Conceptus; Hormone; Placenta well-being; Neosporosis; Embryo transfer
Abstract :
[en] Neospora caninum is an intracellular coccidian parasite causing abortion worldwide in dairy cattle. Studies have shown that N caninum infection modifies endocrine patterns and that beef cows or cows with a crossbreed pregnancy, especially for a greater maternal-paternal genetic distance, show a reduced risk of abortion when naturally infected compared with pure-breed Holstein Friesian (HF) pregnancies. This study examined the effects of crossbreeding on plasma progesterone (P4), pregnancy-associated glycoprotein (PAG)-1 and -2, and prolactin patterns produced during gestation in N caninum-infected beef/dairy cows. We analyzed 74 pregnancies in nonaborting cows carrying a single fetus established in 26 lactating HF cows bred with Holstein bulls (H-H), 13 HF cows bred with Limousin bulls (H-L), 12 HF cows used as recipients of transferred in vivo-produced frozen-thawed Rubia Gallega (beef breed; RG) embryos (H-RG-ET), and 23 RG suckling cows bred with RG bulls (RG-RG). Of the cows, 29 (39%) were seropositive for N caninum. Blood samples for hormone and placental protein determinations were collected on Days 30, 60, 90, 120, 150, and 180 of gestation. Procedures of repeated measures analysis of variance throughout gestation revealed higher PAG-1 levels in the H-L and H-RG-ET groups compared with H-H and RG-RG. Higher prolactin levels were detected in RG-RG than in the remaining groups. N caninum seropositivity and fetal genotype had a significant effect on PAG-2 concentrations, such that highest levels of PAG-2 occurred in RG-RG seropositive cows and lowest in H-H seropositive cows, whereas Neospora-seropositive cows showed lower P4 concentrations than their seronegative partners. In conclusion, chronic N caninum infection modifies endocrine patterns of PAG-2 and P4 during pregnancy, whereas PAG-1 and prolactin concentrations are affected by breed and fetal genotype, irrespective of Neospora infection status.
Research Center/Unit :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Garcia-Ispierto, I.; University of Lleida, Spain > Agrotecnio Center
Serrano-Perez, B.; Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain > Departament de Sanitat i d’Anatomia Animals, Facultat de Veterinària and Centre de Recerca en Sanitat Animal (CReSA)
Almeria, S.; Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain > Departament de Sanitat i d’Anatomia Animals, Facultat de Veterinària and Centre de Recerca en Sanitat Animal (CReSA)
Martinez-Bello, D.; University of Lleida, Spain > Agrotecnio Center
Tchimbou, A. F.; Université de Liège - ULiège > Physiology of Reproduction, Faculty of Veterinary Medicine
Melo de Sousa, Noelita ; Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Beckers, Jean-François ; Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Lopez-Gatius, F.; University of Lleida, Spain > Agrotecnio Center
Language :
English
Title :
Effects of crossbreeding on endocrine patterns determined in pregnant beef/dairy cows naturally infected with Neospora caninum.
Publication date :
2015
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier, Netherlands
Volume :
83
Issue :
4
Pages :
491-496
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
This work was supported by the European FEDER and Spanish CICYT, grants AGL2012-39830-C02-01/GAN and AGL2012-39830-C02-02/GAN
Hunter R.H., Einer-Jensen N. Potential amplification of early pregnancy signals by ovarian follicular cells in suspension within the fallopian tube. Zygote 2003, 11:237-243.
Wolf E., Arnold G.J., Bauersachs S., Beier H.M., Blum H., Einspanier R., et al. Embryo-maternal communication in bovine - strategies for deciphering a complex cross-talk. Reprod Domest Anim 2003, 38:276-289.
Gootwine E. Placental hormones and fetal-placental development. Anim Reprod Sci 2004, 82-83:551-566.
Veenstra van Nieuwenhoven A.L., Heineman M.J., Faas M.M. The immunology of successful pregnancy. Hum Reprod Update 2003, 9:347-357.
Druckmann R., Druckmann M.A. Progesterone and the immunology of pregnancy. J Steroid Biochem Mol Biol 2005, 97:389-396.
Aagaard-Tillery K.M., Silver R., Dalton J. Immunology of normal pregnancy. Semin Fetal Neonatal Med 2006, 11:279-295.
Ayad A., Sousa N.M., Sulon J., Hornick J.L., Watts J., Lopez-Gatius F., et al. Influence of progesterone concentrations on secretory functions of trophoblast and pituitary during the first trimester of pregnancy in dairy cattle. Theriogenology 2007, 67:1503-1511.
Garbayo J.M., Serrano B., López-Gatius F. Identification of novel pregnancy-associated glycoproteins (PAG) expressed by the peri-implantation conceptus of domestic ruminants. Anim Reprod Sci 2008, 103:120-134.
