Protective systems against activated oxygen species compared in normal and fully habituated nonorganogenic sugarbeet calluses

Hagège, D.; Kevers, Claire; Salabert, P.; Gaspar, T.

doi:10.1007/BF02823063

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Protective systems against activated oxygen species compared in normal and fully habituated nonorganogenic sugarbeet calluses

Hagège, D.; Kevers, Claire; Salabert, P. et al.

1992 • In In Vitro Cellular and Developmental Biology. Plant, 28 (3), p. 143-147

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10.1007/BF02823063

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Keywords :

Beta vulgaris

Abstract :

[en] The levels of the water-soluble reductants ascorbic acid and glutathione and the activities of the enzymatic antioxidants superoxide dismutase, catalase, ascorbate peroxidase, monodehydroascorbate and dehydroascorbate reductases and glutathione reductase were determined in a fully habituated nonorganogenic sugarbeet callus line (considered a neoplasm) compared with a normal hormone-dependent callus of the same plant. Ascorbic acid was not recovered from either of the two calluses, irrespective of the technique used. Glutathione was titrated at a slightly higher level in the normal callus. Catalase activity was almost nonexistent in the habituated callus. The other enzymes (superoxide dismutase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase, and ascorbate peroxidase) were found to have higher activities in the habituated callus. The results are interpreted as a higher protection of the neoplastic habituated cells against oxygen-free radicals and hydroperoxide-dependent oxidations. Such strong scavenging properties of the habituated cell line could explain previous results already reported, namely the stimulation of cell division at the expense of cell differentiation. © 1992 Tissue Culture Association.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Hagège, D.; Laboratoire de Physiologie et Biochimie végétales, Université de Caen, Caen cedex, F-14032, France

Kevers, Claire ; Université de Liège > Département des sciences de la vie > Département des sciences de la vie

Salabert, P.; Hormonologie fondamentale et appliquée, Institut de Botanique B22, Université de Liège, Sart Tilman, Liège, B-4000, Belgium

Gaspar, T.; Hormonologie fondamentale et appliquée, Institut de Botanique B22, Université de Liège, Sart Tilman, Liège, B-4000, Belgium

Language :

English

Title :

Protective systems against activated oxygen species compared in normal and fully habituated nonorganogenic sugarbeet calluses

Publication date :

1992

Journal title :

In Vitro Cellular and Developmental Biology. Plant

ISSN :

1054-5476

eISSN :

1475-2689

Publisher :

Springer-Verlag

Volume :

Issue :

Pages :

143-147

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 15 July 2015

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