Sheep; Milk; Plasma; Early pregnancy diagnosis; PAG–RIA system; Number of fetuses
Abstract :
[en] In the present study, four RIA systems (RIA-1 to -4) based on two antisera raised against ovine pregnancyassociated glycoproteins (ovPAGs), combined with an ovine or a bovine PAG tracer were used to measure PAG concentrations in plasma and milk samples of dairy ewes. Blood and milk samples were collected on different days of gestation: 0, 18, 20, 22, 25, 28, 32, 42, and 49. From day 20 onward, the PAG in plasma could be detected in all pregnant ewes using the four RIA systems. By using milk, except for RIA-1, the other systems showed a sensitivity of 100% from day 28 of gestation onward. In plasma, PAG concentrations were higher in multiple than in single pregnancies, while no clear relationship was observed in milk. In conclusion, milk is a good alternative to plasma for early pregnancy diagnosis in sheep from day
28 to day 42.
Research Center/Unit :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
El Amiri, Bouchra; Institut Scientifique de Recherche Agronomique - INRA > Regional Center of Agronomic Research, Settat, Morocco
Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > Physiologie de la reproduction
Alvarez-Oxiley, Andrea; Universidad de la República, Garzón 780, Montevideo, Uruguay > Animal Production and Pasture, Faculty of Agronomy
Hadarbach, D; Institut Scientifique de Recherche Agronomique - INRA > Statistics and Informatics, Rabat, Morocco
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > Département de sciences fonctionnelles (DSF)
Language :
English
Title :
Pregnancy-associated glycoprotein (PAG) concentration in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep
Publication date :
2015
Journal title :
Research in Veterinary Science
ISSN :
0034-5288
eISSN :
1532-2661
Publisher :
British Veterinary Association, London, United Kingdom
Volume :
99
Pages :
30-36
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
BTC - Belgian Technical Cooperation SPW DG03-DGARNE - Service Public de Wallonie. Direction Générale Opérationnelle Agriculture, Ressources naturelles et Environnement F.R.S.-FNRS - Fonds de la Recherche Scientifique ULg FSR - Université de Liège. Fonds spéciaux pour la recherche
Funding text :
Belgian Technical Cooperation (BTC), Work conducted in Belgium was partially supported by grants fromthe SPW-DGARNE (D31-1284), FNRS (Projet CC 1.5155.12), and FSR-ULg (C-02/66).
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