Cell Models Adapted to Real-Time Imaging of the Cytoskeleton Dynamics in Altered Gravity

Willems, Jérôme; Deroanne, Christophe; Colige, Alain; Garbacki, Nancy

doi:10.1007/s12217-014-9392-y

Request a copy

Article (Scientific journals)

Cell Models Adapted to Real-Time Imaging of the Cytoskeleton Dynamics in Altered Gravity

Willems, Jérôme; Deroanne, Christophe; Colige, Alain et al.

2014 • In Microgravity Science and Technology, 26 (4), p. 257-270

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/185219

DOI
10.1007/s12217-014-9392-y

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

Willems J. et al, MGST, 2014.pdf

Publisher postprint (2.62 MB)

Request a copy

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Actin; Bone; Cytoskeleton dynamics; Real-time microscopy; Tubulin

Abstract :

[en] Spatial and temporal regulation of cell phenotype by mechanical forces is a growing field of research in health sciences since these stimuli influence cellular functions, such as proliferation, migration, differentiation and gene expression. In the context of the Fluolive project selected by the European Space Agency and aiming at evaluating the impact of gravity alterations on the cell phenotype, we have developed new bone-derived cell lines adapted for live-cell imaging of the cytoskeleton. Osteoblastic cells derived from human osteosarcomas were used as experimental models. U2-OS and SaoS-2 cells stably expressing TagGFP2- β-actin and mCherry- α-tubulin were established and single-cell clonal cultures were characterized in terms of recombinant proteins production and localization, fluorescence intensity, cell proliferation and migration rates. Living fluorescently-tagged cell lines allow real-time fluorescence microscopy of the cytoskeleton dynamics without bleaching and without alteration of cell morphology. U2-OS and SaoS-2 TagGFP2- β-actin and mCherry- α-tubulin clones will be used to monitor the effect of mechanical forces in models of altered gravity on Earth and possibly on the ISS. © 2014, Springer Science+Business Media Dordrecht.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Willems, Jérôme ; Université de Liège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs

Deroanne, Christophe ; Université de Liège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs

Colige, Alain ; Université de Liège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs

Garbacki, Nancy ; Université de Liège - ULiège

Language :

English

Title :

Cell Models Adapted to Real-Time Imaging of the Cytoskeleton Dynamics in Altered Gravity

Publication date :

2014

Journal title :

Microgravity Science and Technology

ISSN :

0938-0108

eISSN :

1875-0494

Publisher :

Springer, Dordrecht, Netherlands

Volume :

Issue :

Pages :

257-270

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 August 2015

Statistics

Number of views

64 (8 by ULiège)

