[en] Radioresistance and postirradiation repair of potentially lethal damage (PLD repair) are important factors underlying failure to control local disease in cancer. Dipyridamole (DP) is known as a modifier of the action of cytotoxic drugs. We therefore investigated DP as a potential radiosensitizer and inhibitor of PLD repair in X-irradiated Chinese hamster ovary (CHO) cells in vitro. Exposure to the drug alone resulted in a slight reduction of the clonogenic capacity of the cells. Preincubation for 18 h with 10 and 20 microM DP in cells subcultured at low density, led to a significant radiosensitization. In confluent density-inhibited cultures, preincubation alone as well as pre- and postincubation with 20 microM DP resulted in a significant inhibition of PLD repair. Dipyridamole and related compounds may thus be useful tools for modifying and investigating the response of mammalian cells to radiation. [fr] Radiorésistance et la réparation des dommages radio-induits potentiellement létales (réparation PLD) sont des facteurs importants qui sous-tendent l'incapacité à contrôler la maladie locale dans le cancer. Dipyridamole (DP) is known as a modifier of the action of cytotoxic drugs. Dipyridamole (DP) est connu comme un modificateur de l'action des médicaments cytotoxiques. We therefore investigated DP as a potential radiosensitizer and inhibitor of PLD repair in X-irradiated Chinese hamster ovary (CHO) cells in vitro. Nous avons donc enquêté DP comme radiosensibilisant potentiel et de l'inhibiteur de la réparation PLD dans X-irradiated ovaire de hamster chinois (CHO) cells in vitro. Exposure to the drug alone resulted in a slight reduction of the clonogenic capacity of the cells. L'exposition au médicament seul a entraîné une légère réduction de la capacité clonogénique des cellules. Preincubation for 18 h with 10 and 20 microM DP in cells subcultured at low density, led to a significant radiosensitization. Pré-incubation pendant 18 h avec 10 et 20 microM DP dans les cellules sont repiquées à faible densité, conduit à une radiosensibilisation significative. In confluent density-inhibited cultures, preincubation alone as well as pre- and postincubation with 20 microM DP resulted in a significant inhibition of PLD repair. De la densité des cultures confluentes inhibé, préincubation aussi bien seule qu'en pré-et postincubation avec 20 DP microM a entraîné une inhibition significative de la réparation de l'IVC. Dipyridamole and related compounds may thus be useful tools for modifying and investigating the response of mammalian cells to radiation. Dipyridamole et de composés connexes mai donc être des outils utiles pour modifier et à enquêter sur la réponse des cellules de mammifères à des radiations.
Disciplines :
Oncology
Author, co-author :
COUCKE, Philippe ; Centre Hospitalier Universitaire de Liège - CHU > Radiothérapie
Rutz, H-P; Centre Hospitalier Universitaire Vaudois (CHUV) > Radiotherapy
Calmes, J-M; Centre Hospitalier Universitaire Vaudois (CHUV) > Radiotherapy
Mirimanoff, R-O; Centre Hospitalier Universitaire Vaudois (CHUV) > Radiotherapy
Language :
English
Title :
Radiosensitizing and repair-inhibiting properties of dipyridamole.
Alternative titles :
[fr] Radiosensibilisation et de réparation des propriétés d'inhibition de dipyridamole.
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