Accuracy of diagnosing double corpora lutea and twin pregnancy by measuring serum progesterone and bovine pregnancy-associated glycoprotein 1 in the first trimester of gestation in dairy cows
Szelenyi, Zoltan; Répási, Attila; Melo de Sousa, Noelitaet al.
[en] Progesterone (P4) and bovine pregnancy-associated glycoprotein 1 (bPAG-1) concentrations during gestation are dependent on the number of CL and fetuses, respectively. The objective of this present study was to measure and evaluate the usefulness of measuring the P4 and bPAG-1 concentrations in cases of single versus twin pregnancies and one versus two CL at the first 4 months of gestation. We hypothesized that both the number of the CL and the number of fetus might have an effect on P4 and pregnancy protein concentrations, and we can set up clinically useful threshold levels to predict twin gestations. Eighty-four Holstein-Friesian dairy cows were enrolled in this prospective observational clinical trial. Blood was collected at time point 1: between Days 29 and 42, time point 2: between Days 57 and 70, time point 3: between Days 85 and 98, and time point 4: between Days 113 and 126 of gestation, and bPAG-1 and P4 concentrations were measured. Binary logistic regression analyzing serum P4 concentrations differed at time point 2 compared with baseline level, but the area under the curve (AUC) had low sensitivity. The bPAG-1 concentrations were statistically different at each time point of gestation. The AUC cutoff values of serum bPAG-1 concentrations were sufficiently sensitive to differentiate between twin gestations from singleton ones. At time points 3 (cutoff value of 3.4 ng/mL) and 4 (cutoff value of 56.5 ng/mL), statistically significant differences with low sensitivity, high specificity, and a high AUC were found. On the basis of these results, the diagnosis of twin pregnancy using pregnancy protein measurements is clinically insufficient before Day 85 of gestation; however, the ability to confirm the early twin pregnancy diagnosis with bPAG-1 measurements appears to be promising. To achieve high sensitivity, further studies are required.
Research Center/Unit :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Szelenyi, Zoltan; Szent Istvan University, Ullo-Dora major, Hungary > Department and Clinic for Production Animals, Faculty of Veterinary Science
Répási, Attila; MTA-SZIE Large Animal Clinical Research Group, Ullo-Dora major, Hungary
Melo de Sousa, Noelita ; Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Beckers, Jean-François ; Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Szenci, Otto; Szent Istvan University, Ullo-Dora major, Hungary > Department and Clinic for Production Animals, Faculty of Veterinary Science
Language :
English
Title :
Accuracy of diagnosing double corpora lutea and twin pregnancy by measuring serum progesterone and bovine pregnancy-associated glycoprotein 1 in the first trimester of gestation in dairy cows
Youngquist R.S. Pregnancy diagnosis. Current therapy in large animal theriogenology 1997, 295-303. WB Saunders Co., Philadelphia. R.S. Youngquist (Ed.).
Romano J.E., Thompson J.A., Kraemer D.C., Westhusin M.E., Tomaszweski M.A., Forrest D.W. Effects of early pregnancy diagnosis by palpation per rectum on pregnancy loss in dairy cattle. J Am Vet Med Assoc 2011, 239:668-673.
Romano J.E., Thompson J.A., Kraemer D.C., Westhusin M.E., Forrest D.W., Tomaszweski M.A. Early pregnancy diagnosis by palpation per rectum: influence on embryo/fetal viability in dairy cattle. Theriogenology 2007, 67:486-493.
Bech-Sàbat G., López-Gatius F., Yániz J.L., García-Ispierto I., Santolaria P., Serrano B., et al. Factors affecting plasma progesterone in the early fetal period in high producing dairy cows. Theriogenology 2008, 69:426-432.
Semambo D.K.N., Eckersall P.D., Sasser R.G., Ayliffe T.R. Pregnancy-specific protein B and progesterone in monitoring viability of the embryo in early pregnancy in the cow after experimental infection with Actinomyces pyogenes. Theriogenology 1992, 37:741-748.
