Measurement of circulating 25-hydroxyvitamin D: A historical review

[en] The constantly increasing requests for the measurement of serum 25-hydroxyvitamin D over the last years has led reagent manufacturers to market different automated and semi-automated methods, that being unfortunately not fully harmonized, yield different results. Liquid chromatography coupled to tandem mass spectrometry (LC/MS2) has more recently been introduced. This approach allows the distinction between the two forms of 25-hydroxyvitamin D and to measure other metabolites. This approach also requires harmonization to curtail the differences between the different analytical methods. To meet this requirement, the American National Institutes of Health (NIH), the CDC (Centre for Disease Control and Prevention) in Atlanta, the NIST (National Institute of Standards and Technology) and the vitamin D Reference laboratory of Ghent University have pooled their expertise to develop a standardization program. This article reviews the main elements and the difficulties of the automated and semi-automated methods for 25-hydroxyvitamin D, from sample preparation to the analytical phase, as well as those related to mass spectrometry. It also addresses the issues related to the clinical decision thresholds and the possibility of measurements in different biological liquids.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

Delvin, Edgar

LE GOFF, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > Chimie clinique

CAVALIER, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > Chimie clinique

Souberbielle, Jean-Claude

Language :

English

Title :

Measurement of circulating 25-hydroxyvitamin D: A historical review

Publication date :

August 2015

Journal title :

Practical Laboratory Medicine

ISSN :

2352-5517

Publisher :

Elsevier, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 11 May 2015

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