGEOIS C., MAGISTRINI M., PALMER E. Centrifugation and addition of glycerol at 22 degres C instead of 4 degrees C improve post-thaw motility and fertility of stallion spermatozoa. Theriogenology, 2000, 54, 907-919.
VIDAMENT M., VINCENT P., YVON J.M., BRUNEAU B., MARTIN F.X. Glycerol in semen extender is a limiting factor in the fertility in asine and equine species. Anim. Reprod. Sci., 2005, 89, 302-305.
VIDAMENT M., YVON J.M., COUTY I., ARNAUD G., NGUEKAM-FEUGANG J., NOUE P., COTTRON S., LE TELLIER A., NOEL F., PALMER E., MAGISTRINI M. Advances in cryopreservation of stallion semen in modified INRA82. Anim. Reprod. Sci., 2001, 68, 201-218.
VOLPES A., SAMMARTANO F., COFFARO F., GUIDA S., SCAGLIONE P., ALLEGRA A. Is it possible to use the hypoosmotic swelling test as criteria for "freezeability" of human semen in an AID program? Acta Eur. Fertil., 1992, 23, 191-194.
WAITE J.A., LOVE C.C., BRINSKO S.P., TEAGUE S.R., SALAZAR J.L., JR., MANCILL S.S., VARNER D.D. Factors impacting equine sperm recovery rate and quality following cushioned centrifugation. Theriogenology, 2008, 70, 704-714.
WANG A., FANNING L., ANDERSON D.J., LOUGHLIN K.R. Generation of reactive oxygen species by leukocytes and sperm following exposure to urogenital tract infection. Arch. Androl., 1997a, 39, 11-17.
WANG A.W., ZHANG H., IKEMOTO I., ANDERSON D.J., LOUGHLIN K.R. Reactive oxygen species generation by seminal cells during cryopreservation. Urology, 1997b, 49, 921-925.
WANG Y., SHARMA R.K., AGARWAL A. Effect of cryopreservation and sperm concentrat ion on lipid peroxidation in human semen. Urology, 1997c, 50, 409-413.
WOELDERS H., CHAVEIRO A. Theoretical prediction of 'optimal' freezing programmes. Cryobiology, 2004, 49, 258-271.
ZAVOS P.M., SOFIKITIS N., TODA T., MIYAGAWA I. Improvements in qualitative characteristics of cryopreserved human spermatozoa following recovery via the SpermPrep II filtration method. Tohoku J. Exp. Med., 1991, 165, 283-290.