Number of downloads

2 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Akhmanova, A., Stehbens, S.J., Yap, A.S.: Touch, grasp, deliver and control: Functional cross-talk between microtubules and cell adhesions. Traffic 10(3), 268–274 (2009). doi:10.1111/j.1600-0854.2008.00869.x
Ballestrem, C., Wehrle-Haller, B., Imhof, B.A.: Actin dynamics in living mammalian cells. J. Cell. Sci. 111(Pt 12), 1649–1658 (1998)
Becker, J., Schuppan, D., Benzian, H., Bals, T., Hahn, E.G., Cantaluppi, C., Reichart, P.: Immunohistochemical distribution of collagens types IV, V, and VI and of pro-collagens types I and III in human alveolar bone and dentine. J. Histochem. Cytochem. 34(11), 1417–1429 (1986)
Chhabra, E.S., Higgs, H.N.: The many faces of actin: Matching assembly factors with cellular structures. Nat. Cell. Biol. 9(10), 1110–1121 (2007). doi:10.1038/ncb1007-1110
Chichester, C.O., Fernandez, M., Minguell, J.J.: Extracellular matrix gene expression by human bone marrow stroma and by marrow fibroblasts. Cell Adhes. Commun. 1(2), 93–99 (1993)
Chiquet, M., Gelman, L., Lutz, R., Maier, S.: From mechanotransduction to extracellular matrix gene expression in fibroblasts. Biochim. Biophys. Acta 1793(5), 911–920 (2009). doi:10.1016/j.bbamcr.2009.01.012
Cooper, J.A.: Effects of cytochalasin and phalloidin on actin. J. Cell Biol. 105(4), 1473–1478 (1987)
Czekanska, E.M., Stoddart, M.J., Richards, R.G., Hayes, J.S.: In search of an osteoblast cell model for in vitro research. Eur. Cell Mater. 24, 1–17 (2012)
Discher, D.E., Janmey, P., Wang, Y.L.: Tissue cells feel and respond to the stiffness of their substrate. Science 310(5751), 1139–1143 (2005). doi:10.1126/science.1116995
Emi, N., Friedmann, T., Yee, J.K.: Pseudotype formation of murine leukemia virus with the G protein of vesicular stomatitis virus. J. Virol. 65(3), 1202–1207 (1991)
Etienne-Manneville, S.: Actin and microtubules in cell motility: Which one is in control? Traffic 5(7), 470–477 (2004). doi:10.1111/j.1600-0854.2004.00196.x
Etienne-Manneville, S.: Microtubules in cell migration. Annu. Rev. Cell Dev. Biol. 29, 471–499 (2013). doi:10.1146/annurev-cellbio-101011-155711
Even-Ram, S., Doyle, A.D., Conti, M.A., Matsumoto, K., Adelstein, R.S., Yamada, K.M.: Myosin IIA regulates cell motility and actomyosin-microtubule crosstalk. Nat. Cell Biol. 9(3), 299–309 (2007). doi:10.1038/ncb1540
Fletcher, D.A., Mullins, R.D.: Cell mechanics and the cytoskeleton. Nature 463 (7280), 485–492 (2010)
Frantz, C., Stewart, K.M., Weaver, V.M.: The extracellular matrix at a glance. J. Cell Sci. 123(Pt 24), 4195–4200 (2010). doi:10.1242/jcs.023820
Geeraert, C., Ratier, A., Pfisterer, S.G., Perdiz, D., Cantaloube, I., Rouault, A., Pattingre, S., Proikas-Cezanne, T., Codogno, P., Pous, C.: Starvation-induced hyperacetylation of tubulin is required for the stimulation of autophagy by nutrient deprivation. J. Biol. Chem. 285(31), 24184–24194 (2010). doi:10.1074/jbc.M109.091553
Guignandon, A., Akhouayri, O., Laroche, N., Lafage-Proust, M.H., Alexandre, C., Vico, L.: Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions. Adv. Space Res. 32(8), 1561–1567 (2003). doi:10.1016/s0273-1177(03)90396-6
Guignandon, A., Faure, C., Neutelings, T., Rattner, A., Mineur, P., Linossier, M.T., Laroche, N., Lambert, C., Deroanne, C., Nusgens, B., Demets, R., Colige, A., Vico, L.: Rac1 GTPase silencing counteracts microgravity-induced effects on osteoblastic cells. FASEB journal: Official publication of the Federation of American Societies for Experimental Biology doi:10.1096/fj.14-249714 (2014)
