[en] Context: The in-house techniques or experimental methods are increasingly recommended for
their low-cost reagents for the determination of the Viral Load (VL) in resource-limited settings.
The objective of this study was to compare the determination of VL from HIV-1 non-B samples by
an in-house technique with the COBAS AmpliPrep/TaqMan version 2.0. Method: In this cross-sectional study, 39 plasma samples from patients infected with HIV type 1 non-B from N’Djamena and Kinshasa were used to determine the VL using the two techniques. Results: The mean values of VL are respectively 4.68 ± 1.26 and 4.58 ± 1.33 log10 RNA copies/ml for the COBAS AmpliPrep/TaqMan assays and the in-house assays. A good correlation (Spearman Correlation) was obtained, with a coefficient (R2) of 0.9452. Conclusion: This study demonstrates that there is no significant difference between the results of VL determined by the COBAS AmpliPrep/TaqMan assays and the in-house assays used.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
KAMANGU, Erick
CHATTE, Adawaye
BOREUX, Raphaël ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
SUSIN, Fabrice ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
KALALA, Richard
Mwumbi, Georges Lelo
De Mol, Patrick ; Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Hayette, Marie-Pierre ; Université de Liège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
VAIRA, Dolorès ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
Language :
English
Title :
Comparison of an In-House Quantitative Real-Time PCR and COBAS ampliprep/Taqman Roche for determination of viral load for HIV type 1 non-b
Alternative titles :
[fr] Comparaison d'une RT-PCR in house et du kit COBAS Ampliprep/Taqman Roche pour la détermination de la charge virale HIV
