In-vitro fertilization; human sperm counting; Makler chamber
Abstract :
[en] Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very di erent from that observed in the bulk. Swimming along walls is bene cial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells 60 um long, strongly confi ned to 25u m shallow chambers. We investigate the nature of the cell trajectories between the con fining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confi rmed by depth resolved confocal microscopy studies. We show that the introduction of arti cial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an e ffect which we hypothesize may be valuable for micro
fluidic applications in biomedicine.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Guidobaldi, H.A.; Universidad Nacional de Cordoba, Argentina
Jeyaram, Y.; Katholieke Universiteit Leuven - KUL
Condat, C.A.; Universidad Nacional de Cordoba, Argentina
Oviedo, M.; Universidad Nacional de Cordoba, Argentina
Berdakin, I.; Universidad Nacional de Cordoba, Argentina
Moshchalkov, V.V.; Katholieke Universiteit Leuven - KUL
Giojalas, L.C.; Universidad Nacional de Cordoba, Argentina
Silhanek, Alejandro ; Université de Liège > Département de physique > Physique expérimentale des matériaux nanostructurés
Marconi, V.I.; Universidad Nacional de Cordoba, Argentina
Language :
English
Title :
Disrupting the wall accumulation of human sperm cells by artificial corrugation
R. R. Henkel and W. B. Shill, Reprod. Biol. Endocrinol. 1-22, 1081 (2003).
R. R. Henkel, Asian J. Androl. 14, 260 (2012).
L. V. Gatica, H. A. Guidobaldi, M. M. Montesinos, M. E. Teves, A. L. Moreno, D. R. Ũnates, R. I. Molina, L. C. Giojalas, Mol. Human Reprod. 19(9), 559 (2013).
J. B. Y. Koh and Marcos, "The study of spermatozoa and sorting in relation to human reproduction," Microfluid. Nanofluid. (published online 2014).
R. Nosrati, M. Vollmer, L. Eamer, M. C. S. Gabriel, K. Zeidan, A. Zinib, D. Sinton, Lab Chip 14, 1142 (2014).
WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. (WHO Press, Geneva, 2010).
B. S. Cho, T. Schuster, X. Zhu, D. Chang, G. D. Smith, S. Takayama, Anal. Chem. 75, 1671 (2003).
T. G. Schuster, B. Cho, L. M. Keller, S. Takayama, G. D. Smith, RBM Online 7, 75 (2003).
Y. Chung, X. Zhu, W. Gu, G. D. Smith, S. Takayama, in Methods in Molecular Biology: Microfluidic Techniques: Reviews and Protocols, edited by S. D. Minteer (Humana Press Inc., 2006), Vol. 321, p. 227.
D. B. Seo, Y. Agca, Z. C. Feng, J. K. Critser, Microfluid. Nanofluid. 3, 561 (2007).
M. d. C. Lopez-Garcia, R. L. Monson, K. Haubert, M. B. Wheeler, D. J. Beebe, Biomed. Microdev. 10, 709 (2008).
Y. A. Chen et al., Microfluid. Nanofluid. 10, 59 (2011).
C. Y. Chen et al., Analyst 138, 4967 (2013).
G. D. Caprio, A. E. Mallahi, P. Ferraro, R. Dale, G. Coppola, B. Dale, G. Coppola, F. Dubois, Biomed. Opt. Express 5, 690-700 (2014).
L. Rothschild, Nature 198, 1221 (1963).
H. Winet, G. S. Bernstein, J. Head, J. Reprod. Fertil. 70, 511 (1984).
P. Denissenko, V. Kantsler, D. J. Smith, J. Kirkman-Brown, Proc. Natl. Acad. Sci. USA 109, 8007 (2012).
Y. Fily, A. Baskarana, M. F. Hagana, Soft Matter 10, 5609 (2014).
A. Makler, Fertil. Steril. 33(3), 337 (1980).
J. M. S. Frimat, M. Bronkhorst, B. de Wagenaar, J. G. Bomer, F. van der Heijden, A. van den Berg, L. I. Segerink, Lab Chip 14, 2635 (2014).
F. J. H. Hol and C. Dekker, Science 346, 12518211 (2014).
L. I. Segerink, A. J. Sprenkels, P. M. ter Braak, I. Vermes, A. van den Berg, Lab Chip 10, 1018 (2010).
A. P. Berke, L. Turner, H. C. Berg, E. Lauga, Phys. Rev. Lett. 101, 038102 (2008).
G. Li and J. Tang, Phys. Rev. Lett. 103, 0781011 (2009).
E. Lauga and T. R. Powers, Biophys. J. 90, 400 (2009).
J. Elgeti and G. Gompper, Eur. Phys. Lett. 85, 38002 (2009).
J. Elgeti, U. B. Kaupp, G. Gompper, Biophys. J. 99, 1018 (2010).
J. Elgeti and G. Gompper, Eur. Phys. Lett. 101, 48003 (2013).
R. D. Leonardo, D. Dell'Arciprete, L. Angelani, V. Iebba, Phys. Rev. Lett. 106, 038101 (2011).
E. A. Gaffney, H. Gadêlha, D. J. Smith, J. R. Blake, J. C. Kirkman-Brown, Annu. Rev. Fluid Mech. 43, 501 (2011).
J. S. Guasto, R. Rusconi, R. Stocker, Annu. Rev. Fluid Mech. 44, 373 (2012).
J. Dunstan, G. Miño, E. Clement, R. Soto, Phys. Fluids 24, 011901 (2012).
A. Costanzo, J. Elgeti, T. Auth, G. Gompper, M. Ripoll, Eur. Phys. Lett. 107, 36003 (2014).
B. Misselwitz, N. Barrett, S. Kreibich, P. Vonaesch, D. Andritschke, S. Rout, M. S. K. Weidner, P. Songhet, P. Horvath, M. Chabria, V. Vogel, D. M. Spori, P. Jenny, W.-D. Hardt, PLos One 8, e10028101 (2012).
