Reference : Interlaboratory study to evaluate the robustness of capillary electrophoresis-mass sp...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/185033
Interlaboratory study to evaluate the robustness of capillary electrophoresis-mass spectrometry for peptide mapping
English
Wenz, Christian mailto [Agilent Technologies R&D and Marketing GmbH & Co. KG, Waldbronn, Germany > > > >]
Barbas, Coral [CEMBIO San Pablo-CEU University, Madrid, Spain > > > >]
Lopez-Gonzalvez, Angeles [CEMBIO San Pablo-CEU University, Madrid, Spain > > > >]
Garcia, Antonia [CEMBIO San Pablo-CEU University, Madrid, Spain > > > >]
Benevente, Fernando [University of Barcelona, Dept. of Analytical Chemistry, Barcelona, Spain > > > >]
Sanz-Nebot, Victoria [University of Barcelona, Dept. of Analytical Chemistry, Barcelona, Spain > > > >]
Blanc, Tim [Eli Lilly and Company, Bioanalytical Science, Branchburg, NJ, USA > > > >]
Freckleton, Gordon [Eli Lilly and Company, Bioanalytical Science, Branchburg, NJ, USA > > > >]
Britz-McKibbin, Philip [McMaster University, Dept. of Chemistry & Chemical Biology, Hamilton, Canada > > > >]
Shanmuganathan, Meera [McMaster University, Dept. of Chemistry & Chemical Biology, Hamilton, Canada > > > >]
de l'Escaille, François mailto [ANALIS s.a., Suarlée (NAMUR), Belgium > > > >]
Far, Johann mailto [Université de Liège > Chemistry > Laboratory of Mass Spectrometry > Ph.D >]
Haselberg, Rob [VU University Amsterdam, Division of BioAnalytical Chemistry, Amsterdam, the Netherlands / University of Tasmania, School of Chemistry, Hobart, Australia > > > >]
Huang, Sean [Amgen Inc., PKDM, Seattle, WA, USA > > > >]
Huhn, Carolin [University of Tuebingen, Institute for Physical and Theoretical Chemistry, Tuebingen, Germany > > > >]
Pattky, Martin [University of Tuebingen, Institute for Physical and Theoretical Chemistry, Tuebingen, Germany > > > >]
Michels, David [Genentech, Inc., Protein Analytical Chemistry Department, San Francisco, CA, USA > > > >]
Mou, Si [Genentech, Inc., Protein Analytical Chemistry Department, San Francisco, CA, USA > > > >]
Yang, Feng [Genentech, Inc., Protein Analytical Chemistry Department, San Francisco, CA, USA > > > >]
Neusuess, Christian [Aalen University, Chemistry Department, Aalen, Germany > > > >]
Tromsdorf, Nora [Aalen University, Chemistry Department, Aalen, Germany > > > >]
Baidoo, Edward E.K [Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, USA > > > >]
Keasling, Jay D. [Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, USA > > > >]
Suhr Park, Sungae [Amgen Inc., Drug Product Development, P&PD, Thousand Oaks, CA, USA > > > >]
11-Aug-2015
Journal of Separation Science
Wiley-VCH Verlag Gmbh
Yes (verified by ORBi)
International
1615-9306
1615-9314
Weinheim
Germany
[en] Precision ; Repeatability ; Reproducibility ; System suitability test ; Tryptic digest
[en] A collaborative study on the robustness and portability of a capillary electrophoresis-mass spectrometry method for peptide mapping was performed by an international team, consisting of 13 independent laboratories from academia and industry. All participants used the same batch of samples, reagents and coated capillaries to run their assays, whereas they utilized the capillary electrophoresis-mass spectrometry equipment available in their laboratories. The equipment used varied in model, type and instrument manufacturer. Furthermore, different types of sheath-flow capillary electrophoresis–mass spectrometry interfaces were used. Migration time, peak height and peak area of ten representative target peptides of trypsin-digested bovine serum albumin were determined by every laboratory on two consecutive days. The data were critically evaluated to identify outliers and final values for means, repeatability (precision within a laboratory) and reproducibility (precision between laboratories) were established. For relative migration time the repeatability was between 0.05 and 0.18% RSD and the reproducibility between 0.14 and 1.3% RSD. For relative peak area repeatability and reproducibility values obtained were 3–12 and 9–29% RSD, respectively. These results demonstrate that capillary electrophoresis-mass spectrometry is robust enough to allow a method transfer across multiple laboratories and should promote a more widespread use of peptide mapping and other capillary electrophoresis-mass spectrometry applications in biopharmaceutical analysis and related fields.
Agilent Technologies, Analis S.A Namur (BE), Laboratoire de Spectrométrie de Masse (LSM, ULG)
http://hdl.handle.net/2268/185033
10.1002/jssc.201500551
http://onlinelibrary.wiley.com/doi/10.1002/jssc.201500551/full

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