Isolation of Chlamydomonas reinhardtii mutants with altered mitochondrial respiration by chlorophyll fluorescence measurement.

Massoz, Simon; Larosa, Véronique; Horrion, Bastien; Matagne, René-Fernand; Remacle, Claire; Cardol, Pierre

doi:10.1016/j.jbiotec.2015.05.009

Article (Scientific journals)

Isolation of Chlamydomonas reinhardtii mutants with altered mitochondrial respiration by chlorophyll fluorescence measurement.

Massoz, Simon; Larosa, Véronique; Horrion, Bastien et al.

2015 • In Journal of Biotechnology

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/182890

DOI
10.1016/j.jbiotec.2015.05.009

PubMed
26022424

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Keywords :

Fluorescence screening; Insertional mutants; Respiratory mutants

Abstract :

[en] The unicellular green alga Chlamydomonas reinhardtii is a model organism for studying energetic metabolism. Most mitochondrial respiratory-deficient mutants characterized to date have been isolated on the basis of their reduced ability to grow in heterotrophic conditions. Mitochondrial deficiencies are usually partly compensated by adjustment of photosynthetic activity and more particularly by transition to state 2. In this work, we explored the opportunity to select mutants impaired in respiration and/or altered in dark metabolism by measuring maximum photosynthetic efficiency by chlorophyll fluorescence analyses (FV/FM). Out of about 2900 hygromycin-resistant insertional mutants generated from wild type or from a mutant strain deficient in state transitions (stt7 strain), 22 were found to grow slowly in heterotrophic conditions and 8 of them also showed a lower FV/FM value. Several disrupted coding sequences were identified, including genes coding for three different subunits of respiratory-chain complex I (NUO9, NUOA9, NUOP4) or for isocitrate lyase (ICL1). Overall, the comparison of respiratory mutants obtained in wild-type or stt7 genetic backgrounds indicated that the FV/FM value can be used to isolate mutants severely impaired in dark metabolism.

Disciplines :

Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Massoz, Simon

Larosa, Véronique ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues

Horrion, Bastien

Matagne, René-Fernand ; Université de Liège > Relations académiques et scientifiques (Sciences)

Remacle, Claire ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues

Cardol, Pierre ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues

Language :

English

Title :

Isolation of Chlamydomonas reinhardtii mutants with altered mitochondrial respiration by chlorophyll fluorescence measurement.

Publication date :

2015

Journal title :

Journal of Biotechnology

ISSN :

0168-1656

eISSN :

1873-4863

Publisher :

Elsevier, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 16 June 2015

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