Article (Scientific journals)
Accuracy of diagnosing double corpora lutea and twin pregnancy by measuring serum progesterone and bovine pregnancy-associated glycoprotein 1 in the first trimester of gestation in dairy cows
Szelenyi, Zoltan; Répási, Attila; Melo de Sousa, Noelita et al.
2015In Theriogenology, 84 (1), p. 76-81
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Keywords :
Cattle; Twin; Progesterone; Pregnancy-associated glycoprotein; RIA
Abstract :
[en] Progesterone (P4) and bovine pregnancy-associated glycoprotein 1 (bPAG-1) concentrations during gestation are dependent on the number of CL and fetuses, respectively. The objective of this present study was to measure and evaluate the usefulness of measuring the P4 and bPAG-1 concentrations in cases of single versus twin pregnancies and one versus two CL at the first 4 months of gestation. We hypothesized that both the number of the CL and the number of fetus might have an effect on P4 and pregnancy protein concentrations, and we can set up clinically useful threshold levels to predict twin gestations. Eighty-four Holstein-Friesian dairy cows were enrolled in this prospective observational clinical trial. Blood was collected at time point 1: between Days 29 and 42, time point 2: between Days 57 and 70, time point 3: between Days 85 and 98, and time point 4: between Days 113 and 126 of gestation, and bPAG-1 and P4 concentrations were measured. Binary logistic regression analyzing serum P4 concentrations differed at time point 2 compared with baseline level, but the area under the curve (AUC) had low sensitivity. The bPAG-1 concentrations were statistically different at each time point of gestation. The AUC cutoff values of serum bPAG-1 concentrations were sufficiently sensitive to differentiate between twin gestations from singleton ones. At time points 3 (cutoff value of 3.4 ng/mL) and 4 (cutoff value of 56.5 ng/mL), statistically significant differences with low sensitivity, high specificity, and a high AUC were found. On the basis of these results, the diagnosis of twin pregnancy using pregnancy protein measurements is clinically insufficient before Day 85 of gestation; however, the ability to confirm the early twin pregnancy diagnosis with bPAG-1 measurements appears to be promising. To achieve high sensitivity, further studies are required.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Szelenyi, Zoltan;  Szent Istvan University, Ullo-Dora major, Hungary > Department and Clinic for Production Animals, Faculty of Veterinary Science
Répási, Attila;  MTA-SZIE Large Animal Clinical Research Group, Ullo-Dora major, Hungary
Melo de Sousa, Noelita ;  Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Beckers, Jean-François  ;  Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Szenci, Otto;  Szent Istvan University, Ullo-Dora major, Hungary > Department and Clinic for Production Animals, Faculty of Veterinary Science
Language :
English
Title :
Accuracy of diagnosing double corpora lutea and twin pregnancy by measuring serum progesterone and bovine pregnancy-associated glycoprotein 1 in the first trimester of gestation in dairy cows
Publication date :
2015
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier, New York, United States - New York
Volume :
84
Issue :
1
Pages :
76-81
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 March 2016

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