Influence of the crosslinker type on the chromatographic properties of hydrophilic sulfoalkylbetaine-type monolithic columns.

Liu, Chusheng; Chen, Weijia; Yuan, Guangxin; Xiao, Yao; Crommen, Jacques; Xu, Shihai; Jiang, Zhengjin

doi:10.1016/j.chroma.2014.11.007

Article (Scientific journals)

Influence of the crosslinker type on the chromatographic properties of hydrophilic sulfoalkylbetaine-type monolithic columns.

Liu, Chusheng; Chen, Weijia; Yuan, Guangxin et al.

2014 • In Journal of Chromatography. A, 1373, p. 73-80

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/187175

DOI
10.1016/j.chroma.2014.11.007

PubMed
25464999

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Keywords :

Acrylamides/chemistry; Betaine/chemistry; Chromatography, High Pressure Liquid/instrumentation/methods; Cross-Linking Reagents/chemistry; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Methacrylates/chemistry; Nucleosides/analysis; Permeability; Polymerization; Porosity; Static Electricity; Crosslinker; Hydrophilic interaction chromatography; N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine; Sulfoalkylbetaine; Zwitterionic monolithic column

Abstract :

[en] In order to investigate the effects of the crosslinker on the separation performance of polar zwitterionic sulfoalkylbetaine-type monolithic columns, three crosslinkers, i.e. 1,4-bis(acryloyl)piperazine (PDA), ethylene dimethacrylate (EDMA) and N,N'-methylenebisacrylamide (MBA), were copolymerized with the hydrophilic monomer N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine (SPDA). The chromatographic properties of the three hydrophilic sulfoalkylbetaine-type monolithic columns, including column efficiency, permeability, porosity and separation mechanism, were systematically compared using scanning electron microscopy or micro-HPLC. Good selectivity in micro-HPLC separations was achieved on all three monolithic columns. The results indicate that the polarity of sulfoalkylbetaine-type monolithic columns may be related to the polarity of the crosslinker, which further affects column selectivity and efficiency. A particularly high column efficiency (100,000 plates/m) was obtained on the novel poly(SPDA-co-PDA) monolithic column at a linear velocity of 1mm/s using thiourea as test analyte. A higher resolution was also observed for nucleobases, nucleosides and hydrophilic organic acids on this novel poly(SPDA-co-PDA) monolithic column compared to the other two columns.

Research Center/Unit :

Centre Interfacultaire de Recherche du Médicament - CIRM

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Liu, Chusheng

Chen, Weijia

Yuan, Guangxin

Xiao, Yao

Crommen, Jacques ; Université de Liège > Département de pharmacie > Département de pharmacie

Xu, Shihai

Jiang, Zhengjin

Language :

English

Title :

Influence of the crosslinker type on the chromatographic properties of hydrophilic sulfoalkylbetaine-type monolithic columns.

Publication date :

2014

Journal title :

Journal of Chromatography. A

ISSN :

0021-9673

eISSN :

1873-3778

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

1373

Pages :

73-80

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 24 October 2015

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