glycolipid; membrane interaction; cholesterol influence; Langmuir monolayer; isothermal titration calorimetry; IR spectroscopy; in silico analysis
Abstract :
[en] Glycolipids constitute a class of molecules with various biological activities. Among them,
sugar-based bolaamphiphiles characterized by their biocompatibility, biodegradability and
lower toxicity, became interesting for the development of efficient and low cost lipid-based
drug delivery systems. Their activity seems to be closely related to their interactions with the
lipid components of the plasma membrane of target cells. Despite many works devoted to the
chemical synthesis and characterization of sugar-based bolaamphiphiles, their interactions
with plasma membrane have not been completely elucidated. In this work, two sugar-based
bolaamphiphiles differing only at the level of their sugar residues were chemically
synthetized. Their interactions with membranes have been investigated using model
membranes containing or not sterol and with in silico approaches. Our findings indicate that
the nature of sugar residues has no significant influence for their membrane interacting
properties, while the presence of sterol attenuates the interactions of both bolaamphiphiles
with the membrane systems. The understanding of this distinct behavior of bolaamphiphiles
towards sterol-containing membrane systems could be useful for their applications as drug
delivery systems.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Nasir, Mehmet Nail ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie générale et organique
Crowet, Jean-Marc ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biophysique moléc. aux interfaces
Lins, Laurence ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biophysique moléc. aux interfaces
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