A. Guidobaldi, Y. Jeyaram, I. Berdakin, V. V. Moshchalkov, C. A. Condat, V. I. Marconi, L. Giojalas, A. V. Silhanek, Phys. Rev. E. 89, 032720 (2014).
S. E. Hulme, W. R. DiLuzio, S. S. Shevkoplyas, L. Turner, M. Mayer, H. C. Berg, G. M. Whitesides, Lab Chip 8, 1888-1895 (2008).
I. Berdakin, Y. Jeyaram, V. V. Moshchalkov, L. Venken, S. Dierckx, S. J. Vanderleyden, A. V. Silhanek, C. A. Condat, V. I. Marconi, Phys. Rev. E 87, 052702 (2013).
I. Berdakin, A. V. Silhanek, H. N. M. Cortez, V. I. Marconi, C. A. Condat, Cent. Eur. J. Phys. 11(12), 1653 (2013).
A. Kaiser, H. H. Wensink, H. Löwen, Phys. Rev. Lett. 108, 268307 (2012).
L. Restrepo-Pérez, L. Soler, C. S. Martínez-Cisneros, S. Sánchez, O. G. Schmidt, Lab Chip 14, 1515 (2014).
W. DiLuzio, L. Turner, M. Mayer, P. Garstecki, H. B. D. B. Weibel, G. Whitesides, Nature 435, 1271 (2005).
M. Molaei, M. Barry, R. Stocker, J. Sheng, Phys. Rev. Lett. 113, 068103 (2014).
N. T. Tea, M. Jondet, R. Scholler, "A migration-gravity sedimentation method for collecting motile spermatozoa from human semen," in In Vitro Fertilization, Embryo Transfer and Early Pregnancy (MTP Press Ltd., Lancaster, 1984), pp. 117-120.
J. Cosson, P. Huitorel, C. Gagnon, Cell Motil. Cytoskeleton 54, 56 (2003).
See supplementary material at http://dx.doi.org/10.1063/1.4918979 for examples of cell trajectories and movies associated to Fig. 2.
G. Corkidi, B. Taboada, C. D. Wood, A. Guerrero, A. Darszon, Biochem. Biophys. Res. Commun. 373, 125 (2008).
A. Guerrero, T. Nishigaki, J. Carneiro, Y. Tatsu, C. D. Wood, A. Darszon, Dev. Biol. 344, 52 (2010).
D. M. Woolley, Reproduction 126, 259 (2003).
L. Alvarez, B. M. Friedrich, G. Gompper, U. B. Kaupp, Trends Cell Biol. 24, 198 (2014).
T. W. Su, L. Xue, A. Ozcan, Proc. Natl. Acad. Sci. USA 109, 160181 (2012).
V. Kantsler, J. Dunkel, M. Blayney, R. E. Goldstein, eLife 3, e024031 (2014).
Biomaterials and Medical Device - Associated Infections, 4th ed., edited by L. Barnes and I. R. Cooper (Woodhead Publishing, Elsevier, Cambridge, U.K., 2014).
M. Walls, E. Zuvela, C. Ayres, D. Sherrin, A. Chhotani, L. Butler, K. Peirce, J. Krapez, R. Parker, C. Mooy, P. Mohan, S. Catt, M. Wiltshire, H. W. Bakos, M. Whyte, P. Matson, Asian Pac. J. Reprod. 1(1), 67 (2012).
C. Gasparini, L. W. Simmons, M. Beveridge, J. P. Evans, PLoS ONE 5, e12146 (2010).
G. Miño, Ph.D. thesis, ESPCI ParisTech, 2012.
A. Kudrolli, G. Lumay, D. Volfson, L. S. Tsimring, Phys. Rev. Lett. 100, 058001 (2008).
J. Deseigne, S. Léonard, O. Dauchot, H. Chaté, Soft Matter 8, 5629 (2012).
V. Kantsler, J. Dunkel, M. Polin, R. E. Goldstein, Proc. Natl. Acad. Sci. USA 110, 1187 (2013).
M. Friedrich, I. H. Riedel-Kruse, J. Howard, F. Jülicher, J. Exp. Biol. 213, 1226 (2010).
J. Elgeti, R. G. Winkler, G. Gompper, "Physics of microswimmers: Single particle motion and collective behavior," Prog. Phys. Rep. (submitted); e-print arXiv:1412.2692v1.
H. Shum and E. Gaffney, Phys. Rev. E. 91, 033012 (2015).
