Investigation of Microcystin Congener–Dependent Uptake into Primary Murine Neurons

Animals; Blotting; Immunohistochemistry; Messenger; Messenger: genetics; Mice; Microcystins; Microcystins: genetics; Microcystins: physiology; Neurons; Neurons: physiology; RNA; Western

Abstract :

[en] Contamination of natural waters by toxic cyanobacteria is a growing problem worldwide, resulting in serious water pollution and human health hazards. Microcystins (MCs) represent a group of > 80 cyclic heptapeptides, mediating cytotoxicity via specific protein phosphatase (PP) inhibition at equimolar concentrations (comparable toxicodynamics). Because of the structure and size of MCs, active uptake into cells occurs via organic anion-transporting polypeptides (OATP/Oatp), as confirmed for liver-specific human OATP1B1 and OATP1B3, mouse Oatp1b2 (mOatp1b2), skate Oatp1d1, and the more widely distributed OATP1A2 expressed, for example, at the blood-brain barrier. Tissue-specific and cell-type-specific expression of OATP/Oatp transporters and specific transport of MC congeners (toxicokinetics) therefore appear prerequisite for the reported toxic effects in humans and other species upon MC exposure. Beyond hepatotoxicity induced by the MC-LR congener, the effects of other MC congeners, especially neuronal uptake and toxicity, are unknown.

Disciplines :

Neurology

Author, co-author :

Feurstein, Daniel

Kleinteich, Julia ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes

Heussner, Alexandra H. A. H.

Stemmer, Kerstin

Dietrich, Daniel R.

Language :

English

Title :

Investigation of Microcystin Congener–Dependent Uptake into Primary Murine Neurons

Publication date :

2010

Journal title :

Environmental Health Perspectives

ISSN :

0091-6765

Publisher :

National Institute of Environmental Health Science

Volume :

118

Issue :

Pages :

1370

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2957914&tool=pmcentrez&rendertype=abstract http://ehp03.niehs.nih.gov/article/fetchArticle.action?articleURI=info:doi/10.1289/ehp.0901289 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957914/

Available on ORBi :

since 10 February 2015

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