Article (Scientific journals)
Effects of Human and Salmon Calcitonin on Human Articular Chondrocytes Cultivated in Clusters
Franchimont, Paul; Bassleer, Corinne; Henrotin, Yves et al.
1989In Journal of Clinical Endocrinology and Metabolism, 69 (2), p. 259-66
Peer Reviewed verified by ORBi
 

Files


Full Text
franchimont calci.pdf
Publisher postprint (1.17 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The effects of different pharmacological concentrations (0, 5, 10, 100, and 1000 ng/mL) of synthetic human calcitonin (hCT) and salmon calcitonin (sCT) on the incorporation of [3H]thymidine and production of proteoglycans (PG) and type II collagen (coll II) by human articular chondrocytes during a 20-day period were studied in a tridimensional chondrocyte culture model. [3H]Thymidine uptake was measured in chondrocyte clusters, and specific PG and coll II RIAs were performed every 4 days on the culture medium and cell aggregates; total PG and coll II production were also assessed at different culture durations by adding the amounts found in culture media and their corresponding clusters. Incubation with hCT or sCT did not affect [3H]thymidine uptake regardless of the dose. For each culture period, PG and coll II release into culture medium, cluster content, and total production increased significantly in a dose-dependent manner. Cumulative curves for these parameters showed a progressive significant increase with culture duration at hCT and sCT doses of 0, 5, and 10 ng/mL. Cumulative curves obtained with 10, 100, and 1000 ng/mL were seldom significantly different from one another. No differences emerged between the use of hCT or sCT. Thus, CT exerted no proliferative effect on human articular chondrocytes in tridimensional culture, but displayed a dose-dependent and prolonged stimulatory effect on PG and coll II production. CT may possess chondroprotective properties in addition to its other known effects.
Disciplines :
Rheumatology
Author, co-author :
Franchimont, Paul ;  Université de Liège - ULiège
Bassleer, Corinne ;  Université de Liège - ULiège
Henrotin, Yves  ;  Université de Liège - ULiège > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Gysen, Philippe ;  Université de Liège - ULiège
Bassleer, Roger ;  Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Effects of Human and Salmon Calcitonin on Human Articular Chondrocytes Cultivated in Clusters
Publication date :
August 1989
Journal title :
Journal of Clinical Endocrinology and Metabolism
ISSN :
0021-972X
eISSN :
1945-7197
Publisher :
Oxford University Press, New York, United States - New York
Volume :
69
Issue :
2
Pages :
259-66
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 January 2016

Statistics


Number of views
36 (3 by ULiège)
Number of downloads
3 (3 by ULiège)

Scopus citations®
 
35
Scopus citations®
without self-citations
30
OpenCitations
 
32

Bibliography


Similar publications



Contact ORBi