García-Ispierto I., López-Gatius F., Almería S., Yániz J., Santolaria P., Serrano B., et al. Factors affecting plasma prolactin concentrations throughout gestation in high producing dairy cows. Domest Anim Endocrinol 2009, 36:57-66.
García-Ispierto I., Almería S., Serrano B., de Sousa N.M., Beckers J.F., López-Gatius F. Plasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine PAG-2 antibodies on day 120 of gestation predict abortion in dairy cows naturally infected with Neospora caninum. Reprod Domest Anim 2013, 48:613-618.
Abdelfatah-Hassan A., Almería S., Serrano B., de Sousa N.M., Beckers J.F., López-Gatius F. The inseminating bull and plasma pregnancy-associated glycoprotein (PAG) levels were related to peripheral leukocyte counts during the late pregnancy/early postpartum period in high-producing dairy cows. Theriogenology 2012, 77:1390-1397.
García-Ispierto I., Nogareda C., Yániz J.L., Almería S., Martínez-Bello D., de Sousa N.M., et al. Neospora caninum and Coxiella burnetii seropositivity are related to endocrine pattern changes during gestation in lactating dairy cows. Theriogenology 2010, 74:212-220.
Rosbottom A., Gibney H., Kaiser P., Hartley C., Smith R.F., Robinson R., et al. Up regulation of the maternal immune response in the placenta of cattle naturally infected with Neospora caninum. PLoS One 2011, 6:e15799.
Almería S., Serrano B., Yàniz J.L., Darwich L., López-Gatius F. Cytokine gene expression profiles in peripheral blood mononuclear cells from Neospora caninum naturally infected dams throughout gestation. Vet Parasitol 2012, 183:237-243.
Dubey J.P., Schares G. Neosporosis in animals-the last five years. Vet Parasitol 2011, 180:90-108.
Almería S., López-Gatius F. Bovine neosporosis: clinical and practical aspects. Res Vet Sci 2013, 95:303-309.
De Meerschman F., Speybroeck N., Berkvens D., Rettigner C., Focant C., Leclipteux T., et al. Fetal infection with Neospora caninum in dairy and beef cattle in Belgium. Theriogenology 2002, 58:933-945.
López-Gatius F., Santolaria P., Yániz J.L., Garbayo J.M., Almería S. The use of beef bull semen reduces the risk of abortion in Neospora-seropositive dairy cows. J Vet Med B Infect Dis Vet Public Health 2005, 52:88-92.
Bartels C.J., Arnaiz-Seco J.I., Ruiz-Santa-Quitera A., Björkman C., Frössling J., von Blumröder D., et al. Supranational comparison of Neospora caninum seroprevalences in cattle in Germany, The Netherlands, Spain and Sweden. Vet Parasitol 2006, 137:17-27.
Armengol R., Pabón M., Santolaria P., Cabezón O., Adelantado C., Yániz J., et al. Low seroprevalence of Neospora caninum infection associated with the Limousin breed in cow-calf herds in Andorra, Europe. J Parasitol 2007, 93:1029-1032.
Almería S., López-Gatius F., García-Ispierto I., Nogareda C., Bech-Sàbat G., Serrano B., et al. Effects of crossbreed pregnancies on the abortion risk of Neospora caninum-infected dairy cows. Vet Parasitol 2009, 163:323-329.
Serrano B., López-Gatius F., Santolaria P., Almería S., García-Ispierto I., Bech-Sabat G., et al. Factors affecting plasma pregnancy-associated glycoprotein 1 concentrations throughout gestation in high-producing dairy cows. Reprod Domest Anim 2009, 44:600-605.
Serrano-Pérez B., Garcia-Ispierto I., de Sousa N., Beckers J., Almería S., López-Gatius F. Gamma interferon production and plasma concentrations of pregnancy-associated glycoproteins 1 and 2 in gestating dairy cows naturally infected with Neospora caninum. Reprod Domest Anim 2014, 49:275-280.
Santolaria P., Almería S., Martínez-Bello D., Nogareda C., Mezo M., Gonzalez-Warleta M., et al. Different humoral mechanisms against Neospora caninum infection in purebreed and crossbreed beef/dairy cattle pregnancies. Vet Parasitol 2011, 178:70-76.
Nutrient requirements of dairy cattle 2001, National Research Council, National Academic Science, Washington, DC. Seventh review edition.
Bungartz L., Niemann H. Assessment of the presence of a dominant follicle and selection of dairy cows suitable for superovulation by a single ultrasound examination. J Reprod Fertil 1994, 101:583-591.
