[en] To investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of
Methanol Extract (ME), Water Extract (WE), Acetone Extract (AE), Chloroform Extract (CE), Methanol-Water Extract
(MWE), Methanol-Acetone Extract (MAE), Methanol-Chloroform Extract (MCE) of Ranunculus arvensis (L.).
Antitumor activity was evaluated with Agrobacterium tumefaciens (At10) induced potato disc assay. Cytotoxicity was
evaluated with brine shrimp lethality assay. Antibacterial activity was evaluated with six bacterial strains including
Escherichia coli, Enterobacter aerogenes, Bordetella bronchiseptica, Klebsiella pneumoniae, Micrococcus luteus and
Streptococcus anginosus and antifungal screening was done against five fungal strains including Aspergillus niger, A.
flavus, A. fumigates, Fusarium solani and Mucor species by using disc diffusion method. Best antitumor activity was
obtained with ME and WE, having highest IC50 values 20.27±1.62 and 93.01±1.33μg/disc. Brine shrimp lethality assay
showed LC50 values of AE, MAE and ME were obtained as 384.66±9.42μg/ml, 724.11±8.01μg/ml and 978.7±8.01μg/ml
respectively. WE of R. arvensis revealed weak antimicrobial result against the tested microorganisms. On the other hand,
the antifungal activity of the plant extracts was found to be insignificant. These findings demonstrate that extracts of R.
arvensis possesses significant antitumor activity. Further extensive study is necessary to assess the therapeutic potential
of the plant
Disciplines :
Microbiology
Author, co-author :
Bhatti, Muhammad Zeeshan
Ali, Amjad ; Université de Liège > Département de pharmacie > Chimie médicale
Saeed, Asma
Saeed, Ahmad
Malik, Salman Akbar
Language :
English
Title :
Antimicrobial, antitumor and brine shrimp lethality assay of Ranunculus arvensis L. extracts
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