Article (Scientific journals)
Fluorescent tagging of VP22 in N-terminus reveals that VP22 favors Marek’s disease virus (MDV) virulence in chickens and allows morphogenesis study in MD tumor cells
Remy, Sylvie; Blondeau, Caroline; Le Vern, Yves et al.
2013In Veterinary Research
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Keywords :
Marek's disease virus; VP22; virulence; morphogenesis
Abstract :
[en] Marek’s disease virus (MDV) is an alpha-herpesvirus causing Marek’s disease in chickens, mostly associated with T-cell lymphoma. VP22 is a tegument protein abundantly expressed in cells during the lytic cycle, which is essential for MDV spread in culture. Our aim was to generate a pathogenic MDV expressing a green fluorescent protein (EGFP) fused to the N-terminus of VP22 to better decipher the role of VP22 in vivo and monitor MDV morphogenesis in tumors cells. In culture, rRB-1B EGFP22 led to 1.6-fold smaller plaques than the parental virus. In chickens, the rRB-1B EGFP22 virus was impaired in its ability to induce lymphoma and to spread in contact birds. The MDV genome copy number in blood and feathers during the time course of infection indicated that rRB-1B EGFP22 reached its two major target cells, but had a growth defect in these two tissues. Therefore, the integrity of VP22 is critical for an efficient replication in vivo, for tumor formation and horizontal transmission. An examination of EGFP fluorescence in rRB-1B EGFP22-induced tumors showed that about 0.1% of the cells were in lytic phase. EGFP-positive tumor cells were selected by cytometry and analyzed for MDV morphogenesis by transmission electron microscopy. Only few particles were present per cell, and all types of virions (except mature enveloped virions) were detected unequivocally inside tumor lymphoid cells. These results indicate that MDV morphogenesis in tumor cells is more similar to the morphorgenesis in fibroblastic cells in culture, albeit poorly efficient, than in feather follicle epithelial cells
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Remy, Sylvie;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > UMR1282, Infectious Diseases and Public Health, ISP, BIOVA team, Tours-Nouzilly
Blondeau, Caroline ;  University College London - UCL > Infection and Immunity > Medical Research Council Centre for Medical Molecular Virology
Le Vern, Yves;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > UMR1282, Infectious Diseases and Public Health, ISP, Cytometry facility, Tours-Nouzilly
Lemesle, Monique;  Universite Francois Rabelais, Tours, France > Microscopies (Plateau Technologique Analyse des systèmes Biologiques)
Vautherot, Jean-Francois;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > UMR1282, Infectious Diseases and Public Health, ISP, BIOVA team, Tours-Nouzilly
Denesvre, Caroline;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > UMR1282, Infectious Diseases and Public Health, ISP, BIOVA team, Tours-Nouzilly
Language :
English
Title :
Fluorescent tagging of VP22 in N-terminus reveals that VP22 favors Marek’s disease virus (MDV) virulence in chickens and allows morphogenesis study in MD tumor cells
Publication date :
2013
Journal title :
Veterinary Research
ISSN :
0928-4249
eISSN :
1297-9716
Publisher :
EDP Sciences, Les Ulis, France
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 March 2015

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