Szenci O., Beckers J.F., Sulon J., Bevers M.M., Borzsonyi L., Fodor L., et al. Effect of induction of late embryonic mortality on plasma profiles of pregnancy associated glycoprotein 1 in heifers. Vet J 2003, 165:307-313.
Chavatte-Palmer P., de Sousa N., Laigre P., Camous S., Ponter A.A., Beckers J.F., et al. Ultrasound fetal measurements and pregnancy associated glycoprotein secretion in early pregnancy in cattle recipients carrying somatic clones. Theriogenology 2006, 66:829-840.
Friedrich M., Holtz W. Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection. Reprod Domest Anim 2010, 45:142-146.
Dobson H., Rowan T.G., Kippax I.S., Humblot P. Assessment of fetal number, and fetal and placental viability throughout pregnancy in cattle. Theriogenology 1993, 40:411-425.
Patel O.V., Sulon J., Beckers J.F., Takahashi T., Hirako M., Sasaki N., et al. Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow. Eur J Endocrinol 1997, 137:423-428.
López-Gatius F., Garbayo J.M., Santolaria P., Yániz J., Ayad A., de Sousa N.M., et al. Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses. Domest Anim Endocrinol 2007, 32:29-42.
Serrano B., López-Gatius F., Santolaria P., Almería S., García-Ispierto I., Bech-Sabat G., et al. Factors affecting plasma pregnancy-associated glycoprotein 1 concentrations throughout gestation in high-producing dairy cows. Reprod Domest Anim 2009, 44:600-605.
Nutrient requirements of dairycattle 2001, National Research Council, The National Academies Press, Washington, DC. Seventh revised edition.
Szenci O., Gyulay Gy, Nagy P., Kovács L., Varga J., Taverne M.A. Effect of uterus position relative to the pelvic inlet on the accuracy of early bovine pregnancy diagnosis by means of ultrasonography. Vet Q 1995, 17:37-39.
Dieleman S.J., Bevers M.M. Effects of monoclonal antibody against PMSG administered shortly after the preovulatory LH surge on time and number of ovulations in PMSG/PG-treated cows. J Reprod Fertil 1987, 81:533-542.
Zoli A.P., Guilbault L.A., Delahaut P., Ortiz W.B., Beckers J.F. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 1992, 46:83-92.
Perényi Z.S., Szenci O., Sulon J., Drion P.V., Beckers J.F. Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma. Reprod Domest Anim 2002, 37:100-104.
Zoli A.P., Beckers J.F., Wouters-Ballman P., Closset J., Falmagne P., Ectors F. Purification and characterization of a bovine pregnancy-associated glycoprotein. Biol Reprod 1991, 45:1-10.
Del Río N.S., Kirkpatrick B.W., Fricke P.M. Observed frequency of monozygotic twinning in Holstein dairy cattle. Theriogenology 2006, 66:1292-1299.
Wiltbank M.C., Souza A.H., Carvalho P.D., Cunha A.P., Giordano J.O., Fricke P.M., et al. Physiological and practical effects of progesterone on reproduction in dairy cattle. Animal 2014, (Suppl 1):70-81.
Forde N., Beltman M.E., Duffy G.B., Duffy P., Mehta J.P., O'Gaora P., et al. Changes in the endometrial transcriptome during the bovine estrous cycle: effect of low circulating progesterone and consequences for conceptus elongation. Biol Reprod 2011, 84:266-278.
Mann G.E., Robinson R.S., Hunter M.G. Corpus luteum size and function following single and double ovulations in non-lactating dairy cows. Theriogenology 2007, 67:1256-1261.
Mann G.E. Corpus luteum size and plasma progesterone concentration in cows. Anim Reprod Sci 2009, 115:296-299.
Andreu-Vázquez C., Garcia-Ispierto I., López-Gatius F.J. Manual rupture versus transvaginal ultrasound-guided aspiration of allanto-amniotic fluid in multiple pregnancies: a clinical approach to embryo reduction in dairy cattle. J Reprod Dev 2012, 58:420-424.
