aTRAQ; amino acids; inborn errors of metabolism; mass spectrometry; validation
Abstract :
[en] Nowadays, physiological amino acids profiling is based primarily on ion exchange chromatography (IEC) coupled to a post-column derivatization with ninhydrin and UV detection at two wavelengths. Unfortunately, this technique suffers various drawbacks such as long analysis time, high sample volume and specific costs related to the maintenance of a dedicated equipment. These reasons have led us to consider a technology switch to a mass spectrometry method. We tested the kit aTRAQ amino acids analysis for physiological samples (AB Sciex), offering a selective quantification of more than 40 amino acids, and have implemented the acquisition of various original markers to the initial method. The accuracy profiles established for each amino acid show that the results are very reliable. The linearity is assured between 1 and 1.000 mumol/L for most analytes. Result comparison with IEC method showed good agreement. Reference ranges are similar to those defined for the IEC method and patients with inborn errors of metabolism were readily identified. The aTRAQ method offers a valid alternative to IEC method with several advantages: reduced sample volume, decreased run time and increased specificity. However, the procedure requires a thorough review of all chromatographic peaks, process that considerably lengthens the overall time of the procedure. Finally, financial and practical considerations of both techniques have to be counterbalanced before initiating any methodological transition.
Disciplines :
Genetics & genetic processes
Author, co-author :
BOEMER, François ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
SCHOOS, Roland ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
DEBERG, Michelle ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
Language :
English
Title :
Quantification of physiological aminoacids using aTRAQ kit: evaluation and implementation of new markers.
Alternative titles :
[en] Quantification des acides amines physiologiques par le kit aTRAQ : evaluation et implementation de nouveaux parametres.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
WHO. International statistical classification of diseases and related health problems. World Health Organization, 2010.
Dietzen DJ, Weindel AL, Carayannopoulos MO, Landt M, Normansell ET, Reimschisel TE, et al. Rapid comprehensive amino acid analysis by liquid chromatography/tandem mass spectrometry : comparison to cation exchange with post-column ninhydrin detection. Rapid Commun Mass Spectrom 2008; 22 : 3481-8.
Duran M. Amino acids. In : Blau N, Duran M, Gibson KM, eds. Laboratory guide to the methods in biochemical genetics. Heidelberg : Springer, 2008, p. 53-89.
Piraud M, Vianey-Saban C, Petritis K, Elfakir C, Steghens JP, Morla A, et al. ESI-MS/MS analysis of underivatised amino acids : A new tool for the diagnosis of inherited disorders of amino acid metabolism. Fragmentation study of 79 molecules of biological interest in positive and negative ionisation mode. Rapid Commun Mass Spectrom 2003; 17 : 1297-311.
Piraud M, Vianey-Saban C, Bourdin C, Acquaviva-Bourdain C, Boyer S, Elfakir C, et al. A new reversed-phase liquid chromatographic/tandem mass spectrometric method for analysis of underivatised amino acids : Evaluation for the diagnosis and the management of inherited disorders of amino acid metabolism. Rapid Commun Mass Spectrom 2005; 19 : 3287- 97.
Waterval WA, Scheijen JL, Ortmans-Ploemen MM, Habets-van der Poel CD, Bierau J. Quantitative UPLC-MS/MS analysis of underivatised amino acids in body fluids is a reliable tool for the diagnosis and follow-up of patients with inborn errors of metabolism. Clin Chim Acta 2009; 407 : 36-42.
Dettmer K, Stevens AP, Fagerer SR, Kaspar H, Oefner PJ. Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS. Methods Mol Biol 2012; 828 : 165-81.
Harder U, Koletzko B, Peissner W. Quantification of 22 plasma amino acids combining derivatization and ion-pair LC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci 2011; 879 : 495-504.
Held PK, White L, Pasquali M. Quantitative urine amino acid analysis using liquid chromatography tandem mass spectrometry and aTRAQ reagents. J Chromatogr B Analyt Technol Biomed Life 2011; 879 : 2695-703.
Gustavo Gonzales A, Angeles Herrador M. Accuracy profiles from uncertainty measurements. Talanta 2006; 70 : 896-901.
Hubert P, Nguyen-Huu JJ, Boulanger B, Chapuzet E, Chiap P, Cohen N, et al. Harmonization of strategies for the validation of quantitative analytical procedures. A SFSTP proposal-Part I. J Pharm Biomed Anal 2004; 36 : 579-86.
Hubert P, Nguyen-Huu JJ, Boulanger B, Chapuzet E, Chiap P, Cohen N, et al. Harmonization of strategies for the validation of quantitative analytical procedures. A SFSTP proposal-Part II. J Pharm Biomed Anal 2007; 45 : 70-81.
Hubert P, Nguyen-Huu JJ, Boulanger B, Chapuzet E, Cohen N, Compagnon PA, et al. Harmonization of strategies for the validation of quantitative analytical procedures. A SFSTP proposal-part III. J Pharm Biomed Anal 2007; 45 : 82-96.
Rozet E1, Morello R, Lecomte F, Martin GB, Chiap P, Crommen J, et al. Performances of a multidimensional on-line SPE-LC-ECD method for the determination of three major catecholamines in native human urine : validation, risk and uncertainty assessments. J Chromatogr B Analyt Technol Biomed Life Sci 2006; 844 : 251-60.
Rozet E, Wascotte V, Lecouturier N, Préat V, Dewé W, Boulanger B, et al. Improvement of the decision efficiency of the accuracy profile by means of a desirability function for analytical methods validation. Application to a diacetyl-monoxime colorimetric assay used for the determination of urea in transdermal iontophoretic extracts. Anal Chim Acta 2007; 591 : 239-47.
Illsinger S, Schmidt KH, Lucke T, Vaske B, Bohnhorst B, Das AM, Plasma AM. urine amino acid pattern in preterm infants on enteral nutrition : Impact of gestational age. Amino Acids 2010; 38 : 959-72.
Rossi R, Danzebrink S, Linnenbürger K, Hillebrand D, GrünebergM, Sablitzky V, et al. Assessment of tubular reabsorption of sodium, glucose, phosphate and amino acids based on spot urine samples. Acta Paediatr 1994; 83 : 1282-6.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.