[en] The microbial community composition in meromictic Lake Kivu, with one of the largest CH4 reservoirs, was studied using 16S rDNA and ribosomal RNA (rRNA) pyrosequencing during the dry and rainy seasons. Highly abundant taxa were shared in a high percentage between bulk (DNA-based) and active (RNA-based) bacterial communities, whereas a high proportion of rare species was detected only in either an active or bulk community, indicating the existence of a potentially active rare biosphere and the possible underestimation of diversity detected when using only one nucleic acid pool. Most taxa identified as generalists were abundant, and those identified as specialists were more likely to be rare in the bulk community. The overall number of environmental parameters that could explain the variation was higher for abundant taxa in comparison to rare taxa. Clustering analysis based on operational taxonomic units (OTUs at 0.03 cutoff) level revealed significant and systematic microbial community composition shifts with depth. In the oxic zone, Actinobacteria were found highly dominant in the bulk community but not in the metabolically active community. In the oxic–anoxic transition zone, highly abundant potentially active Nitrospira and Methylococcales were observed. The co-occurrence of potentially active sulfur-oxidizing and sulfate-reducing bacteria in the anoxic zone may suggest the presence of an active yet cryptic sulfur cycle.
Disciplines :
Microbiology
Author, co-author :
İnceoğlu, Özgul; Université Libre de Bruxelles - ULB > Ecologie des Systèmes Aquatiques
Llirós, Marc; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Laboratory of Freshwater Ecology
Crowe, Sean A.; University of British Columbia - UBC > Departments of Microbiology and Immunology, and Earth, Ocean, and Atmospheric Sciences
García-Armisen, Tamara; Université Libre de Bruxelles - ULB > Ecologie des Systèmes Aquatiques
Morana, Cédric; Katholieke Universiteit Leuven - KUL > Department of Earth and Environmental Sciences
Darchambeau, François ; Université de Liège > Département d'astrophys., géophysique et océanographie (AGO) > Océanographie chimique
Borges, Alberto ; Université de Liège > Département d'astrophys., géophysique et océanographie (AGO) > Océanographie chimique
Descy, Jean-Pierre ; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Laboratory of Freshwater Ecology
Servais, Pierre; Université Libre de Bruxelles - ULB > Ecologie des Systèmes Aquatiques
Language :
English
Title :
Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu
Publication date :
26 April 2015
Journal title :
Microbial Ecology
ISSN :
0095-3628
eISSN :
1432-184X
Publisher :
Springer Verlag, New York, United States - New York
Volume :
70
Issue :
-
Pages :
596–611
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 240002 - AFRIVAL - African river basins: catchment-scale carbon fluxes and transformations.
Name of the research project :
AFRIVAL
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique CE - Commission Européenne
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