Article (Scientific journals)
Neutralization of Bovine Herpesvirus Type 4 by Pairs of Monoclonal Antibodies Raised against Two Glycoproteins and Identification of Antigenic Determinants Involved in Neutralization
Dubuisson, J.; Guillaume, José; Boulanger, D. et al.
1990In Journal of General Virology, 71 ((Pt 3)), p. 647-53
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Abstract :
[en] In infected cattle, bovine herpesvirus type 4 (BHV-4) induces an immune response with low neutralizing antibody levels or in the absence of such antibodies. For the study of this phenomenon, monoclonal antibodies (MAbs) raised against two BHV-4 glycoproteins identified previously (150K/120K/51K and 120K/16.5K) were used in neutralization tests. None of the MAbs except for MAb 16 could neutralize alone; pairs of MAbs against the 150K/120K/51K and 120K/16.5K glycoproteins were able to neutralize BHV-4 infectivity. MAbs involved in neutralization were used in competitive binding assays to identify epitopes relevant for BHV-4 neutralization. These MAbs showed a low avidity and a weak neutralizing activity, and they partially decreased BHV-4 attachment to cells. These results suggest that the BHV-4 glycoprotein domains involved in viral infectivity are poorly exposed to the immune system.
Disciplines :
Veterinary medicine & animal health
Microbiology
Author, co-author :
Dubuisson, J.
Guillaume, José
Boulanger, D.
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Bublot, M.
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Language :
English
Title :
Neutralization of Bovine Herpesvirus Type 4 by Pairs of Monoclonal Antibodies Raised against Two Glycoproteins and Identification of Antigenic Determinants Involved in Neutralization
Publication date :
March 1990
Journal title :
Journal of General Virology
ISSN :
0022-1317
eISSN :
1465-2099
Publisher :
Society for General Microbiology, United Kingdom
Volume :
71
Issue :
(Pt 3)
Pages :
647-53
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 August 2009

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