Three Different Routes of Inoculation for Experimental Infection with Schmallenberg Virus in Sheep

Schmallenberg; Bunyaviridae Infections; Orthobunyavirus; Sheep Diseases; Administration, Intranasal; Animals; Female; Injections, Intradermal; Injections, Subcutaneous; Lymph Nodes; Sheep; Spleen; Vaccination

Abstract :

[en] Schmallenberg virus (SBV) is an emerging Orthobunyavirus affecting European domestic ruminants. In this study, three groups of ewes (n = 3) were inoculated with 1 ml of an SBV infectious serum, via the subcutaneous (SC), intradermal (ID) or intranasal (IN) route. The ewes were monitored for 10 days and no clinical signs were reported. IN inoculation failed to generate any detectable RNAemia. SC and ID inoculation induced typical SBV RNAemia and seroconversion upon day 6 post-inoculation in 3/3 and 2/3 sheep, respectively. In all the animals that showed RNAemia, the viral genome could be detected in spleen and mesenteric lymph nodes. Both the SC and ID routes seem suitable to properly reproduce field conditions, as comparable observations were reported regarding RNAemia, seroconversion and viral genome detection in organs. © 2015 Blackwell Verlag GmbH

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Martinelle, Ludovic ; Université de Liège - ULiège > Station ferme expérimentale (CARE)

Poskin, Antoine ; Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium, Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate Viral Diseases, Brussels, Belgium

Dal Pozzo, Fabiana ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.

Mostin, L.; Veterinary and Agrochemical Research Centre (CODA-CERVA), Experimental Centre, Machelen, Belgium

Van Campe, W.; Veterinary and Agrochemical Research Centre (CODA-CERVA), Experimental Centre, Machelen, Belgium

Cay, A. B.; Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate Viral Diseases, Brussels, Belgium

De Regge, N.; Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate Viral Diseases, Brussels, Belgium

Saegerman, Claude ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.

Language :

English

Title :

Three Different Routes of Inoculation for Experimental Infection with Schmallenberg Virus in Sheep

Publication date :

2017

Journal title :

Transboundary and Emerging Diseases

ISSN :

1865-1674

eISSN :

1865-1682

Publisher :

Wiley, Berlin, Germany

Volume :

Issue :

Pages :

305-308

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 22 September 2015

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