Reference : Removal of C-terminal Src kinase from the immune synapse by a new binding protein
Scientific congresses and symposiums : Paper published in a journal
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/179948
Removal of C-terminal Src kinase from the immune synapse by a new binding protein
English
Mustelin, Tomas []
Rahmouni, Souad mailto [Université de Liège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Alonso, Andres []
Williams, Scott []
Van Stipdonk, Marianne []
Soncini, Chiara []
Schoenberger, Stephan []
2004
Cancer Research
American Association for Cancer Research, Inc. (AACR)
45
Yes (verified by ORBi)
No
International
0008-5472
1538-7445
Baltimore
MD
AACR 95th Annual Meeting 2004
Mar 27–31, 2004
AACR
Orlando
USA
[en] The Csk tyrosine kinase negatively regulates the Src family kinases Lck and Fyn in T cells. Engagement of the T cell antigen receptor results in a removal of Csk from the lipid raft-associated transmembrane protein PAG/Cbp. Instead, Csk becomes associated with a ∼72-kDa tyrosine phosphorylated protein, which we here identify as G3BP, a phosphoprotein reported to bind the SH3 domain of Ras GTPase activating protein. G3BP reduced the ability of Csk to phosphorylate Lck at Y505 by decreasing the amount of Csk in lipid rafts. As a consequence, G3BP augmented T cell activation as measured by IL-2 gene activation. Conversely, elimination of endogenous G3BP by RNA interference reduced TCR signaling. In antigen-specific T cells, endogenous G3BP moved into a intracellular location adjacent to the immune synapse, but well inside the cell, upon antigen recognition. Csk co-localization with G3BP occurred in this ‘parasynaptic’ location. We conclude that G3BP is a new player in TCR signaling that acts to reduce the amount of Csk in the immune synapse.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/179948

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