Bronchoalveolar lavage fluid cytology and cytokine messenger ribonucleic Acid expression of racehorses with exercise intolerance and lower airway inflammation.
Lavoie, J. P.; Cesarini Latorre, Carlota; Lavoie-Lamoureux, A.et al.
2011 • In Journal of Veterinary Internal Medicine, 25 (2), p. 322-9
[en] BACKGROUND: There is limited information relating bronchoalveolar lavage (BAL) cytology and cytokine messenger ribonucleic acid (mRNA) expression in racehorses with inflammatory airway disease (IAD). HYPOTHESIS AND OBJECTIVE: We hypothesize that cytokine expression in BAL cells would correlate with cytology. Thus, we evaluated the mRNA expression of selected cytokines in BAL cells in racehorses with exercise intolerance and lower airway inflammation. ANIMALS: Thirty-one client-owned Standardbred racehorses with exercise intolerance. METHODS: Prospective, observational study. Cells were obtained by BAL, and mRNA expression of interleukin (IL)-1beta, IL-4, IL-8, tumor necrosis factor (TNF)-alpha, and interferon (IFN)-gamma was determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR). RESULTS: Nine horses had normal BAL cell differential cytology (Controls), while 22 horses had evidence of IAD based on BAL fluid cytology. Relative expressions of TNF-alpha/glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 0.0092 +/- 0.010 versus 0.0045 +/- 0.005, P= .034), IL-4/GAPDH (0.001 +/- 0.002 versus 0.0003 +/- 0.0003, P= .029), and IFN-gamma/GAPDH (0.0027 +/- 0.003 versus 0.0009 +/- 0.001, P= .028) were greater in horses with IAD compared with controls. Furthermore, IL-4/GAPDH (0.001 +/- 0.002 versus 0.0002 +/- 0.0003, P < .0001) and IFN-gamma/GAPDH (0.003 +/- 0.003 versus 0.001 +/- 0.001, P= .002) mRNA expression was increased in horses with increased metachromatic cell counts compared with horses with normal metachromatic cell counts. Only the mRNA expression of IL-1beta/GAPDH (1.1 +/- 0.7 versus 0.3 +/- 0.3, P= .045) was increased with airway neutrophilia. CONCLUSIONS AND CLINICAL IMPORTANCE: Differences in gene expression were associated with the presence of IAD and with specific cell types present in airway secretions of Standardbred racehorses with poor performance. These findings suggest that different pathophysiological pathways are implicated in IAD.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Lavoie, J. P.
Cesarini Latorre, Carlota ; Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés
Lavoie-Lamoureux, A.
Moran, K.
Lutz, S.
Picandet, V.
Jean, D.
Marcoux, M.
Language :
English
Title :
Bronchoalveolar lavage fluid cytology and cytokine messenger ribonucleic Acid expression of racehorses with exercise intolerance and lower airway inflammation.
Publication date :
2011
Journal title :
Journal of Veterinary Internal Medicine
ISSN :
0891-6640
eISSN :
1939-1676
Publisher :
Wiley, Malden, United States - Massachusetts
Volume :
25
Issue :
2
Pages :
322-9
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2011 by the American College of Veterinary Internal Medicine.
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