[en] The zinc finger transcription factor Osterix/Sp7 is an essential
regulator of osteoblastogenesis. In mammals, osterix
expression is regulated by Runx2, Msx2 and Dlx5 but recent
findings suggest that also retinoic acid plays an important
role for osteoblast differentiation and function. Yet, how
these and other factors act on the osterix promoter is largely
unknown. Expression, knock-down and promoter analyses
have indicated that the function of Osterix in osteoblasts is
conserved in teleosts and mammals. In the present study, we
have used the teleost medaka to identify and characterize a
region containing potential retinoic acid response elements in
the osterix promoter. We analysed whether this region is
important for activity in osteoblasts in vivo, using transgenic
medaka lines with modified osterix promoter regions. Promoter
activity in vivo and in vitro revealed a short nucleotide
sequence in the promoter with crucial positive regulatory
function. Mutations of this element lead to a complete inactivation
of the osterix promoter in osteoblasts and made it
insensitive to retinoic acid treatment. The comparison with
the regulatory regions of osterix in other species suggests that
the function of this element is highly conserved in vertebrates.
Disciplines :
Genetics & genetic processes
Author, co-author :
Renn, Jörg ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Buettner, A; National University of Singapore > Department of Biological Sciences
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