Rat Gap Junction Connexin-30 Inhibits Proliferation of Glioma Cell Lines

Princen, Frederic; Robe, Pierre; Gros, D.; Jarry-Guichard, T.; Gielen, Jacques; Merville, Marie-Paule; Bours, Vincent

doi:10.1093/carcin/22.3.507

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Rat Gap Junction Connexin-30 Inhibits Proliferation of Glioma Cell Lines

Princen, Frederic; Robe, Pierre; Gros, D. et al.

2001 • In Carcinogenesis, 22 (3), p. 507-13

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https://hdl.handle.net/2268/18484

DOI
10.1093/carcin/22.3.507

PubMed
11238193

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Abstract :

[en] Connexins, the structural components of gap junctions, control cell growth and differentiation and are believed to belong to a family of tumour suppressor genes. Studies on connexin localization in brain showed that several of these proteins were expressed in distinct compartments of the brain in a cell-type specific manner, indicating that different gap junctions play specific roles in the physiology of the mammalian brain. In this report, we first cloned rat connexin-30 cDNA from brain and showed that it was expressed in long-term primary culture of rat astrocytes. In order to examine the potential role of connexin-30 in tumour cell proliferation, we transfected the connexin-30 cDNA into two rat glioma cell lines (9L and C6) which have lost its expression. Transfected clones adequately expressed membrane-bound connexin-30 protein. Connexin-30-expressing clones showed slower growth, lower DNA synthesis and reduced proliferation in soft agar as compared with the parental and control cells. We concluded that connexin-30 may also probably be considered as a tumour suppressor in rat gliomas.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

Princen, Frederic

Robe, Pierre ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Génétique générale et humaine

Gros, D.

Jarry-Guichard, T.

Gielen, Jacques

Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Bours, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Génétique générale et humaine

Language :

English

Title :

Rat Gap Junction Connexin-30 Inhibits Proliferation of Glioma Cell Lines

Publication date :

March 2001

Journal title :

Carcinogenesis

ISSN :

0143-3334

eISSN :

1460-2180

Publisher :

Oxford University Press, United Kingdom

Volume :

Issue :

Pages :

507-13

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 11 August 2009

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