Reference : Vaccinia virus strain Western Reserve protein B14 is an intracellular virulence factor.
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Vaccinia virus strain Western Reserve protein B14 is an intracellular virulence factor.
Chen, Ron A-J mailto [> > > >]
Jacobs, Nathalie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Smith, Geoffrey L mailto [> > > >]
Journal of General Virology (The)
Society for General Microbiology
Pt 6
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Cell Line ; Escherichia coli/genetics/metabolism ; Female ; Humans ; Mice ; Mice, Inbred C57BL ; Mutation ; Rabbits ; Recombinant Proteins/immunology/isolation & purification ; Subcellular Fractions/metabolism ; Vaccinia/pathology/virology ; Vaccinia virus/genetics/pathogenicity ; Viral Proteins/genetics/metabolism ; Virulence Factors/genetics/metabolism
[en] A characterization of the B14R gene from Vaccinia virus (VACV) strain Western Reserve (WR) is presented. Computational analyses of the B14R gene indicated high conservation in orthopoxviruses but no orthologues outside the Poxviridae. To characterize the B14 protein, the B14R gene was expressed in Escherichia coli and recombinant protein was purified and used to generate a rabbit polyclonal antiserum. This antiserum recognized a 15 kDa cytoplasmic protein in mammalian cells that were transfected with the B14R gene or infected with VACV WR, but not from cells infected with a VACV mutant (vdeltaB14) from which the B14R gene was deleted. Compared to wild-type and revertant virus controls, vdeltaB14 had normal growth kinetics in cell culture. The virulence of vdeltaB14 was assessed in two in vivo models. Mice infected intranasally with vdeltaB14 had similar weight loss compared to the controls, but in C57BL/6 mice infected intradermally vdeltaB14 induced a smaller lesion size compared with controls. Moreover, intradermal infection with vdeltaB14 caused an increased infiltration of cells into the infected lesion despite the smaller lesion size. Therefore, B14 is an intracellular protein that is non-essential for virus replication in cell culture but contributes to virus virulence in vivo and affects the host response to infection.
Wellcom Trust
Researchers ; Students

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