Halper, J., Kjaer, M.: Basic components of connective tissues and extracellular matrix: Elastin, fibrillin, fibulins, fibrinogen, fibronectin, laminin, tenascins and thrombospondins. Adv. Exp. Med. Biol. 802, 31–47 (2014). doi:10.1007/978-94-007-7893-1_3
Horgan, C.P., McCaffrey, M.W.: Rab GTPases and microtubule motors. Biochem. Soc. Trans. 39(5), 1202–1206 (2011). doi:10.1042/BST0391202
Ingber, D.E.: Mechanobiology and diseases of mechanotransduction. Ann. Med. 35(8), 564–577 (2003a)
Ingber, D.E.: Tensegrity I. cell structure and hierarchical systems biology. J. Cell Sci. 116(Pt 7), 1157–1173 (2003b)
Jaalouk, D.E., Lammerding, J.: Mechanotransduction gone awry. Nat. Rev. Mol. Cell Biol. 10(1), 63–73 (2009). doi:10.1038/nrm2597
Janmey, P.A., Miller, R.T.: Mechanisms of mechanical signaling in development and disease. J. Cell Sci. 124(Pt 1), 9–18 (2011). doi:10.1242/jcs.071001
Johnson, I.D.: Practical considerations in the selection and application of fluorescent probes. In: Handbook of Biological Confocal Microscopy, pp. 353-367. Springer (2006)
Johnson, R.B.: The bearable lightness of being: Bones, muscles, and spaceflight. Anat. Rec. 253(1), 24–27 (1998)
Kartsogiannis, V., Ng, K.W.: Cell lines and primary cell cultures in the study of bone cell biology. Mol. Cell Endocrinol. 228(1-2), 79–102 (2004). doi:10.1016/j.mce.2003.06.002
Kline-Smith, S.L., Walczak, C.E.: Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics. Mol. Cell 15(3), 317–327 (2004). doi:10.1016/j.molcel.2004.07.012
Lang, T., LeBlanc, A., Evans, H., Lu, Y., Genant, H., Yu, A.: Cortical and trabecular bone mineral loss from the spine and hip in long-duration spaceflight. J. Bone Miner. Res. Off. J. Amer. Soc. Bone Miner. Res. 19(6), 1006–1012 (2004). doi:10.1359/jbmr.040307
Lanyon, L.E.: Osteocytes, strain detection, bone modeling and remodeling. Calcif Tissue Int 53 Suppl 1, S102–106; discussion S106–107 (1993)
Mammoto, A., Mammoto, T., Ingber, D.E.: Mechanosensitive mechanisms in transcriptional regulation. J. Cell Sci. 125(Pt 13), 3061–3073 (2012). doi:10.1242/jcs.093005
Martin, R.B.: The importance of mechanical loading in bone biology and medicine. J. Musculoskelet Neuronal Interact 7(1), 48–53 (2007)
Mitchison, T., Kirschner, M.: Dynamic instability of microtubule growth. Nature 312(5991), 237–242 (1984)
Mounier, N., Perriard, J.C., Gabbiani, G., Chaponnier, C.: Transfected muscle and non-muscle actins are differentially sorted by cultured smooth muscle and non-muscle cells. J. Cell Sci. 110(Pt 7), 839–846 (1997)
Nabavi, N., Khandani, A., Camirand, A., Harrison, R.E.: Effects of microgravity on osteoclast bone resorption and osteoblast cytoskeletal organization and adhesion. Bone 49(5), 965–974 (2011). doi:10.1016/j.bone.2011.07.036
Pautke, C., Schieker, M., Tischer, T., Kolk, A., Neth, P., Mutschler, W., Milz, S.: Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 24(6), 3743–3748 (2004)
Pollard, T.D., Borisy, G.G.: Cellular motility driven by assembly and disassembly of actin filaments. Cell 112(4), 453–465 (2003)
Ridley, A.J.: Life at the leading edge. Cell 145(7), 1012–1022 (2011). doi:10.1016/j.cell.2011.06.010