Bergfelt D.R., Bo G.A., Mapletoft R.J., Adams G.P. Superovulatory response following ablation-induced follicular wave emergence at random stages of the oestrous cycle in cattle. Anim Reprod Sci 1997, 49:1-12.
Greenwood F.C., Hunter W.M., Glover J.S. The preparation of 131-I labelled human growth hormone of high specific radioactivity. Biochem J 1963, 89:114-123.
López-Gatius F., Garbayo J.M., Santolaria P., Yániz J., Ayad A., de Sousa N.M., et al. Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses. Domest Anim Endocrinol 2007, 32:29-42.
Vaitukaitis J., Robbins J.B., Nieschlag E., Ross G.T. A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab 1971, 33:988-991.
Bella A, Sousa NM, Lopez-Gatius F, Garcia-Ispierto I, Schoofs L, Chahiba N, Beckers JF: Development of a new radioimmunoassay by using antiserum against pregnancy-associated glycoprotein ii: concentrations in Holstein dairy cattle. 6th European Congress of Bovine Health and Management (ECBHM), 7-9 September, 2011, p63; Liège. (Abstract of Conference).
Beckers J.F., Dewulf M., Verstegen J., Wouters-Ballman P., Ectorsm F. Isolation of a bovine chorionic gonadotrophin (bCG). Theriogenology 1988, 29:218. Abstract.
Thorell J.I., Johansson B.G. Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim Biophys Acta 1971, 251:363-369.
López-Gatius F., López-Béjar M., Murugavel K., Pabón M., Ferrer D., Almería S. Neospora-associated abortion episode over a 1-year period in a dairy herd in north-east Spain. J Vet Med B Infect Dis Vet Public Health 2004, 51:348-352.
López-Gatius F., Pabon M., Almería S. Neospora caninum infection does not affect early pregnancy in dairy cattle. Theriogenology 2004, 62:606-613.
Bech-Sàbat G., López-Gatius F., Santolaria P., García-Ispierto I., Pabón M., Nogareda C., et al. Progesterone supplementation during mid-gestation increases the risk of abortion in Neospora-infected dairy cows with high antibody titres. Vet Parasitol 2007, 145:164-167.
Zoli A.P., Guilbault L.A., Delahaut P., Benitez-Ortiz W., Beckers J.F. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 1992, 46:83-92.
Fernandez-Arias A., Alabart J.L., Folch J., Beckers J.F. Interspecies pregnancy of Spanish ibex (Capra pyrenaica) fetus in domestic goat (Capra hircus) recipients induces abnormally high plasmatic levels of pregnancy-associated glycoprotein. Theriogenology 1999, 51:1419-1430.
Klisch K., Jeanrond E., Pang P.C., Pich A., Schuler G., Dantzer V., et al. A tetraantennary glycan with bisecting N-acetylglucosamine and the Sda antigen is the predominant N-glycan on bovine pregnancy-associated glycoproteins. Glycobiology 2008, 18:42-52.
López-Gatius F., Almería S., Donofrio G., Nogareda C., García-Ispierto I., Bech-Sàbat G., et al. Protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with Neospora caninum. Theriogenology 2007, 68:1067-1073.
Guilbault L.A., Roy G.L., Beckers J.F., Dufour J.J. Influence of breed of fetus on periparturient endocrine responses and subsequent milk production of Ayrshire dams. J Dairy Sci 1990, 73:2766-2773.
Lammoglia M.A., Holloway J.W., Lewis A.W., Neuendorff D.A., Randel R.D. Influence of maternal and service-sire breed on serum progesterone and estrogen before calving and plasma 13,14-dihydro-15-keto-prostaglandin F2 alpha after calving. J Anim Sci 1995, 73:1167-1173.
Svennersten-Sjaunja K., Olsson K. Endocrinology of milk production. Domest Anim Endocrinol 2005, 29:241-258.
Dogusan Z., Hooghe R., Verdood P., Hooghe-Peters E.L. Cytokine-like effects of prolactin in human mononuclear and polymorphonuclear leukocytes. J Neuroimmunol 2001, 120:58-66.
Negrão J.A. Hormone release and behavior during suckling and milking in Gir, Gir × Holstein, and Holstein cows. J Anim Sci 2008, 86:21-26.
Brand J.M., Frohn C., Cziupka K., Brockmann C., Kirchner H., Luhm J. Prolactin triggers pro-inflammatory immune responses in peripheral immune cells. Eur Cytokine Netw 2004, 15:99-104.
Burke J.M., Coleman S.W., Chase C.C., Riley D.G., Looper M.L., Brown M.A. Interaction of breed type and endophyte-infected tall fescue on milk production and quality in beef cattle. J Anim Sci 2010, 88:2802-2811.
