[en] Broilers were injected at 10 days of age with either Escherichia coli lipopolysaccharides (LPS) or with Freund's adjuvants (FA) to investigate its triggering effect on the acute phase reaction (APR). First the kinetics of certain APP was studied by sampling blood 4 h, 8 h, 12 h and 24 h post injection with LPS. Ovotransferrin (OVT) and ?-1 acid glycoprotein (AGP) concentration increased with time post injection (PI) with LPS to reach a plateau at 12 and 24 h PI. Caeruloplasmin (CP) did not increase with time PI. Compared to injection with phosphate buffered saline, OVT concentrations were higher when injecting chicks with LPS at all time points PI. At 24 h PI, LPS injection resulted in higher OVT and AGP concentration compared to injection with FA. It is recommended to use LPS instead of FA to trigger the APR. The best time point to sample blood for APP determination is 24 h PI. � 2015 Elsevier Ltd.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Koppenol, A.; Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenber 30 Box 2456, Leuven, Belgium, Animal Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Scheldeweg 68, Melle, Belgium
Everaert, Nadia ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Zootechnie
Buyse, J.; Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenber 30 Box 2456, Leuven, Belgium
Delezie, E.; Animal Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Scheldeweg 68, Melle, Belgium
Language :
English
Title :
Challenge with lipopolysaccharides or Freund's adjuvant? What is the best option to trigger acute phase protein production in broilers?
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