Reference : Biosensor based on optical fibers
Scientific congresses and symposiums : Poster
Life sciences : Biotechnology
Biosensor based on optical fibers
[en] Capteur biologique à fibres optiques
Lismont, Marjorie mailto [Université de Liège > Département de physique > Biophotonique >]
Vandewalle, Nicolas mailto [Université de Liège > Département de physique > Physique statistique >]
Joris, Bernard mailto [Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes >]
Dreesen, Laurent mailto [Université de Liège > Département de physique > Biophotonique >]
4th International Conference on Bio-Sensing Technology
10-13 mai 2015
[en] Biocapteur ; Fibres optiques
[en] Medical diagnosis and biotechnology progresses are strongly dependent on the development of sensing devices, which, ideally, would allow the reliable detection of very low amounts of biological species in various environments. In addition to these requirements, the detection tools would also be easy to use and would rapidly response. Fluorescent based biosensors fulfil most of these characteristics.
In our work, the intersection between two crossed optical fibers is used as the basic unit of an original optical biosensor. As illustrated by figure 1, one optical fiber is used to carry probe molecules and excite fluorescence while the second one is devoted to carry the target species and collect the optical signal arising from the species interacting at the node.
The advantages of our set-up over traditional optical sensors are no surface functionalization, use of low amounts of biological species, limitation of the denaturation risk, ease to use and low detection threshold. The developed biosensor is validated on two systems. The first one is a fluorescent calcium indicator, Oregon green 488 BAPTA-2, whose optical emission signal is affected by Ca2+ ions concentration. The second one is based on Rh-Con A and FITC-Dextran complex for which the FRET phenomenon is affected by glucose concentration. In both cases, the results are in agreement with the ones obtained in cuvettes attesting the efficiency of the sensing device.
We also show a prototype of a multichannel device composed of multiple crossed optical fibers which are used as species and light carriers.
Researchers ; Professionals

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