Roca-Cusachs, P., Iskratsch, T., Sheetz, M.P.: Finding the weakest link: Exploring integrin-mediated mechanical molecular pathways. J. Cell Sci. 125(Pt 13), 3025–3038 (2012). doi:10.1242/jcs.095794
Sadoshima, J., Jahn, L., Takahashi, T., Kulik, T.J., Izumo, S.: Molecular characterization of the stretch-induced adaptation of cultured cardiac cells. An in vitro model of load-induced cardiac hypertrophy. J. Biol. Chem. 267(15), 10551–10560 (1992)
Salbreux, G., Charras, G., Paluch, E.: Actin cortex mechanics and cellular morphogenesis. Trends Cell Biol. 22(10), 536–545 (2012) doi:10.1016/j.tcb.2012.07.001
Schambach, A., Zychlinski, D., Ehrnstroem, B., Baum, C.: Biosafety features of lentiviral vectors. Hum. Gene Ther. 24(2), 132–142 (2013). doi:10.1089/hum.2012.229
Schuh, M.: An actin-dependent mechanism for long-range vesicle transport. Nat. Cell Biol. 13(12), 1431–1436 (2011). doi:10.1038/ncb2353
Schwarz, U.S., Gardel, M.L.: United we stand: Integrating the actin cytoskeleton and cell-matrix adhesions in cellular mechanotransduction. J. Cell Sci. 125(Pt 13), 3051–3060 (2012). doi:10.1242/jcs.093716
Shaner, N.C., Campbell, R.E., Steinbach, P.A., Giepmans, B.N., Palmer, A.E., Tsien, R.Y.: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22(12), 1567–1572 (2004). doi:10.1038/nbt1037
Sorger, P.K., Dobles, M., Tournebize, R., Hyman, A.A.: Coupling cell division and cell death to microtubule dynamics. Curr. Opin. Cell Biol. 9(6), 807–814 (1997)
Stehbens, S., Wittmann, T.: Targeting and transport: How microtubules control focal adhesion dynamics. J Cell Biol 198(4), 481–489 (2012). doi:10.1083/jcb.201206050
Stricker, J., Falzone, T., Gardel, M.L.: Mechanics of the F-actin cytoskeleton. J. Biomech. 43(1), 9–14 (2010). doi:10.1016/j.jbiomech.2009.09.003
Subach, O.M., Gundorov, I.S., Yoshimura, M., Subach, F.V., Zhang, J., Gruenwald, D., Souslova, E.A., Chudakov, D.M., Verkhusha, V.V.: Conversion of red fluorescent protein into a bright blue probe. Chem. Biol. 15(10), 1116–1124 (2008). doi:10.1016/j.chembiol.2008.08.006
Tojkander, S., Gateva, G., Lappalainen, P.: Actin stress fibers–assembly, dynamics and biological roles. J. Cell Sci. 125(Pt 8), 1855–1864 (2012). doi:10.1242/jcs.098087
Van Loon, J., Krause, J., Cunha, H., Goncalves, J., Almeida, H., Schiller, P.: The large diameter centrifuge, LDC, for life and physical sciences and technology. In: Proceedings of the ‘Life In Space For Life On Earth Symposium’, pp. 22–27. Angers (2008)
van Loon, J.J.: Some history and use of the random positioning machine, RPM, in gravity related research. Adv. Space Res. 39(7), 1161–1165 (2007)
Vassy, J., Portet, S., Beil, M., Millot, G., Fauvel-Lafeve, F., Gasset, G., Schoevaert, D.: Weightlessness acts on human breast cancer cell line MCF-7. Adv. Space Res. 32(8), 1595–1603 (2003)
Vicente-Manzanares, M., Ma, X., Adelstein, R.S., Horwitz, A.R.: Non-muscle myosin II takes centre stage in cell adhesion and migration. Nat. Rev. Mol. Cell Biol. 10(11), 778–790 (2009). doi:10.1038/nrm2786
Wang, N., Tytell, J.D., Ingber, D.E.: Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus. Nat. Rev. Mol. Cell Biol. 10(1), 75–82 (2009). doi:10.1038/nrm2594
Wanisch, K., Yanez-Munoz, R.J.: Integration-deficient lentiviral vectors: a slow coming of age. Mol. Ther. 17(8), 1316–1332 (2009). doi:10.1038/mt.2009.122
Wittmann, T., Waterman-Storer, C.M.: Cell motility: Can Rho GTPases and microtubules point the way? J. Cell Sci. 114(Pt 21), 3795–3803 (2001)