[en] In contrast to most gammaherpesviruses, Bovine herpesvirus 4 (BoHV-4) has a broad range of host species both in vitro and in vivo. Several in vitro studies demonstrated that some human cell lines are sensitive or even permissive to BoHV-4. These observations led to the hypothesis that cross-species transmission of BoHV-4 could lead to human infections. In the present study, we investigate the sensitivity of BoHV-4 to neutralization by naïve human sera in order to determine if humans exhibit innate anti-viral activities against this virus. Our results demonstrate that human sera from naïve individuals, in contrast to the sera of naïve subjects from various animal species, neutralize BoHV-4 efficiently. A series of complementary experiments were performed to unravel the mechanism(s) of this neutralization. The data obtained in this study demonstrates that human serum neutralizes BoHV-4 in a complement dependent manner activated by natural antibodies raised against the Galalpha1-3Galbeta1-4GlcNAc-R epitope expressed by bovine cells
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Machiels, Bénédicte ; Université de Liège - ULiège > Immunologie et vaccinologie
Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie
Brito, Sieberth Do Nascimento
Drion, Pierre ; Université de Liège - ULiège > Services généraux (Faculté de médecine vétérinaire) > Méth. expér. des anim. de labo et éthique en expér. animale
Delforge, Cédric ; Université de Liège - ULiège > Immunologie et vaccinologie
Nizet, Yannick; Université Catholique de Louvain - UCL
Gianello, Pierre; Université Catholique de Louvain - UCL
Bona, Christophe
Costes, Bérénice ; Université de Liège - ULiège > Immunologie et vaccinologie
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